{"id":3134,"date":"2015-12-29T15:52:51","date_gmt":"2015-12-29T06:52:51","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3134"},"modified":"2017-08-09T20:59:56","modified_gmt":"2017-08-09T11:59:56","slug":"%e8%b5%a4%e8%89%b2%e8%9b%8d%e5%85%89%e3%82%bf%e3%83%b3%e3%83%91%e3%82%af%e8%b3%aa-yemrfpmcherry-%e3%81%ae%e5%88%87%e6%96%ad%e3%81%ae%e8%ac%8e%e3%81%8c%e8%a7%a3%e6%b1%ba%e3%81%97%e3%81%9f","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3134","title":{"rendered":"\u300c\u8d64\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea yEmRFP\/mCherry \u304c\u5207\u308c\u3061\u3083\u3046\u8b0e\u300d\u304c\u89e3\u6c7a\u3057\u305f"},"content":{"rendered":"<p>\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u4eca\u306e\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u306b\u306f\u306a\u304f\u3066\u306f\u306a\u3089\u306a\u3044\u91cd\u8981\u306a\u30c4\u30fc\u30eb\u3067\u3059\u3002\u4e00\u756a\u521d\u3081\u306b\u898b\u3064\u304b\u3063\u305f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u4e0b\u6751\u8129\u5148\u751f\u304c\u767a\u898b\u3057\u305f\u30af\u30e9\u30b2\u306e\u7dd1\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08GFP\u3001\u56f31\uff09\u3067\u3001\u69d8\u3005\u306a\u7814\u7a76\u306b\u4f7f\u308f\u308c\u3066\u3044\u308b\u306e\u306f\u3054\u5b58\u77e5\u306e\u3053\u3068\u304b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<figure id=\"attachment_3135\" aria-describedby=\"caption-attachment-3135\" style=\"width: 255px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3683.jpg\"><img fetchpriority=\"high\" decoding=\"async\" class=\"size-medium wp-image-3135\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3683-255x300.jpg\" alt=\"3D printed GFP\" width=\"255\" height=\"300\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3683-255x300.jpg 255w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3683-871x1024.jpg 871w\" sizes=\"(max-width: 255px) 100vw, 255px\" \/><\/a><figcaption id=\"caption-attachment-3135\" class=\"wp-caption-text\">\u56f31. <a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3008\" target=\"_blank\" rel=\"noopener\">3D\u30d7\u30ea\u30f3\u30c8<\/a>\u3057\u305fGFP\u3002\u672c\u30a8\u30f3\u30c8\u30ea\u30fc\u3068\u306f\u76f4\u63a5\u95a2\u4fc2\u306a\u3044\u304c\u3001\u304b\u308f\u3044\u3044\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>GFP\u306b\u3064\u3065\u3044\u3066\u69d8\u3005\u306a\u7279\u6027\u3092\u6301\u3063\u305f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7d9a\u3005\u3068\u767a\u898b\u30fb\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3044\u308d\u3044\u308d\u306a\u30ab\u30e9\u30fc\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u305d\u306e\u4e00\u3064\u3067\u3059\u304c\u3001\u540c\u3058\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u8a00\u3063\u3066\u3082\u3060\u3044\u3076\u7279\u6027\u304c\u9055\u3063\u305f\u308a\u3059\u308b\u306e\u3067\u3001GFP\u3068\u540c\u3058\u3064\u3082\u308a\u3067\u6271\u3046\u3068\u5b9f\u9a13\u304c\u601d\u3063\u305f\u3088\u3046\u306b\u884c\u304b\u306a\u3044\u3053\u3068\u3082\u3042\u3063\u305f\u308a\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u300c<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23149748\" target