{"id":3096,"date":"2015-09-16T15:23:42","date_gmt":"2015-09-16T06:23:42","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3096"},"modified":"2015-09-16T15:23:42","modified_gmt":"2015-09-16T06:23:42","slug":"selective-ploidy-ablation-%e3%83%97%e3%83%a9%e3%82%b9%e3%83%9f%e3%83%89%e3%82%92%e3%83%8f%e3%82%a4%e3%82%b9%e3%83%ab%e3%83%bc%e3%83%97%e3%83%83%e3%83%88%e3%81%ab%e7%a7%bb%e3%81%99%e6%96%b9%e6%b3%95","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3096","title":{"rendered":"Selective ploidy ablation: \u30d7\u30e9\u30b9\u30df\u30c9\u3092\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306b\u79fb\u3059\u65b9\u6cd5"},"content":{"rendered":"<p class=\"title\"><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21173034\">Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage.<\/a>\u00a0Reid RJ, Gonz\u00e1lez-Barrera S, Sunjevaric I, Alvaro D, Ciccone S, Wagner M, Rothstein R.\u00a0<span class=\"jrnl\" title=\"Genome research\">Genome Res<\/span>. 2011 Mar;21(3):477-86. doi: 10.1101\/gr.109033.110. Epub 2010 Dec 20.\u00a0PMID:21173034<\/p>\n<div class=\"supp\">\n<p class=\"details\">\u305d\u308c\u307b\u3069\u65b0\u3057\u3044\u8ad6\u6587\u3067\u306f\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u305f\u307e\u305f\u307e\u898b\u3064\u3051\u3066\uff08\u79c1\u305f\u3061\u306b\u3068\u3063\u3066\uff09\u3068\u3066\u3082\u4f7f\u3048\u305d\u3046\u306a\u5b9f\u9a13\u624b\u6cd5\u3067\u3042\u3063\u305f\u306e\u3067\u3001\u899a\u66f8\u3092\u517c\u306d\u3066\u66f8\u3044\u3066\u304a\u304d\u307e\u3059\u3002<\/p>\n<p class=\"details\">\u7c21\u5358\u306b\u8a00\u3046\u3068\u3001\u300c\u51fa\u82bd\u9175\u6bcd\u306e\u682a\u9593\u3067\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306b\u79fb\u52d5\u3055\u305b\u308b\u65b9\u6cd5\u300d\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p 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href=\"http:\/\/www.wako-chem.co.jp\/siyaku\/info\/gene\/article\/DirectTransformationKitWako.htm\">\u3053\u308c\u3092\u52b9\u7387\u3088\u304f\u884c\u3046\u305f\u3081\u306e\u8a66\u85ac\u3082\u767a\u58f2\u3055\u308c\u3066\u3044\u307e\u3059<\/a>\u3002<\/p>\n<p class=\"details\">\u3053\u306e\u8ad6\u6587\u3067\u7d39\u4ecb\u3055\u308c\u3066\u3044\u308b\u65b9\u6cd5\u306f\u3001\u300c\u67d3\u8272\u4f53\u3092\u6539\u5909\u3057\u305f\u9175\u6bcd\u300d\u3068\u300c\u63a5\u5408\u300d\u3068\u3044\u3046\u9175\u6bcd\u306a\u3089\u3067\u306f\u306e\u6280\u8853\u3092\u7528\u3044\u3066\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u79fb\u3057\u307e\u3059\u3002<\/p>\n<p class=\"details\">\u63a5\u5408\u3067\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u79fb\u3059\u5834\u5408\u306b\u306f\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u6301\u3063\u305f\u30c9\u30ca\u30fc\uff08\u4f9b\u4e0e\uff09\u682a\u3068\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5c0e\u5165\u3057\u305f\u3044\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\uff08\u53d7\u5bb9\uff09\u682a \u30fc \u901a\u5e38\u306f\u4f55\u3089\u304b\u306e\u5909\u7570\u682a \u30fc \u00a0\u3092\u63a5\u5408\u3055\u305b\u3001\u4e8c\u500d\u4f53\u3057\u3001\u305d\u306e\u5f8c\u4f55\u3089\u304b\u306e\u65b9\u6cd5\u3067\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u682a\u306e\u907a\u4f1d\u578b\u3092\u6301\u3064\u4e00\u500d\u4f53\u306b\u3082\u3069\u3059\u3068\u3044\u3046\u624b\u7d9a\u304d\u304c\u53d6\u3089\u308c\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<p class=\"details\">\u3053\u306e\u623b\u3059\u64cd\u4f5c\u306f\u3001\u666e\u901a\u306b\u8003\u3048\u308b\u3068\u80de\u5b50\u5f62\u6210 \u2192 \u4e00\u500d\u4f53\u682a\u306e\u9078\u5225\u3001\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u308b\u306e\u3067\u3059\u304c\u3001\u3053\u308c\u304c\u7d50\u69cb\u9762\u5012\u81ed\u3044\u3002\u3060\u3063\u305f\u3089\uff11\u3064\u305a\u3064\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5c0e\u5165\u3057\u305f\u307b\u3046\u304c\u65e9\u3044\u3002<\/p>\n<p