{"id":2928,"date":"2014-05-04T15:10:19","date_gmt":"2014-05-04T06:10:19","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=2928"},"modified":"2017-08-09T21:06:48","modified_gmt":"2017-08-09T12:06:48","slug":"%e9%85%b5%e6%af%8d%e3%81%ae%e5%a2%97%e6%ae%96%e3%83%95%e3%82%a7%e3%83%bc%e3%82%ba%e3%81%a8gfp%e3%81%ae%e8%9b%8d%e5%85%89%e3%81%ae%e5%a4%89%e5%8c%96","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=2928","title":{"rendered":"\u9175\u6bcd\u306e\u5897\u6b96\u30d5\u30a7\u30fc\u30ba\u3068GFP\u306e\u86cd\u5149\u306e\u5909\u5316"},"content":{"rendered":"<p>\u4eca\u56de\u306f\u3001\u300c\u8ad6\u6587\u3068\u3057\u3066\u516c\u958b\u3059\u308b\u307b\u3069\u306e\u30c7\u30fc\u30bf\u30fc\u3067\u306f\u306a\u3044\u304c\u3001\u516c\u958b\u3057\u3066\u304a\u3044\u305f\u3089\u8ab0\u304b\u306e\u5f79\u306b\u7acb\u3064\u304b\u3082\u3057\u308c\u306a\u3044\u5b9f\u9a13\u7d50\u679c\u300d\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u3067\u3059\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\u3001yEGFP\uff08\u9175\u6bcd\u306b\u6700\u9069\u5316\u3057\u305f\u5f37\u5316\u578b\u7dd1\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u3092\u767a\u73fe\u3057\u3066\u3044\u308b\u9175\u6bcd\u7d30\u80de\u306e\u57f9\u990a\u3092\u9577\u6642\u9593\u30e2\u30cb\u30bf\u30fc\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e\u5b9f\u9a13\u306e\u6700\u4e2d\u306b\u3001\u9175\u6bcd\u306e\u5897\u6b96\u901f\u5ea6\u304c\u8457\u3057\u304f\u843d\u3061\u308b\u30d5\u30a7\u30fc\u30ba\uff08\u57f9\u5730\u304b\u3089\u30b0\u30eb\u30b3\u30fc\u30b9\u304c\u67af\u6e07\u3057\u3066\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u3092\u4f7f\u3063\u305f\u5897\u6b96\u306b\u5909\u5316\u3059\u308bDiauxic Shift\uff09\u3067\u3001GFP\u306e\u86cd\u5149\u304c\u8457\u3057\u304f\u843d\u3061\u308b\u3053\u3068\u3092\u3044\u3064\u3082\u89b3\u5bdf\u3057\u3066\u3044\u307e\u3057\u305f\uff08Fig.1\uff09\u3002<\/p>\n<figure id=\"attachment_2929\" aria-describedby=\"caption-attachment-2929\" style=\"width: 500px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/eb2f919a3d8392204459859dfc6a308a.png\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-2929\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/eb2f919a3d8392204459859dfc6a308a-295x300.png\" alt=\"GFP fluorescence during yeast growth\" width=\"500\" height=\"508\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/eb2f919a3d8392204459859dfc6a308a-295x300.png 295w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/eb2f919a3d8392204459859dfc6a308a.png 886w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/a><figcaption id=\"caption-attachment-2929\" class=\"wp-caption-text\">Fig.1 GFP fluorescence during yeast growth<\/figcaption><\/figure>\n<p>\u7dd1\u8272\u306e\u7dda\u304cGFP\u306e\u86cd\u5149\u3001\u9ed2\u3044\u7dda\u304c\u9175\u6bcd\u306e\u5897\u6b96\u3092\u8868\u3057\u3066\u3044\u308b\u3002GFP\u306e\u86cd\u5149\u3068\u9175\u6bcd\u306e\u5897\u6b96\u306f\u3001\u86cd\u5149\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u306730\u5206\u6bce\u306b\u6e2c\u5b9a\u3057\u3066\u3044\u308b\u3002\u8d64\u3044\u77e2\u5370\u306fFig.2\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u3092\u884c\u3063\u305f\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u793a\u3057\u3066\u3044\u308b\u3002<\/p>\n<p>\u300c\u5897\u6b96\u30d5\u30a7\u30fc\u30ba\u304c\u5909\u308f\u3063\u3066GFP\u304c\u6025\u901f\u306b\u5206\u89e3\u3055\u308c\u308b\u300d\u3068\u3044\u3046\u306e\u304c\u4e00\u756a\u3042\u308a\u3048\u305d\u3046\u306a\u306e\u3067\u3001Fig.1\u306e\u77e2\u5370\u306e\u3068\u3053\u308d\u304b\u3089\u4e00\u5b9a\u6570\u306e\u7d30\u80de\u3092\u96c6\u3081\u3001GFP\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u91cf\u3092\u96fb\u6c17\u6cf3\u52d5\u3067\u8abf\u3079\u307e\u3057\u305f\uff08Fig.2\uff09\u3002<\/p>\n<figure