{"id":3067,"date":"2015-04-15T16:39:13","date_gmt":"2015-04-15T07:39:13","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3067"},"modified":"2021-10-21T09:55:11","modified_gmt":"2021-10-21T00:55:11","slug":"%e5%b2%90%e8%b7%af%e3%81%ab%e7%ab%8b%e3%81%a4%e9%85%b5%e6%af%8d%e3%81%ae%e6%a0%84%e9%a4%8a%e8%a6%81%e6%b1%82%e6%80%a7%e3%83%9e%e3%83%bc%e3%82%ab%e3%83%bc","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3067","title":{"rendered":"\u5c90\u8def\u306b\u7acb\u3064\u9175\u6bcd\u306e\u6804\u990a\u8981\u6c42\u6027\u30de\u30fc\u30ab\u30fc"},"content":{"rendered":"<p>\u9175\u6bcd\u306e\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u5b9f\u9a13\u3067\u53e4\u304f\u304b\u3089\u7528\u3044\u3089\u308c\u3066\u304d\u305f\u3082\u306e\u306b\u3001\u6804\u990a\u8981\u6c42\u6027\uff08auxotrophic\uff09\u30de\u30fc\u30ab\u30fc\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u4f8b\u3048\u3070\u3001\u67d3\u8272\u4f53\u4e0a\u306e\u30ed\u30a4\u30b7\u30f3\u306e\u5408\u6210\u9175\u7d20\u306e\u907a\u4f1d\u5b50<em>LEU2<\/em>\u3092\u58ca\u3057\u305f\u682a\u3067\u306f\u3001\u81ea\u8eab\u3067\u30ed\u30a4\u30b7\u30f3\u3092\u5408\u6210\u3067\u304d\u306a\u3044\u305f\u3081\u30ed\u30a4\u30b7\u30f3\u8981\u6c42\u6027\u30fc\u30ed\u30a4\u30b7\u30f3\u306e\u306a\u3044\u57f9\u5730\u3067\u306f\u751f\u3048\u3089\u308c\u306a\u3044\u30fc\u3068\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u682a\u306b\u3001<em>LEU2<\/em>\u3092\u6301\u3063\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5c0e\u5165\u3057\u3066\u3084\u308c\u3070\u30ed\u30a4\u30b7\u30f3\u306e\u306a\u3044\u57f9\u5730\u3067\u3082\u751f\u3048\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u308b\u305f\u3081\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5c0e\u5165\u3055\u308c\u305f\u7d30\u80de\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u51fa\u6765\u307e\u3059\uff08\u3053\u306e\u6642<em>LEU2<\/em>\u306e\u3053\u3068\u3092\u9078\u629e\u30de\u30fc\u30ab\u30fc\u3068\u8a00\u3044\u307e\u3059\uff09\u3002<\/p>\n<p>\u540c\u69d8\u306b\u3001\u30d2\u30b9\u30c1\u30b8\u30f3\u5408\u6210\u9175\u7d20<em>HIS3<\/em>\u3001\u30c8\u30ea\u30d7\u30c8\u30d5\u30a1\u30f3\u5408\u6210\u9175\u7d20<em>TRP1<\/em>\u3001\u30ea\u30b8\u30f3\u5408\u6210\u9175\u7d20<em>LYS2<\/em>\u3001\u30e1\u30c1\u30aa\u30cb\u30f3\u5408\u6210\u9175\u7d20<em>MET17<\/em>\u3001\u30a2\u30c7\u30cb\u30f3\u5408\u6210\u9175\u7d20<em>ADE2<\/em>\u3001\u30a6\u30e9\u30b7\u30eb\u5408\u6210\u9175\u7d20<em>URA3<\/em>\u306a\u3069\u306e\u9078\u629e\u30de\u30fc\u30ab\u30fc\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u6804\u990a\u8981\u6c42\u6027\u3092\u7528\u3044\u305f\u7d44\u63db\u3048\u4f53\u306e\u9078\u629e\u306f\u3001\u5b89\u4fa1\u3067\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u306a\u304f\u3001\u30de\u30fc\u30ab\u30fc\u306e\u6570\u3060\u3051\u3055\u307e\u3056\u307e\u306aDNA\u3092\u5c0e\u5165\u3067\u304d\u308b\u3068\u3044\u3046\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308a\u3001\u9175\u6bcd\u306e\u5206\u5b50\u907a\u4f1d\u5b66\u7684\u7814\u7a76\u306b\u5927\u304d\u306a\u30e1\u30ea\u30c3\u30c8\u3092\u3082\u305f\u3089\u3057\u307e\u3057\u305f\uff08\u3053\u306e\u4ed6\u3001\u30ab\u30a6\u30f3\u30bf\u30fc\u30bb\u30ec\u30af\u30b7\u30e7\u30f3\u3068\u3044\u3046\u6280\u3082\u4f7f\u3048\u307e\u3059\uff09\u3002<\/p>\n<p>\u4e00\u65b9\u3067\u3001\u3053\u306e\u6804\u990a\u8981\u6c42\u6027\u3068\u3044\u3046\u6027\u8cea\u304c\u3001\u9175\u6bcd\u306e\u751f\u7406\u30fc\u7279\u306b\u4ee3\u8b1d\u306b\u69d8\u3005\u306a\u60aa\