{"id":3070,"date":"2015-04-15T17:05:03","date_gmt":"2015-04-15T08:05:03","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3070"},"modified":"2022-12-13T11:16:33","modified_gmt":"2022-12-13T02:16:33","slug":"by%e3%82%b7%e3%83%aa%e3%83%bc%e3%82%ba%e9%85%b5%e6%af%8d%e3%82%92%e5%9f%b9%e9%a4%8a%e3%81%99%e3%82%8b%e3%81%9f%e3%82%81%e3%81%ae%e3%80%8c%e3%82%b4%e3%83%bc%e3%83%ab%e3%83%89%e3%82%b9%e3%82%bf%e3%83%b3","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3070","title":{"rendered":"BY\u30b7\u30ea\u30fc\u30ba\u9175\u6bcd\u682a\u306e\u305f\u3081\u306e\u30b4\u30fc\u30eb\u30c9\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u5b8c\u5168\u57f9\u5730"},"content":{"rendered":"<p>\u5b9f\u9a13\u5ba4\u3067\u306e\u5fae\u751f\u7269\u306e\u57f9\u990a\u306b\u306f\u3001\u305d\u308c\u305e\u308c\u306e\u5fae\u751f\u7269\u306b\u9069\u3057\u305f\u57f9\u5730\u304c\u4f7f\u308f\u308c\u307e\u3059\u3002\u79c1\u305f\u3061\u304c\u4f7f\u3063\u3066\u3044\u308b\u51fa\u82bd\u9175\u6bcd\uff08Saccharomyces cerevisiae\uff09\u306e\u5834\u5408\u3001\u5bcc\u6804\u990a\u57f9\u5730\u3068\u3057\u3066YPD\uff08yeast extract, peptone, dextrose\uff09\u57f9\u5730\u304c\u3001\u5408\u6210\u57f9\u5730\u30fb\u5408\u6210\u5b8c\u5168\u57f9\u5730\u3068\u3057\u3066\u3001SD\uff08synthetic dextrose\uff09\u57f9\u5730\u3084SC\uff08synthetic complete\uff09\u57f9\u5730\u304c\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e\u57f9\u5730\u306e\u300c\u30ec\u30b7\u30d4\u300d\u306f\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u9175\u6bcd\u306e\u5b9f\u9a13\u672c\u306b\u66f8\u3044\u3066\u3042\u308a\u307e\u3059\u3002<\/p>\n<ul>\n<li><a href=\"http:\/\/www.amazon.co.jp\/Methods-Yeast-Genetics-2005-Laboratory\/dp\/0879697288\" target=\"_blank\" rel=\"noopener\">Methods in Yeast Genetics 2005\u00a0<\/a><\/li>\n<li>\n<p id=\"title\" class=\"a-size-large a-spacing-none\"><a href=\"http:\/\/www.amazon.co.jp\/%E3%83%90%E3%82%A4%E3%82%AA%E5%AE%9F%E9%A8%93%E3%82%A4%E3%83%A9%E3%82%B9%E3%83%88%E3%83%AC%E3%82%A4%E3%83%86%E3%83%83%E3%83%89%E3%80%887%E3%80%89%E4%BD%BF%E3%81%8A%E3%81%86%E9%85%B5%E6%AF%8D%E3%81%A7%E3%81%8D%E3%82%8BTwo-Hybrid-%E7%9B%AE%E3%81%A7%E8%A6%8B%E3%82%8B%E5%AE%9F%E9%A8%93%E3%83%8E%E3%83%BC%E3%83%88%E3%82%B7%E3%83%AA%E3%83%BC%E3%82%BA-%E6%B0%B4%E9%87%8E-%E8%B2%B4%E4%B9%8B\/dp\/4879622656\/ref=pd_sxp_f_pt\" target=\"_blank\" rel=\"noopener\"><span id=\"productTitle\" class=\"a-size-large\">\u30d0\u30a4\u30aa\u5b9f\u9a13\u30a4\u30e9\u30b9\u30c8\u30ec\u30a4\u30c6\u30c3\u30c9\u30087\u3009\u4f7f\u304a\u3046\u9175\u6bcd\u3067\u304d\u308bTwo Hybri<\/span><span id=\"productTitle\" class=\"a-size-large\">d<\/span><\/a><\/p>\n<\/li>\n<\/ul>\n<p>\u3053\u3053\u3067\u66f8\u304b\u308c\u3066\u3044\u308b\u300c\u5408\u6210\u57f9\u5730\u300d\u306e\u30ec\u30b7\u30d4\u306f\u3069\u3046\u3084\u3063\u3066\u4f5c\u3089\u308c\u3066\u304d\u305f\u306e\u304b\u30fb\u30fb\u30fb\u79c1\u3082\u53e4\u3044\u6b74\u53f2\u306f\u77e5\u308a\u307e\u305b\u3093\u3002<\/p>\n<p>\u305f\u3060\u3001\u300c\u5408\u6210\u5b8c\u5168\u57f9\u5730\u306b\u306f\u3001\u305f\u3076\u3093\u5897\u6b96\u306b\u5fc5\u8981\u306a\u6804\u990a\u6e90\u306f\u5168\u3066\u305f\u3063\u3077\u308a\u5165\u3063\u3066\u3044\u3066\u3001\u70ad\u7d20\u6e90\u304c\u5207\u308c\u308b\u307e\u3067\u306f\u9175\u6bcd\u306f\u30cf\u30c3\u30d4\u30fc\u306b\u5897\u6b96\u3059\u308b\u306e\u3060\u308d\u3046\u300d\u3068\u697d\u89b3\u7684\u306b\u4fe1\u3058\u3066\u5b9f\u9a13\u306b\u4f7f\u3044\u7d9a\u3051\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u3068\u3053\u308d\u304c\u3001\u300c\u79c1\u305f\u3061\u304c\u65e5\u5e38\u7684\u306b\u4f7f\u3063\u3066\u3044\u308bBY\u30b7\u30ea\u30fc\u30ba\u682a\u3067\u306f\u3001\u4eca\u307e\u3067\u306e\u5b8c\u5168\u57f9\u5730\u306b\u306f\u8db3\u308a\u306a\u3044\u6804\u990a\u7d20\u304c\u3042\u308a\u3001\u9175\u6bcd\u306f\u30cf\u30c3\u30d4\u30fc\u306b\u5897\u6b96\u3057\u3066\u3044\u306a\u3044\u300d\u3001\u3068\u3044\u3046\u8ad6\u6587\u3092\u898b\u3064\u3051\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u3002<\/p>\n<p class=\"title\"><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22780918\">Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth.<\/a><\/p>\n<div class=\"supp\">\n<p class=\"desc\">Hanscho M, Ruckerbauer DE, Chauhan N, Hofbauer HF, Krahulec S, Nidetzky B, Kohlwein SD, Zanghellini J, Natter K.\u00a0<span class=\"jrnl\" title=\"FEMS yeast research\">FEMS Yeast Res<\/span>. 2012 Nov;12(7):796-808. PMID: 22780918<\/p>\n<p class=\"desc\">\u3053\u306e\u8ad6\u6587\u3067\u306f\u3001BY\u30b7\u30ea\u30fc\u30ba\u304cYPD\u3068\u540c\u3058\u304f\u3089\u3044\u30cf\u30c3\u30d4\u30fc\u306b\u5897\u6b96\u3067\u304d\u308b\u3088\u3046\u306b\u5408\u6210\u57f9\u5730\u306e\u6700\u9069\u5316\u3092\u304a\u3053\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u65e2\u5b58\u306e\u30ec\u30b7\u30d4\u3067\u306f\u30a2\u30df\u30ce\u9178\u306e\u3044\u304f\u3064\u304b\u3068\u30a4\u30ce\u30b7\u30c8\u30fc\u30eb\u306e\u91cf\u304c\u8db3\u3089\u306a\u3044\u3088\u3046\u3067\u3059\u3002\u3053\u308c\u3089\u3092\u88dc\u3046\u3053\u3068\u306b\u3088\u308a\u3001\u5897\u6b96\u901f\u5ea6\u3068\u6700\u7d42\u30d0\u30a4\u30aa\u30de\u30b9\u3001\u3055\u3089\u306b\u751f\u5b58\u7387\u304c\u98db\u8e8d\u7684\u306b\u5411\u4e0a\u3059\u308b\u3088\u3046\u3067\u3059\u3002<\/p>\n<p class=\"desc\">\u30ec\u30b7\u30d4\u81ea\u4f53\u306f\u4ee5\u524d\u306e\u3082\u306e\u3068\u6bd4\u3079\u3066\u305d\u308c\u307b\u3069\u8907\u96d1\u3067\u306f\u3042\u308a\u307e\u305b\u3093\uff08\u3080\u3057\u308d\u52a0\u3048\u308b\u30a2\u30df\u30ce\u9178\u306e\u6570\u306f\u6e1b\u3063\u3066\u3044\u307e\u3059\uff09\u3002\u3053\u308c\u304c\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306a\u3069\u304c\u4f5c\u3089\u3048\u3066\u3044\u308bBY4741\u3001BY4742\u306a\u3069\u306eBY\u30b7\u30ea\u30fc\u30ba\u682a\u3092\u4f7f\u3046\u3068\u304d\u306e\u300c\u30b4\u30fc\u30eb\u30c9\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u57f9\u5730\u300d\u306b\u306a\u308b\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n<p class=\"desc\">\u4f59\u8ac7\u3067\u3059\u304c\u3001\u4e0a\u8a18\u306e\u8ad6\u6587\u3067\u306f\u9175\u6bcd\u306e\u4ee3\u8b1d\u3092\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3057\u3066\u3001\u7406\u8ad6\u7684\u306a\u5897\u6b96\u901f\u5ea6\u3084\u6700\u7d42\u30d0\u30a4\u30aa\u30de\u30b9\u3092\u8a08\u7b97\u3057\u3001\u300c\u5b8c\u5168\u57f9\u5730\u3067\u306e\u9175\u6bcd\u306e\u5897\u6b96\u306b\u306f\u8a08\u7b97\u3067\u306f\u8aac\u660e\u3067\u304d\u306a\u3044\u70ad\u7d20\u6e90\u4ee5\u5916\u306e\u5897\u6b96\u306e\u5236\u9650\u56e0\u5b50\u304c\u3042\u308b\u300d\u3053\u3068\u3092\u4e88\u60f3\u3057\u3066\u3044\u307e\u3059\u3002\u30a8\u30ad\u30b5\u30a4\u30c6\u30a3\u30f3\u30b0\u306a\u6642\u4ee3\u306b\u306a\u3063\u305f\u3082\u306e\u3067\u3059\u3002<\/p>\n<p class=\"desc\">\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5b9f\u9a13\u5ba4\u3067\u306e\u5fae\u751f\u7269\u306e\u57f9\u990a\u306b\u306f\u3001\u305d&#8230;<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[10,22,23,8,9],"tags":[],"class_list":["post-3070","post","type-post","status-publish","format-standard","hentry","category-10","category-22","category-23","category-8","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3070","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3070"}],"version-history":[{"count":6,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3070\/revisions"}],"predecessor-version":[{"id":4398,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3070\/revisions\/4398"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3070"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3070"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3070"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}