=\"_blank\" rel=\"noopener\">\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u3063\u3066\u7d30\u80de\u6bd2\u6027\u304c\u9055\u3046\u3088<\/a>\u300d\u3001\u3068\u3044\u3063\u305f\u8ad6\u6587\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306f<a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=569\" target=\"_blank\" rel=\"noopener\">GFP\u3092\u9175\u6bcd\u304c\u6b7b\u306c\u307b\u3069\u767a\u73fe\u3055\u305b\u3066\u307f\u308b\u3068\u3044\u3063\u305f\u5b9f\u9a13<\/a>\u3092\u884c\u3063\u3066\u3044\u308b\u306e\u3067\u3059\u304c\u3001\u8d64\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08yEmRFP\/mCherry*\uff09\u3092\u4f7f\u3063\u3066\u3082\u540c\u69d8\u306a\u5b9f\u9a13\u3092\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>*yEmRFP\u306f\u3001\u9175\u6bcd\u306e\u30b3\u30c9\u30f3\u306b\u6700\u9069\u5316\u3057\u305fmCherry\u3067\u3059\u3002yEmRFP\u3068mCherry\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u306f\u540c\u3058\u3082\u306e\u3067\u3059\u3002<\/p>\n<p>\u3055\u3066\u3001\u3042\u308b\u6642\u3001yEmRFP\u3092\u5927\u91cf\u767a\u73fe\u3057\u3066\u3044\u308b\u9175\u6bcd\u306e\u5168\u30bf\u30f3\u30d1\u30af\u8cea\u3092LDS-PAGE\u3067\u96fb\u6c17\u6cf3\u52d5\u3057\u3066\u307f\u307e\u3057\u305f\u3002\u3059\u308b\u3068\u3001yEmRFP\uff0826.7kDa\uff09\u3068\u540c\u3058\u30b5\u30a4\u30ba\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u52a0\u3048\u3066\u3001\u30b5\u30a4\u30ba\u306e\u5c0f\u3055\u306a\u30d0\u30f3\u30c9\u304c2\u672c\u898b\u3048\u307e\u3057\u305f\uff08\u56f32\u3001\u7d2b\u8272\u306e\u77e2\u5370\uff09\u3002<\/p>\n<figure id=\"attachment_3136\" aria-describedby=\"caption-attachment-3136\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/3f82f308b956ab57c7bedced9a7e044f.png\"><img decoding=\"async\" class=\"size-large wp-image-3136\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/3f82f308b956ab57c7bedced9a7e044f-881x1024.png\" alt=\"Electrophoresis of yEmRFP\/mCherry \" width=\"580\" height=\"674\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/3f82f308b956ab57c7bedced9a7e044f-881x1024.png 881w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/3f82f308b956ab57c7bedced9a7e044f-258x300.png 258w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/3f82f308b956ab57c7bedced9a7e044f.png 1078w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3136\" class=\"wp-caption-text\">\u56f32. yEmRFP\u306e\u96fb\u6c17\u6cf3\u52d5\u3002\u5207\u65ad\u3055\u308c\u305f\u5c0f\u3055\u306a\u30d0\u30f3\u30c9\uff08\u7d2b\u8272\u306e\u77e2\u5370\uff09\u304c\u898b\u3048\u308b\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u3053\u308c\u3089\u306e\u30b5\u30a4\u30ba\u3092\u8db3\u3059\u3068\u3061\u3087\u3046\u3069yEmRFP\u3068\u540c\u3058\u306b\u306a\u308b\u306e\u3067\u3001\u4e0b\u306e\uff12\u672c\u306e\u30d0\u30f3\u30c9\u5207\u65ad\u3055\u308c\u305fyEmRFP\u3067\u306f\u306a\u3044\u304b\u3068\u601d\u3044\u307e\u3057\u305f\u3002\u9175\u6bcd\u306e\u7814\u7a76\u8005\u30e1\u30fc\u30ea\u30f3\u30b0\u30ea\u30b9\u30c8\u3067\u554f\u3044\u5408\u308f\u305b\u305f\u6240\u3001yEmRFP\u304c\u5207\u308c\u308b\u3053\u3068\u3092\u3054\u5b58\u77e5\u3067\u3001\u3060\u3051\u3069\u3069\u3053\u3067\u5207\u308c\u308b\u306e\u304b\u3001\u305d\u