{"id":3104,"date":"2015-09-16T15:57:43","date_gmt":"2015-09-16T06:57:43","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3104"},"modified":"2015-09-16T15:57:43","modified_gmt":"2015-09-16T06:57:43","slug":"%e3%83%9b%e3%83%ab%e3%83%a2%e3%83%b3%e3%82%92%e5%88%a9%e7%94%a8%e3%81%97%e3%81%9f%e6%96%b0%e3%81%97%e3%81%84%e9%81%ba%e4%bc%9d%e5%ad%90%e7%99%ba%e7%8f%be%e5%88%b6%e5%be%a1%e3%82%b7%e3%82%b9%e3%83%86","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3104","title":{"rendered":"\u30db\u30eb\u30e2\u30f3\u3092\u5229\u7528\u3057\u305f\u65b0\u3057\u3044\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u30b7\u30b9\u30c6\u30e0"},"content":{"rendered":"<p><a href=\"https:\/\/yeastcolloquium.wordpress.com\/\">\u9175\u6bcd\u30b3\u30ed\u30ad\u30a2\u30e0<\/a>\u306b\u4ee5\u524d\u79c1\u304c\u6295\u7a3f\u3057\u305f\u5185\u5bb9\u306b\u6539\u5909\u30fb\u8ffd\u8a18\u3057\u3066\u8f09\u305b\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21965290\">Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae.<\/a>\u00a0McIsaac RS, Silverman SJ, McClean MN, Gibney PA, Macinskas J, Hickman MJ, Petti A, Botstein D. Mol Biol Cell. 2011 Sep 30. PMID: 21965290<\/p>\n<p>David Botstein\u306e\u30b0\u30eb\u30fc\u30d7\u304c2011\u5e74\u306b\u767a\u8868\u3057\u305f\u8ad6\u6587\u3067\u3059\u3002\u9175\u6bcd\u3067\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092ON\/OFF\u3059\u308b\u306e\u306b\u6700\u3082\u3088\u304f\u4f7f\u308f\u308c\u308b\u306e\u304cGAL promoter\u3067\u3001\u305d\u306e\u6b21\u304cMET promoter\u3067\u3057\u3087\u3046\u304b\uff1f\u3053\u308c\u3089\u306f\u78ba\u304b\u306bON\/OFF\u304c\u306f\u3063\u304d\u308a\u3057\u3066\u53cd\u5fdc\u3082\u65e9\u3044\u3068\u3044\u3046\u8a55\u5224\u306a\u306e\u3067\u3059\u304c\u3001\u300c\u6804\u990a\u6761\u4ef6\u3092\u304b\u3048\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u300d\u3068\u3044\u3046\u6700\u5927\u306e\u30c7\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u4eca\u56de\u767a\u8868\u3055\u308c\u305f\u306e\u306f\u3001\u30a8\u30b9\u30c8\u30ed\u30b2\u30f3\u30ec\u30bb\u30d7\u30bf\u30fc\u306e\u6838\u30fc\u7d30\u80de\u8cea\u79fb\u884c\u306e\u5236\u5fa1\u3092\u30a8\u30b9\u30c8\u30ed\u30b2\u30f3\uff08\u03b2-setradiol\uff09\u3067\u5236\u5fa1\u3059\u308b\u3068\u3044\u3046\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u8ee2\u5199\u56e0\u5b50\u306e\u69cb\u9020\u304c\u3001GAL4DB-Estrogen receptor-VP16AD\u3067\u3042\u308b\u4e8b\u304b\u3089GEV\u3068\u547c\u3070\u308c\u3066\u3044\u307e\u3059\u3002\u30db\u30eb\u30e2\u30f3\u3092\u52a0\u3048\u3066\u308f\u305a\u304b\uff15\u5206\u3067\u8ee2\u5199\u6d3b\u6027\u5316\u304c\u59cb\u307e\u308b\u3068\u3044\u3046\u300c\u8fc5\u901f\u306a\u300d\u30b7\u30b9\u30c6\u30e0\u3001\u307e\u305f\u3001\u3053\u306eGEV-\u30a8\u30b9\u30c8\u30ed\u30b2\u30f3\u306b\u3088\u3063\u3066\u3001GAL\u907a\u4f1d\u5b50\u7fa4\u3092\u542b\u3080\u308f\u305a\u304b\u306a\u907a\u4f1d\u5b50\u3057\u304b\u5f71\u97ff\u3092\u53d7\u3051\u306a\u3044\u3068\u306e\u3053\u3068\u3067\u3059\u3002<\/p>\n<p>GEV\u306e\u826f\u3044\u3068\u3053\u308d\u306f\u3001GAL1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3044\u3046\u4eca\u307e\u3067\u306b\u975e\u5e38\u306b\u3088\u304f\u4f7f\u308f\u308c\u3066\u304d\u305f\u30ea\u30bd\u30fc\u30b9\u304c\u305d\u306e\u307e\u307e\u4f7f\u3048\u308b\u3068\u3044\u3046\u4e8b\u3067\u3059\u3002\u3053\u306e\u30db\u30eb\u30e2\u30f3\u306b\u3088\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u30b7\u30b9\u30c6\u30e0\u3001\u3053\u308c\u304b\u3089\u5e83\u307e\u3063\u3066\u3044\u304f\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n<p>\u6c17\u306b\u306a\u308b\u3053\u306e\u30db\u30eb\u30e2\u30f3\u306e\u304a\u5024\u6bb5\u3067\u3059\u304c\u3001<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/ProductDetail.do?D7=0&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;N4=E8875|SIGMA&amp;N25=0&amp;QS=ON&amp;F=SPEC\">Sigma-Aldrich\u3067\u8abf\u3079\u308b\u3068<\/a>250mg\u30675,200\u5186\u3002\u8ad6\u6587\u306b\u3088\u308b\u306810nM\u3067\u6bd2\u6027\u3082\u306a\u304f\u3046\u307e\u304f\u4f7f\u3048\u308b\u3068\u3044\u3046\u4e8b\u3067\u8a08\u7b97\u3057\u3066\u307f\u308b