id=\"attachment_2930\" aria-describedby=\"caption-attachment-2930\" style=\"width: 450px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d5414220a07e54351ccad11b897ba33f.png\"><img decoding=\"async\" class=\"wp-image-2930\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d5414220a07e54351ccad11b897ba33f.png\" alt=\"Amount of GFP during yeast growth\" width=\"450\" height=\"664\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d5414220a07e54351ccad11b897ba33f.png 554w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d5414220a07e54351ccad11b897ba33f-203x300.png 203w\" sizes=\"(max-width: 450px) 100vw, 450px\" \/><\/a><figcaption id=\"caption-attachment-2930\" class=\"wp-caption-text\">Fig.2 Amount of GFP during yeast growth<\/figcaption><\/figure>\n<p>\u9175\u6bcd\u30bf\u30f3\u30d1\u30af\u8cea\u306eLDS-PAGE\u306b\u3088\u308b\u89e3\u6790\u3002\u77e2\u5370\u306e\u3068\u3053\u308d\u304cGFP\u306b\u3042\u305f\u308b\u3002\u30ec\u30fc\u30f3\u756a\u53f7\u306fFig.1\u306e\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u793a\u3057\u3066\u3044\u308b\u3002\u5de6\u7aef\u306f\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc\u3002<\/p>\n<p>Fig.2\u306e\u77e2\u5370\u306e\u3068\u3053\u308d\u304cGFP\u306b\u5f53\u305f\u308b\u306e\u3067\u3059\u304c\u3001\u7d30\u80de\u306eGFP\u306e\u86cd\u5149\u304c\u4e0b\u304c\u3063\u3066\u3044\u308b\u306b\u3082\u95a2\u308f\u3089\u305a\u3001GFP\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u81ea\u4f53\u306e\u91cf\u306f\u307b\u3068\u3093\u3069\u5909\u308f\u3063\u3066\u3044\u306a\u3044\u3088\u3046\u3067\u3059\u3002\u3064\u307e\u308a\u3001\u9175\u6bcd\u306e\u5897\u6b96\u30d5\u30a7\u30fc\u30ba\u306e\u5909\u5316\u306b\u4f34\u3046GFP\u306e\u86cd\u5149\u306e\u5909\u5316\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u91cf\u305d\u306e\u3082\u306e\u306e\u5909\u5316\u3067\u306f\u306a\u304f\u3001\u4f55\u3089\u304b\u306e\u8cea\u7684\u5909\u5316\u306e\u5f71\u97ff\u306b\u3088\u308b\u3082\u306e\u3060\u3068\u8003\u3048\u3089\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e\u539f\u56e0\u3068\u3057\u3066\u8003\u3048\u3089\u308c\u308b\u30011\u3064\u306e\u53ef\u80fd\u6027\u306f\u7d30\u80de\u5185\u306epH\u306e\u5909\u5316\u3067\u3059\u3002<a title=\"Green fluorescent protein as a noninvasive intracellular pH indicator.\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/9512054\">GFP\u306e\u86cd\u5149\u304cpH\u306b\u611f\u53d7\u6027\u304c\u3042\u308b<\/a>\u3053\u3068\u306f\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u308b\u3053\u3068\u3067\u3059\u3002\u9175\u6bcd\u306eDiauxic