308c\u3092\u3069\u3046\u3057\u305f\u3089\u89e3\u6c7a\u3067\u304d\u308b\u306e\u304b\u304c\u308f\u304b\u3089\u306a\u3044\u3068\u3044\u3046\u7814\u7a76\u8005\u306e\u65b9\u304c\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u305d\u308c\u306a\u3089\u81ea\u5206\u305f\u3061\u3067\u5207\u308c\u3066\u3044\u308b\u3068\u3053\u308d\u3092\u7279\u5b9a\u3057\u3066\u307f\u3088\u3046\u3001\u3068\u3044\u3046\u3053\u3068\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8cea\u91cf\u5206\u6790\u306e\u5c02\u9580\u5bb6\u3067\u3042\u308b\u660e\u6cbb\u5927\u5b66\u8fb2\u5b66\u90e8\u306e\u7d00\u85e4\u572d\u6cbb\u5148\u751f\u306b\u304a\u9858\u3044\u3057\u3066\u3001\u4e0a\u8a18\u306eBand1\u3068Band2\u306e\u30a2\u30df\u30ce\u9178\u914d\u5217\u306e\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u7d50\u679c\u306e\u8a73\u7d30\u306f\u3053\u3053\u3067\u306f\u5272\u611b\u3057\u307e\u3059\u304c\u3001\u56f33\u306e\u7070\u8272\u306e\u7dda\u306e\u4e2d\u306e\u3069\u3053\u304b\u3067\u5207\u308c\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u307e\u3067\u306f\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u5834\u6240\u3092\u306f\u3063\u304d\u308a\u3068\u7279\u5b9a\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n<figure id=\"attachment_3137\" aria-describedby=\"caption-attachment-3137\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/17bcede201fee9ad99dbb45193512d2a.png\"><img decoding=\"async\" class=\"size-large wp-image-3137\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/17bcede201fee9ad99dbb45193512d2a-1024x714.png\" alt=\"Structure of yEmRFP\" width=\"580\" height=\"404\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/17bcede201fee9ad99dbb45193512d2a-1024x714.png 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/17bcede201fee9ad99dbb45193512d2a-300x209.png 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/17bcede201fee9ad99dbb45193512d2a.png 1620w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3137\" class=\"wp-caption-text\">\u56f33. yEmRFP\u306e1\u6b21\u69cb\u9020\u3002\u8cea\u91cf\u5206\u6790\u306e\u7d50\u679c\u3001\u9ed2\u7dda\u306e\u3069\u3053\u304b\u3067\u5207\u308c\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u307e\u3067\u306f\u308f\u304b\u3063\u305f\u3002\u8d64\u3067\u56f2\u307e\u308c\u305f\u90e8\u5206\u304c\u767a\u8272\u56e3\u3092\u5f62\u6210\u3059\u308b\u30a2\u30df\u30ce\u9178\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u305d\u308c\u304c\u6628\u5e742014\u5e74\u306e4\u6708\u9803\u3002\u3053\u308c\u4ee5\u4e0a\u6df1\u5165\u308a\u3059\u308b\u306e\u306f\u96e3\u3057\u3044\u3068\u8003\u3048\u3001\u3053\u306e\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\uff08\uff1f\uff09\u306f\u4e00\u7aef\u30da\u30f3\u30c7\u30a3\u30f3\u30b0\u3068\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u305d\u308c\u304c\u307e\u305f\u52d5\u304d\u51fa\u3057\u305f\u306e\u306f\u3001\u3072\u3087\u3093\u306a\u304d\u3063\u304b\u3051\u304b\u3089\u3067\u3059\u3002<\/p>\n<p>\u4eca\u5e742015\u5e74\u306e11\u6708\u306b\u30b7\u30f3\u30ac\u30dd\u30fc\u30eb\u3067\u958b\u304b\u308c\u305f\u5b66\u4f1a\u306b\u53c2\u52a0\u3057\u305f\u6642\u306b\u3001\u77e5\u4eba\u306b\u7d39\u4ecb\u3057\u3066\u3044\u305f\u3060\u3044\u305f<a