{"id":248,"date":"2011-05-10T13:31:04","date_gmt":"2011-05-10T04:31:04","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=248"},"modified":"2017-11-11T11:51:05","modified_gmt":"2017-11-11T02:51:05","slug":"%e5%88%86%e8%a3%82%e9%85%b5%e6%af%8d%e3%81%ae%e8%96%ac%e5%89%a4%e8%80%90%e6%80%a7%e9%81%b8%e6%8a%9e%e3%83%9e%e3%83%bc%e3%82%ab%e3%83%bc","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=248","title":{"rendered":"\u5206\u88c2\u9175\u6bcd\u306e\u85ac\u5264\u8010\u6027\u9078\u629e\u30de\u30fc\u30ab\u30fc"},"content":{"rendered":"<p>&nbsp;<\/p>\n<h1 style=\"font-size: 1.3333em; line-height: 1.125em; font-weight: bold; margin: 0.375em 0px 0.375em 0px;\"><span style=\"font-size: 14px; line-height: 20px; color: #111111; font-weight: normal;\"><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16200533\">Three novel antibiotic marker cassettes for gene d&#8230; [Yeast. 2005] &#8211; PubMed result<\/a>.<\/span><\/h1>\n<p>&nbsp;<\/p>\n<p>\u899a\u3048\u66f8\u304d\uff1a\u5206\u88c2\u9175\u6bcd\u3067\u85ac\u5264\u8010\u6027\u3067\u9078\u629e\u3059\u308b\u305f\u3081\u306e\u30de\u30fc\u30ab\u30fc\u306b\u3064\u3044\u3066\u306e\u8ad6\u6587\u3002\u5bcc\u6804\u990a\u57f9\u5730\u3084\u5408\u6210\u57f9\u5730\u3067\u3069\u306e\u6fc3\u5ea6\u3067\u85ac\u5264\u3092\u52b9\u304b\u305b\u305f\u3089\u826f\u3044\u304b\u3001\u305d\u306e\u70ba\u306b\u5229\u7528\u3059\u308b\u30de\u30fc\u30ab\u30fc\u30ab\u30bb\u30c3\u30c8\u907a\u4f1d\u5b50\u306b\u3064\u3044\u3066\u8a18\u8ff0\u3055\u308c\u3066\u3044\u308b\u3002<\/p>\n<p>\u3088\u304f\u4f7f\u308f\u308c\u308b\u3001KanMX &gt; G418\u306b\u52a0\u3048\u3001\u4ee5\u4e0b\u306e\u7d44\u307f\u5408\u308f\u305b\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u3002<\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: arial, helvetica, sans-serif; font-size: 12px; line-height: 18px;\">natMX6 &gt; <\/span><span style=\"color: #000000; font-family: arial, helvetica, sans-serif; font-size: 12px; line-height: 18px;\">nourseothricin (NAT)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: arial, helvetica, sans-serif; font-size: 12px; line-height: 18px;\">hphMX6 &gt; <\/span><span style=\"color: #000000; font-family: arial, helvetica, sans-serif; font-size: 12px; line-height: 18px;\">hygromycin B<\/span><\/li>\n<li><span style=\"color: #000000; font-family: arial, helvetica, sans-serif; font-size: 12px; line-height: 18px;\">bleMX6 &gt; phleomycin<\/span><\/li>\n<\/ul>\n<p>\u3061\u306a\u307f\u306b\u3001MX\u306e\u610f\u5473\u306f\u3001<a href=\"http:\/\/yeast-forum.org\/YGSJwiki\/index.php?ML%A4%CE%C5%EA%B9%C6%A4%AB%A4%E9#z572e5b9\">\u3053\u3061\u3089\u306e\u30b5\u30a4\u30c8<\/a>\u3092\u53c2\u8003\u306b\u3057\u3066\u3082\u3089\u3044\u305f\u3044\uff08\u9a5a\u304d\u306e\u771f\u5b9f\u304c\u96a0\u3055\u308c\u3066\u3044\u308b\uff09\u3002<\/p>\n<p>\u305d\u308c\u306f\u3044\u3044\u3068\u3057\u3066\u3001MX6\u3068\u305d\u308c\u4ee5\u524d\u306eMX\u30b7\u30ea\u30fc\u30ba\u306e\u9055\u3044\u306f\u308f\u305a\u304b\u3067\u3001\u4f55\u3067\u304b\u3048\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u304b\u3063\u305f\u306e\u304b\u306f\u4eca\u3072\u3068\u3064\u3088\u304f\u5206\u304b\u3089\u306a\u3044\u3002\u305f\u3060\u3001\u3053\u306e\u8ad6\u6587\u3067\u300c\u5206\u88c2\u9175\u6bcd\u7528\u306b\u65b0\u3057\u304f\u4f5c\u308a\u307e\u3057\u305f\u300d\u3068\u66f8\u304b\u308c\u3066\u3044\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u3001<a href=\"http:\/\/web.uni-frankfurt.de\/fb15\/mikro\/euroscarf\/data\/pAG25.html\">\u4ee5\u524d\u306b\u300c\u51fa\u82bd\u9175\u6bcd\u7528\u306b\u4f5c\u308a\u307e\u3057\u305f\u300d\u3068\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u3082\u306e<\/a>\u3068\u3001\u308f\u305a\u304b\u6570bp\u3057\u304b\u9055\u308f\u306a\u304f\u3001\u307e\u305f\u305d\u308c\u306f\u5206\u88c2\u9175\u6bcd\u3068\u51fa\u82bd\u9175\u6bcd\u306e\u9055\u3044\u306b\u95a2\u9023\u3057\u305f\u3082\u306e\u3067\u306f\u5168\u304f\u306a\u3044\u3002<\/p>\n<p>\u5f93\u3063\u3066\u3001\u4e21\u8005\u306e\u30ab\u30bb\u30c3\u30c8\u3092\u5206\u88c2\u30fb\u51fa\u82bd\u9175\u6bcd\u3067\u4f7f\u3044\u5206\u3051\u308b\u5fc5\u8981\u306f\u5168\u304f\u306a\u3044\uff08\u306f\u305a\u3060\uff09\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Three no&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3595,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[8,9],"tags":[],"class_list":["post-248","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-8","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/248","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=248"}],"version-history":[{"count":4,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/248\/revisions"}],"predecessor-version":[{"id":3564,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/248\/revisions\/3564"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3595"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=248"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=248"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}