class=\"details\">\u305d\u3053\u3067\u3053\u306e\u8ad6\u6587\u3067\u306f\u3068\u3093\u3067\u3082\u306a\u304f\u30b9\u30da\u30b7\u30e3\u30eb\u306a\u6280\u304c\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p class=\"details\">\u5404\u67d3\u8272\u4f53\u306b\u306f\u3001\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u3068\u3044\u3046\u90e8\u5206\u304c\u3042\u308a\u3001\u3053\u308c\u306f\u67d3\u8272\u4f53\u306e\u5206\u914d\u306b\u95a2\u308f\u308b\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u679c\u305f\u3057\u3066\u3044\u307e\u3059\u3002\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u304c\u58ca\u308c\u308b\u3068\u67d3\u8272\u4f53\u306f\u3046\u307e\u304f\u5206\u914d\u3055\u308c\u306a\u304f\u306a\u308b\u306e\u3067\u3001\u67d3\u8272\u4f53\u306e\u4fdd\u6301\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<p class=\"details\">\u51fa\u82bd\u9175\u6bcd\u306e\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u306f\u3068\u3066\u3082\u5c0f\u3055\u304f\u3001\u91cd\u8981\u306a\u90e8\u5206\u306f100bp\u7a0b\u5ea6\u3057\u304b\u3042\u308a\u307e\u305b\u3093\u3002\u3055\u3089\u306b\u3053\u306e\u8fd1\u508d\u306b\u975e\u5e38\u306b\u5f37\u529b\u306a\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u914d\u7f6e\u3057\u3066mRNA\u306e\u8ee2\u5199\u3092\u8a98\u5c0e\u3059\u308b\u3068\u3001\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u306b\u7d50\u5408\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7d50\u5408\u3067\u304d\u306a\u304f\u306a\u308a\u3001\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u306e\u6a5f\u80fd\u304c\u306a\u304f\u306a\u308a\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u305d\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u4f55\u3089\u304b\u306e\u6761\u4ef6\u3067\u306e\u307fON\u306b\u306a\u308b\u3088\u3046\u3059\u308c\u3070\u3001\u6761\u4ef6\u4f9d\u5b58\u7684\u306b\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u306e\u6a5f\u80fd\u3092\u5931\u308f\u305b\u3001\u76ee\u7684\u306e\u67d3\u8272\u4f53\u3092\u5931\u308f\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u308f\u3051\u3067\u3059\uff08\u3053\u308c\u3092 Conditional CEN \u3068\u547c\u3073\u307e\u3059\uff09\u3002<\/p>\n<p class=\"details\">\u00a0\u4eca\u56de\u306e\u5834\u5408\u3001\u30c9\u30ca\u30fc\u682a\u306e\u67d3\u8272\u4f5316\u672c\u3059\u3079\u3066\u306b Conditional CEN \u3092\u7d44\u307f\u8fbc\u3093\u3067\u304a\u304d\u3001\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u3068\u63a5\u5408\u3055\u305b\u4e8c\u500d\u4f53\u5316\u3055\u305b\u305f\u3042\u3068\u3001\u6761\u4ef6\u3092\u5909\u3048\u3066 Conditional CEN \u306e\u6a5f\u80fd\u3092\u5931\u308f\u305b\u3001\u30c9\u30ca\u30fc\u682a\u306e\u67d3\u8272\u4f53\u3092\u3059\u3079\u3066\u53d6\u308a\u9664\u3044\u3066\u3057\u307e\u3044\u4e00\u500d\u4f53\u306b\u623b\u3059\u306e\u3067\u3059\u3002<\/p>\n<p class=\"details\">\u3044\u3084\u301c\u3001\u3053\u308c\u3082\u9175\u6bcd\u306a\u3089\u3067\u306f\u306e\u3001\u3068\u3093\u3067\u3082\u306a\u3044\u30b9\u30b4\u6280\u3067\u3059\u306d\u3002<\/p>\n<p class=\"details\">\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Selective ploid&#8230;<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[10,25,23,8,9],"tags":[],"class_list":["post-3096","post","type-post","status-publish","format-standard","hentry","category-10","category-25","category-23","category-8","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3096","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3096"}],"version-history":[{"count":7,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3096\/revisions"}],"predecessor-version":[{"id":3103,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3096\/revisions\/3103"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3096"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3096"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3096"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}