href=\"http:\/\/www.waseda.jp\/WABIOS\/jp\/\" target=\"_blank\" rel=\"noopener\">\u65e9\u7a32\u7530\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\u30b7\u30f3\u30ac\u30dd\u30fc\u30eb\u7814\u7a76\u6240\uff08WABIOS\uff09<\/a>\u306e\u5317\u53e3\u54f2\u4e5f\u5148\u751f\u306e\u7814\u7a76\u5ba4\u3092\u8a2a\u306d\u3001\u5317\u53e3\u5148\u751f\u306e\u7814\u7a76\u306b\u3064\u3044\u3066\u304a\u8a71\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002\u5317\u53e3\u5148\u751f\u306f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c02\u9580\u5bb6\u3067\u3057\u305f\u3002<\/p>\n<p>\u305d\u308c\u3067\u5317\u53e3\u5148\u751f\u306a\u3089\u4f55\u304b\u3054\u5b58\u77e5\u304b\u3082\u3057\u308c\u306a\u3044\u3068\u601d\u3044\u3001\u300cyEmRFP\u304c\u5207\u308c\u308b\u8b0e\u300d\u306b\u3064\u3044\u3066\u304a\u5c0b\u306d\u3057\u305f\u308f\u3051\u3067\u3059\u3002\u5317\u53e3\u5148\u751f\u3082\u5207\u308c\u308b\u3053\u3068\u306f\u3054\u5b58\u77e5\u3067\u3057\u305f\u304c\u3001\u3069\u3053\u304c\u5207\u308c\u308b\u304b\u306f\u3054\u5b58\u77e5\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n<p>\u305f\u3060\u3001\u300c\uff08\u5c02\u9580\u5bb6\u306a\u306e\u3067\uff09\u8cea\u91cf\u5206\u6790\u306e\u7d50\u679c\u3092\u898b\u308c\u3070\u3069\u3053\u304b\u7279\u5b9a\u3067\u304d\u308b\u304b\u3082\u3057\u308c\u306a\u3044\u300d\u3068\u304a\u3063\u3057\u3083\u3063\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002\u3068\u3044\u3046\u3053\u3068\u3067\u3001\u5e30\u56fd\u3057\u3066\u8cea\u91cf\u5206\u6790\u306e\u7d50\u679c\u3092\u304a\u9001\u308a\u3057\u3001\u3069\u3053\u304c\u5207\u308c\u3066\u3044\u308b\u304b\u306e\u4e88\u60f3\u3092\u305f\u3066\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u5317\u53e3\u5148\u751f\u306e\u4e88\u60f3\u306f\u3001\u300c\u767a\u8272\u56e3\u306e\u8fd1\u304f\u3067\u5207\u308c\u3066\u3044\u308b\u300d\u3068\u3044\u3046\u3082\u306e\u3067\u3057\u305f\u3002\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u56f33\u306e\u8d64\u8272\u3067\u56f2\u3093\u3060\u30a2\u30df\u30ce\u9178\u3069\u3046\u3057\u304c\u5316\u5b66\u7d50\u5408\u3092\u8d77\u3053\u3057\u3066\u767a\u8272\u56e3\u3092\u81ea\u5f8b\u7684\u306b\u5f62\u6210\u3059\u308b\u7279\u5fb4\u304c\u3042\u308a\u307e\u3059\u3002\u3042\u308b\u7a2e\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u306f\u3001\u3053\u308c\u306b\u5f15\u304d\u7d9a\u3044\u3066\u30a2\u30df\u30ce\u9178\u5185\u90e8\u306e\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u304c\u5207\u308c\u308b\u3089\u3057\u3044\u306e\u3067\u3059\uff08\u56f34\uff09\u3002yEmRFP\u306e\u5207\u65ad\u3082\u304d\u3063\u3068\u3053\u308c\u3060\u308d\u3046\u3068\u3044\u3046\u3053\u3068\u3067\u3057\u305f\u3002\u3055\u3059\u304c\u5c02\u9580\u5bb6\u3001\u3042\u3063\u3068\u3044\u3046\u9593\u306b\u5834\u6240\u3092\u7279\u5b9a\u3067\u3059\u3002<\/p>\n<figure id=\"attachment_3138\" aria-describedby=\"caption-attachment-3138\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/F6_large.