\u3068\u300110nM\u306e\u6eb6\u6db21L\u3042\u305f\u308a\u30fb\u30fb\u30fb5\u92ad\uff01\uff01\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\uff082%\u3068\u3057\u30661L\u3042\u305f\u308a\u7d04100\u5186\uff09\u3088\u308a\u305a\u3063\u3068\u5b89\u3044\uff01\uff1f\u3000\u305f\u3060\u5148\u65e5\u306e\u9175\u6bcd\u306e\u5b66\u4f1a\u3067\u3082\u7814\u7a76\u8005\u9593\u3067\u8a71\u984c\u306b\u306a\u3063\u305f\u306e\u3067\u3059\u304c\u3001\u3053\u308c\u5973\u6027\u30db\u30eb\u30e2\u30f3\u306e\u8a98\u5c0e\u4f53\u306a\u306e\u3067\u3001\u4eba\u4f53\u306b\u60aa\u5f71\u97ff\u304c\u3042\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305d\u306e\u3046\u3061\u7537\u6027\u306e\u9175\u6bcd\u7814\u7a76\u8005\u304c\u307f\u3093\u306a\u5973\u6027\u3063\u307d\u304f\u306a\u3063\u305f\u308a\u3057\u3066\u30fb\u30fb\u30fb\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u4ee5\u4e0b\u306f\u8ffd\u8a18\u3067\u3059\u3002<\/p>\n<p>\u3061\u306a\u307f\u306b\u3053\u306e\u5b9f\u9a13\u7cfb\u306f\u3055\u3089\u306a\u308b\u6539\u826f\u7248\u304c\u4f5c\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23275543\">Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast.<\/a>\u00a0McIsaac RS, Oakes BL, Wang X, Dummit KA, Botstein D, Noyes MB.\u00a0<span class=\"jrnl\" title=\"Nucleic acids research\">Nucleic Acids Res<\/span>. 2013 Feb 1;41(4):e57. doi: 10.1093\/nar\/gks1313. Epub 2012 Dec 28.\u00a0PMID:23275543<\/p>\n<p>\u3053\u3061\u3089\u3067\u306f\u3055\u3089\u306b\u4eba\u5de5\u8ee2\u5199\u56e0\u5b50\u3084\u7d50\u5408\u914d\u5217\u306e\u6539\u826f\u3092\u884c\u3063\u3066\u3001\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u4ee5\u5916\u306b\u306f\u307e\u3063\u305f\u304f\u5f71\u97ff\u3092\u4e0e\u3048\u306a\u3044\u3068\u8b33\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u5b9f\u969b\u3001\u79c1\u3082\u3053\u306e\u5b9f\u9a13\u7cfb\u3092\u5165\u624b\u3057\u3066\u4f7f\u3063\u3066\u307f\u307e\u3057\u305f\uff08\u8ad6\u6587\u306b\u66f8\u3044\u3066\u3042\u308b\u3068\u304a\u308a\u306b\u306f\u3044\u304b\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u306f\u3088\u304f\u3042\u308b\u3053\u3068\u3067\u3059\u306e\u3067\uff09\u3002\u3059\u308b\u3068\u78ba\u304b\u306b GAL\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u540c\u3058\u304f\u3001\u975e\u8a98\u5c0e\u6761\u4ef6\u3067\u306e\u767a\u73fe\u306f\u691c\u51fa\u3055\u308c\u306a\u3044\u30ec\u30d9\u30eb\u3067\u3001\u8a98\u5c0e\u6761\u4ef6\u3067\u306e\u767a\u73fe\u306fGAL\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e6\u500d\u7a0b\u5ea6\u3042\u308b\u3068\u3044\u3046\u3001\u304b\u306a\u308a\u512a\u308c\u305f\u30b7\u30b9\u30c6\u30e0\u3067\u3042\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u306e\u5b9f\u9a13\u7cfb\u306e\u6700\u5927\u306e\u30c7\u30e1\u30ea\u30c3\u30c8\u306f\u3001\u4eba\u5de5\u8ee2\u5199\u56e0\u5b50\u3092\u9175\u6bcd\u306b\u6301\u305f\u305b\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u3053\u3068\u3067\u3059\u306d\u3002\u8272\u3005\u306a\u682a\u3067\u89e3\u6790\u3057\u305f\u3044\u5834\u5408\u306b\u3001\u3044\u3061\u3044\u3061\u4eba\u5de5\u8ee2\u5199\u56e0\u5b50\u3092\u30b2\u30ce\u30e0\u306b\u6301\u305f\u3059\u306e\u306f\u5c11\u3057\u5384\u4ecb\u3067\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9175\u6bcd\u30b3\u30ed\u30ad\u30a2\u30e0\u306b\u4ee5\u524d\u79c1\u304c\u6295\u7a3f\u3057&#8230;<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[10,25,133,8,9],"tags":[],"class_list":["post-3104","post","type-post","status-publish","format-standard","hentry","category-10","category-25","category-133","category-8","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3104","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3104"}],"version-history":[{"count":3,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3104\/revisions"}],"predecessor-version":[{"id":3107,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3104\/revisions\/3107"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3104"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3104"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3104"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}