shift\u3067\u306f\u3001\u767a\u9175\u304b\u3089\u9178\u7d20\u547c\u5438\u3068\u3044\u3046\u4ee3\u8b1d\u306e\u5927\u304d\u306a\u5909\u5316\u304c\u8d77\u304d\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u305d\u308c\u304c\u7d30\u80de\u5185\u306epH\u306e\u5909\u5316\u3092\u5f15\u304d\u8d77\u3053\u3059\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n<p>\u306a\u304a\u3001\u540c\u3058\u5b9f\u9a13\u3092\u8d64\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308byERFP\u3067\u884c\u3063\u3066\u3082\u86cd\u5149\u306e\u6025\u6fc0\u306a\u73fe\u8c61\u306f\u898b\u3089\u308c\u307e\u305b\u3093\uff08Fig.3\uff09\u3002GFP\u307b\u3069\u306epH\u611f\u53d7\u6027\u304c\u3001RFP\u306b\u306a\u3044\u304b\u3089\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n<figure id=\"attachment_2931\" aria-describedby=\"caption-attachment-2931\" style=\"width: 500px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d696e3183a1adf756719405e64f2c7b4.png\"><img decoding=\"async\" class=\"wp-image-2931\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d696e3183a1adf756719405e64f2c7b4.png\" alt=\"RFP fluorescence during yeast growth\" width=\"500\" height=\"540\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d696e3183a1adf756719405e64f2c7b4.png 840w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2014\/05\/d696e3183a1adf756719405e64f2c7b4-277x300.png 277w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/a><figcaption id=\"caption-attachment-2931\" class=\"wp-caption-text\">RFP fluorescence during yeast growth<\/figcaption><\/figure>\n<p>\u8d64\u8272\u306e\u7dda\u304cRFP\u306e\u86cd\u5149\u3001\u9ed2\u3044\u7dda\u304c\u9175\u6bcd\u306e\u5897\u6b96\u3092\u8868\u3057\u3066\u3044\u308b\u3002RFP\u306e\u86cd\u5149\u3068\u9175\u6bcd\u306e\u5897\u6b96\u306f\u3001\u86cd\u5149\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u306730\u5206\u6bce\u306b\u6e2c\u5b9a\u3057\u3066\u3044\u308b\u3002<\/p>\n<p>\u3044\u305a\u308c\u306b\u305b\u3088\u3001GFP\u3092\u4f7f\u3063\u3066\u907a\u4f1d\u5b50\u767a\u73fe\u306a\u3069\u3092\u30e2\u30cb\u30bf\u30fc\u3057\u3088\u3046\u3068\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u5897\u6b96\u30d5\u30a7\u30fc\u30ba\u306b\u3088\u3063\u3066\u86cd\u5149\u5f37\u5ea6\u304c\u5927\u304d\u304f\u5909\u308f\u308b\u3053\u3068\u3092\u5ff5\u982d\u306b\u5165\u308c\u3066\u304a\u304f\u5fc5\u8981\u304c\u3042\u308b\u3053\u3068\u3092\u3001\u3053\u306e\u7d50\u679c\u306f\u793a\u3057\u3066\u3044\u308b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<div id=\"fastlookup_top\" style=\"display: none;\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4eca\u56de\u306f\u3001\u300c\u8ad6\u6587\u3068\u3057\u3066\u516c\u958b\u3059\u308b\u307b&#8230;<\/p>\n","protected":false},"author":3,"featured_media":2929,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[133,23,9],"tags":[],"class_list":["post-2928","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-133","category-23","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/2928","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2928"}],"version-history":[{"count":7,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/2928\/revisions"}],"predecessor-version":[{"id":3441,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/2928\/revisions\/3441"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/2929"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2928"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2928"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2928"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}