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-3138\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/F6_large-1024x963.jpg\" alt=\"Fluorophore \" width=\"580\" height=\"545\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/F6_large-1024x963.jpg 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/F6_large-300x282.jpg 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/F6_large.jpg 1280w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3138\" class=\"wp-caption-text\">\u56f34. \u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u8272\u56e3\u306e\u5f62\u6210\u3002\u30a2\u30df\u30ce\u9178\u306e\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u304c\u5207\u308c\u308b\u3082\u306e\u3082\u3042\u308b\u3002<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20664080\" target=\"_blank\" rel=\"noopener\">Chudakov 2010<\/a>\u3088\u308a\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u3067\u3001\u3058\u3083\u3042\u3069\u3046\u3057\u305f\u3089\u5207\u308c\u306a\u304f\u306a\u308b\u304b\u3001\u3068\u3044\u3046\u3053\u3068\u306a\u306e\u3067\u3059\u304c\u3001\u5317\u53e3\u5148\u751f\u306e\u6700\u7d42\u7684\u306a\u52a9\u8a00\u306f\u3001\u300c\u767a\u8272\u56e3\u306e\u30a2\u30df\u30ce\u9178\u306eY\u3092G\u306b\u5909\u3048\u308c\u3070\u3001\u767a\u8272\u56e3\u304c\u5f62\u6210\u3055\u308c\u306a\u304f\u306a\u308a\u304d\u308c\u306a\u304f\u306a\u308b\u3060\u308d\u3046\uff08\uff11\uff09\u3002\u305d\u308c\u3067\u3082\u5207\u308c\u305f\u3089\u5225\u306e\u3068\u3053\u308d\u304c\u5207\u308c\u3066\u3044\u308b\uff08\uff12\uff09\u3002\u300d\u3068\u3044\u3046\u3082\u306e\u3067\u3057\u305f\u3002<\/p>\n<p>\u3082\u3057\uff11\u3060\u3063\u305f\u3089\uff1a\u5207\u308c\u308b\u8b0e\u306f\u89e3\u3051\u308b\u304b\u3089\u5b09\u3057\u3044\u3093\u3060\u3051\u3069\u3001\u86cd\u5149\u3092\u6301\u305f\u306a\u304f\u306a\u3063\u3061\u3083\u3046\u306e\u3067RFP\u3068\u3057\u3066\u306f\u4f7f\u3048\u306a\u3044\u306e\u3067\u60b2\u3057\u3044\u3002<\/p>\n<p>\u3082\u3057\uff12\u3060\u3063\u305f\u3089\uff1a\u307e\u3060\u8b0e\u306f\u89e3\u3051\u306a\u3044\u3051\u3069\u3001\u5207\u65ad\u3055\u308c\u306a\u3044RFP\u3092\u4f5c\u308c\u308b\u304b\u3082\u3057\u308c\u306a\u3044\u306e\u3067\u5b09\u3057\u3044\u3002<\/p>\n<p>\u3068\u3044\u3046\u4e8b\u306b\u306a\u308a\u307e\u3059\u3002\u3068\u3044\u3046\u3053\u3068\u3067\u3001Y\u3092G\u306b\u5909\u3048\u305f\u5909\u7570\uff08Y72G\uff09\u3092\u4f5c\u3063\u3066\u307f\u307e\u3057\u305f\u3002\u56f35\u306f\u9175\u6bcd\u7d30\u80de\u3067\u3059\u3002yEmRFP\u3092\u305f\u304f\u3055\u3093\u4f5c\u3063\u3066\u3044\u308b\u9175\u6bcd\uff08\u5de6\u304b\u3089\u4e8c\u756a\u76ee\uff09\u306f\u30d4\u30f3\u30af\u8272\u306a\u306e\u3067\u3059\u304c\u3001Y72G\u3092\u4f5c\u3063\u3066\u3044\u308b\u9175\u6bcd\uff08\u4e00\u756a\u53f3\uff09\u3067\u306f\u8272\u304c\u3064\u304b\u306a\u304f\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<figure id=\"attachment_3139\" aria-describedby=\"caption-attachment-3139\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3680.