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3068\u3044\u3046\u3053\u3068\u306f\u60f3\u50cf\u306b\u56fa\u304f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p>\u4f8b\u3048\u3070\u3001<em>LEU2<\/em>\u3092\u7834\u58ca\u3057\u305f\u9175\u6bcd\u3067\u306f\u3001\u30ed\u30a4\u30b7\u30f3\u3092\u5408\u6210\u3059\u308b\u4ed6\u306e\u9175\u7d20\u306f\u3042\u308b\u306e\u306b\u3001<em>LEU2<\/em>\u3060\u3051\u304c\u306a\u3044\u72b6\u614b\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u4e0a\u3067\u3001\u5916\u304b\u3089\u30ed\u30a4\u30b7\u30f3\u3092\u4f9b\u7d66\u3057\u3066\u9175\u6bcd\u3092\u751f\u304b\u3057\u3066\u3044\u308b\u72b6\u614b\u306b\u306a\u308a\u307e\u3059\u3002\u9175\u6bcd\u306e\u4ee3\u8b1d\u306f\u3053\u3046\u3044\u3046\u300c\u60f3\u5b9a\u5916\u300d\u306e\u72b6\u6cc1\u304c\u8d77\u304d\u305f\u6642\u306b\u3069\u3046\u306a\u308b\u306e\u304b\u30fb\u30fb\u30fb\u5b9f\u306f\u3042\u307e\u308a\u308f\u304b\u3063\u3066\u3044\u307e\u305b\u3093\u3002\u3068\u308a\u3042\u3048\u305a\u4fbf\u5229\u3060\u304b\u3089\u6804\u990a\u8981\u6c42\u6027\u30de\u30fc\u30ab\u30fc\u3092\u4f7f\u3063\u3066\u304a\u3053\u3046\u3068\u3044\u3046\u306e\u304c\u3001\u4eca\u307e\u3067\u306e\u7814\u7a76\u306e\u6b74\u53f2\u3067\u3082\u3042\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u3057\u304b\u3057\u3001\u30b7\u30b9\u30c6\u30e0\u751f\u7269\u5b66\u3084\u5408\u6210\u751f\u7269\u5b66\u306e\u6642\u4ee3\u306b\u306a\u3063\u3066\u3001\u9175\u6bcd\u306e\u751f\u7406\u3092\u8a73\u3057\u304f\u8abf\u3079\u308b\u3068\u3001\u6804\u990a\u8981\u6c42\u6027\u304c\u5897\u6b96\u306b\u60aa\u5f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href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11976076?dopt=Abstract\" target=\"_blank\" rel=\"noopener\">\u8ad6\u6587 (1) <\/a>\u304c\u3042\u308a\u307e\u3059\u3002\u5b9f\u9a13\u5ba4\u9175\u6bcd\u306e\u4e2d\u306b\u306f\u5408\u6210\u5b8c\u5168\u57f9\u5730\u3067\u306a\u305c\u304b\u5897\u6b96\u3067\u304d\u306a\u3044\u3082\u306e\u304c\u3042\u308a\u3001\u3053\u308c\u304c\u30ed\u30a4\u30b7\u30f3\u306e\u53d6\u308a\u8fbc\u307f\u304c\u3046\u307e\u304f\u884c\u3063\u3066\u3044\u306a\u3044\u305b\u3044\u3067\u3042\u308b\u3068\u3044\u3046<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/17573937\" target=\"_blank\" rel=\"noopener\">\u8ad6\u6587 (2) <\/a>\u3082\u3042\u308a\u307e\u3059\u3002\u524d\u8005\u306e\u5831\u544a\u306f2002\u5e74\u306e\u3082\u306e\u3067\u3059\u304c\u3001\u3053\u3053\u3067\u3059\u3067\u306b\u300cauxotrophic\u30de\u30fc\u30ab\u30fc\u306e\u4f7f\u7528\u306f\u306a\u308b\u3079\u304f\u907f\u3051\u305f\u65b9\u304c\u3044\u3044\u300d\u3068\u63d0\u8a00\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u6804\u990a\u8981\u6c42\u6027\u306e\u682a\u3092\u5bcc\u6804\u990a\u57f9\u5730\u3067\u57f9\u990a\u3059\u308b\u3068\u304d\u306b\u3082\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3059\u3002<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21120607?dopt=Abstract\" target=\"_blank\" rel=\"noopener\">\u3053\u306e\u8ad6\u6587 (3) <\/a>\u3067\u306f\u3001YPD\u3067\u57f9\u990a\u3059\u308b\u3068\u304d\u306b\u3082\u6804\u990a\u8981\u6c42\u6027\u682a\u306e\u5897\u6b96\u306f\u5236\u9650\u3055\u308c\u3066\u3044\u308b\u3068\u5831\u544a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u305d\u306e\u3088\u3046\u306a\u61f8\u5ff5\u304b\u3089\uff08\uff1f\uff09\u6700\u8fd1\u3067\u306f\u3001\u6804\u990a\u5b8c\u5168\u6027\uff08prototroph\uff09\u306e\u9175\u6bcd\u682a\u3092\u7528\u3044\u305f\u907a\u4f1d\u5b50\u7834\u58ca\u682a\u30bb\u30c3\u30c8\u306e\u69cb\u7bc9\u304c\u884c\u308f\u308c\u3066\u3044\u307e\u3059\uff08\u4f8b\u3048\u3070\u3001<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24721214\" target=\"_blank\" rel=\"noopener\">\u3053\u306e\u8ad6\u6587 (4) <\/a>\u3084<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23222782\" target=\"_blank\" rel=\"noopener\">\u3053\u306e\u8ad6\u6587 (5)<\/a>\uff09\u3002<\/p>\n<p>\u9175\u6bcd\u306e\u5206\u5b50\u907a\u4f1d\u5b66\u3092\u767a\u5c55\u3055\u305b\u308b\u305f\u3081\u306b\u4f7f\u308f\u308c\u3066\u304d\u305f\u6804\u990a\u8981\u6c42\u6027\u30de\u30fc\u30ab\u30fc\u3067\u3059\u304c\u3001\u30b7\u30b9\u30c6\u30e0\u751f\u7269\u5b66\u3067\u306f\u90aa\u9b54\u8005\u306b\u306a\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002<strong>\u6804\u990a\u8981\u6c42\u6027\u304c\u4eca\u3001\u5c90\u8def\u306b\u7acb\u305f\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/strong><\/p>\n<p>\u30fb\u30fb\u30fb\u5c11\u3057\u5927\u3052\u3055\uff1f<\/p>\n<p>\u30fc\u30fc<\/p>\n<p>\u53c2\u8003\u8ad6\u6587<\/p>\n<ol>\n<li>Pronk JT. Auxotrophic yeast strains in fundamental and applied research. Appl Environ Microbiol. 2002 May;68(5):2095-100. doi: 10.1128\/AEM.68.5.2095-2100.2002. PMID: 11976076; PMCID: PMC127579.<\/li>\n<li>Cohen R, Engelberg D. Commonly used Saccharomyces cerevisiae strains (e.g. BY4741, W303) are growth sensitive on synthetic complete medium due to poor leucine uptake. FEMS Microbiol Lett. 2007 Aug;273(2):239-43. doi: 10.1111\/j.1574-6968.2007.00798.x. Epub 2007 Jun 15. PMID: 17573937.<\/li>\n<li>Corbacho I, Teixid\u00f3 F, Vel\u00e1zquez R, Hern\u00e1ndez LM, Olivero I. Standard YPD, even supplemented with extra nutrients, does not always compensate growth defects of Saccharomyces cerevisiae auxotrophic strains. Antonie Van Leeuwenhoek. 2011 Mar;99(3):591-600. doi: 10.1007\/s10482-010-9530-5. Epub 2010 Dec 1. PMID: 21120607.<\/li>\n<li>VanderSluis B, Hess DC, Pesyna C, Krumholz EW, Syed T, Szappanos B, Nislow C, Papp B, Troyanskaya OG, Myers CL, Caudy AA. Broad metabolic sensitivity profiling of a prototrophic yeast deletion collection. Genome Biol. 2014 Apr 10;15(4):R64. doi: 10.1186\/gb-2014-15-4-r64. PMID: 24721214; PMCID: PMC4053978.<\/li>\n<li>M\u00fclleder M, Capuano F, Pir P, Christen S, Sauer U, Oliver SG, Ralser M. A prototrophic deletion mutant collection for yeast metabolomics and systems biology. Nat Biotechnol. 2012 Dec;30(12):1176-8. doi: 10.1038\/nbt.2442. PMID: 23222782; PMCID: PMC3520112.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u9175\u6bcd\u306e\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u5b9f\u9a13\u3067\u53e4\u304f&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3499,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[19,10,25,16,8,9],"tags":[],"class_list":["post-3067","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-19","category-10","category-25","category-16","category-8","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3067","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3067"}],"version-history":[{"count":7,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3067\/revisions"}],"predecessor-version":[{"id":4246,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3067\/revisions\/4246"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3499"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3067"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3067"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3067"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}