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-3139\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3680-956x1024.jpg\" alt=\"yEmRFP mutants\" width=\"580\" height=\"621\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3680-956x1024.jpg 956w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3680-280x300.jpg 280w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/IMG_3680.jpg 1939w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3139\" class=\"wp-caption-text\">\u56f35. yEmRFP\u3092\u767a\u73fe\u3057\u3066\u3044\u308b\u9175\u6bcd\u3002 \u5de6\u304b\u3089\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08yEmRFP\u3092\u767a\u73fe\u3057\u3066\u3044\u306a\u3044\u9175\u6bcd\uff09\u3001yEmRFP\u3092\u767a\u73fe\u3057\u3066\u3044\u308b\u9175\u6bcd\u3001Q71M\u5909\u7570\uff08\u4eca\u56de\u306f\u95a2\u4fc2\u306a\u3044\uff09\u3001Y72G\u5909\u7570\u3092\u767a\u73fe\u3057\u3066\u3044\u308b\u9175\u6bcd\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u305d\u3057\u3066\u3001\u30c9\u30ad\u30c9\u30ad\u3057\u306a\u304c\u3089\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u96fb\u6c17\u6cf3\u52d5\u3057\u3066\u307f\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u306f\u30fb\u30fb\u30fb\u3002<\/p>\n<figure id=\"attachment_3140\" aria-describedby=\"caption-attachment-3140\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/ae4ba3ffd4e614e88421cb93d4b61a1b.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-3140\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/ae4ba3ffd4e614e88421cb93d4b61a1b-770x1024.png\" alt=\"yEmRFP\u3068\u305d\u306e\u5909\u7570\u4f53\u3092\u5927\u91cf\u306b\u767a\u73fe\u3057\u3066\u3044\u308b\u9175\u6bcd\u7d30\u80de\u306e\u5168\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u89e3\u6790\u3057\u305f\u3002Y72G\u5909\u7570\u3067\u306f\u5207\u65ad\u3055\u308c\u305f\u30d0\u30f3\u30c9\uff08\u7d2b\u8272\u77e2\u5370\uff09\u304c\u898b\u3048\u306a\u304f\u306a\u3063\u3066\u3044\u308b\u3002\" width=\"580\" height=\"771\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/ae4ba3ffd4e614e88421cb93d4b61a1b-770x1024.png 770w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/ae4ba3ffd4e614e88421cb93d4b61a1b-226x300.png 226w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/ae4ba3ffd4e614e88421cb93d4b61a1b.png 884w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3140\" class=\"wp-caption-text\">\u56f36. yEmRFP\u5909\u7570\u4f53\u306e\u89e3\u6790\u3002yEmRFP\u3068\u305d\u306e\u5909\u7570\u4f53\u3092\u5927\u91cf\u306b\u767a\u73fe\u3057\u3066\u3044\u308b\u9175\u6bcd\u7d30\u80de\u306e\u5168\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u89e3\u6790\u3057\u305f\u3002Y72G\u5909\u7570\u3067\u306f\u5207\u65ad\u3055\u308c\u305f\u30d0\u30f3\u30c9\uff08\u7d2b\u8272\u77e2\u5370\uff09\u304c\u898b\u3048\u306a\u304f\u306a\u3063\u3066\u3044\u308b\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u5207\u308c\u306a\u304f\u306a\u308a\u307e\u3057\u305f\u301c\uff08\u56f36\u306eY72G\u3067\u3059\uff09\u3002<\/p>\n<p>\u3068\u3044\u3046\u3053\u3068\u3067\u3001\u5317\u53e3\u5148\u751f\u304c\u8003\u3048\u305f\u3088\u3046\u306b\u767a\u8272\u56e3\u306e\u8fd1\u304f\u3067\u5207\u308c\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u308a\u305d\u3046\u3067\u3059\u3002\u6b8b\u5ff5\u30fb\u30fb\u30fb\u3060\u3051\u3069\u3068\u306b\u304b\u304f\u8b0e\u304c\u89e3\u3051\u305f\u3053\u3068\u306f\u826f\u304b\u3063\u305f\u3002\u8ad6\u6587\u306b\u3059\u308b\u7a0b\u306e\u7d50\u672b\u3067\u306f\u306a\u304b\u3063\u305f\u3068\u3044\u3046\u3053\u3068\u3067\u3001\u3053\u306e\u30d6\u30ed\u30b0\u306e\u30cd\u30bf\u306b\u3055\u305b\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u3061\u306a\u307f\u306b\u5317\u53e3\u5148\u751f\u306b\u3088\u308b\u3068\u3001\u300c\u3053\u306e\u767a\u8272\u56e3\u306e\u8fd1\u304f\u3067\u306e\u5207\u65ad\u3067\u306f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e3D\u69cb\u9020\u306f\u5d29\u308c\u306a\u3044\uff08\u7d30\u80de\u5185\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30d0\u30e9\u30d0\u30e9\u306b\u306a\u308b\u3053\u3068\u306f\u306a\u3044\uff09\u306e\u3067\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u5229\u7528\u3059\u308b\u5206\u306b\u306f\u305f\u3044\u3066\u3044\u554f\u984c\u306a\u3044\u300d\u3001\u3068\u306e\u3053\u3068\u3067\u3057\u305f\u3002<\/p>\n<p>\u5c02\u9580\u5bb6\u306b\u610f\u898b\u3092\u4f3a\u3046\u3053\u3068\u306f\u672c\u5f53\u306b\u5927\u4e8b\u3001\u3068\u3044\u3046\u30a8\u30f3\u30c8\u30ea\u30fc\u3067\u3057\u305f\u3002\u3054\u5354\u529b\u3044\u305f\u3060\u3044\u305f\u7d00\u85e4\u5148\u751f\u3001\u5317\u53e3\u5148\u751f\u3069\u3046\u3082\u3042\u308a\u304c\u3068\u3046\u3054\u3056\u3044\u307e\u3057\u305f\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u30fc\u30fc\u30fc\u30fc<\/p>\n<p>\u4f59\u8ac7\u3067\u3059\u304c\u30fb\u30fb\u30fb\u79c1\u306f\u4ee5\u524d\u5225\u306e\u76ee\u7684\u3067yEGFP\u306e\u767a\u8272\u56e3\u306e\u5909\u7570Y66G\u3092\u4f5c\u3063\u305f\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u56f32\u3067\u3082\u793a\u3057\u3066\u3044\u308b\u3088\u3046\u306b\u3001yEGFP\u306e\u5207\u308c\u305f\u30d0\u30f3\u30c8\u306f\u5168\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u89e3\u6790\u3067\u306f\u898b\u3048\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u56f37\u306e\u7d2b\u77e2\u5370\u306e\u3088\u3046\u306b\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u3067\u306f\u5207\u308c\u305f\u3068\u601d\u3057\u304d\u30d0\u30f3\u30c9\u304c<span style=\"font-size: 16px; line-height: 24px;\">\u5c11\u3057<\/span>\u898b\u3048\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u304c\u3001Y66G\u5909\u7570\u3067\u306f\u898b\u3048\u306a\u304f\u306a\u3063\u3066\u3044\u307e\u3059\u3002yEGFP\u3082\u308f\u305a\u304b\u3067\u306f\u3042\u308a\u307e\u3059\u304c\u3001\u767a\u8272\u56e3\u5f62\u6210\u5f8c\u306b\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u304c\u5207\u308c\u308b\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n<figure id=\"attachment_3141\" aria-describedby=\"caption-attachment-3141\" style=\"width: 447px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/feb79e1564771bc22daf8321406bf705.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-3141\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/feb79e1564771bc22daf8321406bf705.png\" alt=\"yEGFP-Y66G\u5909\u7570\u306e\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\" width=\"447\" height=\"647\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/feb79e1564771bc22daf8321406bf705.png 447w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2015\/12\/feb79e1564771bc22daf8321406bf705-207x300.png 207w\" sizes=\"(max-width: 447px) 100vw, 447px\" \/><\/a><figcaption id=\"caption-attachment-3141\" class=\"wp-caption-text\">\u56f37. yEGFP-Y66G\u5909\u7570\u306e\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u89e3\u6790\u3002yEGFP\u3082\u5c11\u3057\u306f\u5207\u308c\u3066\u3044\u308b\u306e\u304b\u3082\u3057\u308c\u306a\u3044\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u30fc\u30fc\u30fc\u30fc<\/p>\n<p>20160120\u8ffd\u8a18<\/p>\n<p class=\"p1\"><span class=\"s1\">\u3053\u306e\u5f8c\u3082\u3044\u308d\u3044\u308d\u8abf\u3079\u3066\u307f\u305f\u306e\u3067\u3059\u304c\u3001<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16594705\" target=\"_blank\" rel=\"noopener\">\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08\u306e\u5909\u7570\u4f53\u306a\u3069\uff09\u304c\u767a\u8272\u56e3\u3042\u305f\u308a\u3067\u5207\u308c\u308b\u73fe\u8c61\u306f\u3088\u304f\u89b3\u5bdf\u3055\u308c\u3066\u3044\u308b<\/a>\u3088\u3046\u3067\u3059\u3002<\/span><span class=\"s1\">\u307e\u305f\u3001<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11050230\" target=\"_blank\" rel=\"noopener\">\u300cRFP\uff08dsRed\uff09\u306f\u30dc\u30a4\u30eb\u3057\u305f\u3089\u306f\u767a\u8272\u56e3\u3042\u305f\u308a\u3067\u5207\u308c\u308b\u300d\u3068\u3044\u3046\u306e\u304c\u521d\u671f\u306e\u7814\u7a76\u3067\u308f\u304b\u3063\u3066\u3044\u305f<\/a>\u3088\u3046\u3067\u3059\u3002<\/span><span class=\"s1\">\u3067\u3059\u306e\u3067\u3001RFP\u306f <em>in vivo<\/em>\uff08in yeast\uff09\u3067\u306f\u5207\u308c\u3066\u3044\u306a\u304f\u3066\u3001SDS-PAGE\u3067\u89e3\u6790\u3059\u308b\u6642\u306b\u5207\u308c\u3066\u3044\u308b\u3088\u3046\u3067\u3059\u3002<\/span><\/p>\n<p class=\"p1\">\u300c\u5b9f\u9a13\u3092\u59cb\u3081\u308b\u524d\u306b\u6587\u732e\u3092\u3057\u3063\u304b\u308a\u8abf\u3079\u308d\u300d\u3068\u3044\u3046\u3053\u3068\u3067\u306f\u3042\u308b\u306e\u3067\u3059\u304c\u3001\u79d1\u5b66\u7814\u7a76\u304c\u5927\u304d\u304f\u5e83\u304c\u3063\u3066\u3044\u308b\u73fe\u4ee3\u3067\u306f\u306a\u304b\u306a\u304b\u3059\u3079\u3066\u3092\u8abf\u67fb\u3059\u308b\u3053\u3068\u306f\u96e3\u3057\u3044\u73fe\u5b9f\u3082\u3042\u308a\u307e\u3059\u3002\u5c02\u9580\u5bb6\u306e\u610f\u898b\u3092\u805e\u3044\u305f\u308a\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\u3092\u3057\u3066\u307f\u305f\u308a\u3057\u306a\u304c\u3089\u904e\u53bb\u306e\u77e5\u898b\u3092\u81ea\u5206\u305f\u3061\u306e\u77e5\u8b58\u3068\u3057\u3066\u84c4\u3048\u3066\u3044\u304f\u3068\u3044\u3046\u904e\u7a0b\u3082\u5fc5\u8981\u304b\u3068\u601d\u3044\u307e\u3059\uff08\u8a00\u3044\u8a33\u3067\u306f\u3042\u308a\u307e\u3059\u304c\uff09\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u4eca\u306e\u5206\u5b50\u751f\u7269\u5b66&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3140,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[25,9],"tags":[],"class_list":["post-3134","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-25","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3134","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3134"}],"version-history":[{"count":11,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3134\/revisions"}],"predecessor-version":[{"id":3434,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3134\/revisions\/3434"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3140"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3134"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3134"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}