{"id":3277,"date":"2016-12-20T16:53:31","date_gmt":"2016-12-20T07:53:31","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3277"},"modified":"2017-08-09T20:48:35","modified_gmt":"2017-08-09T11:48:35","slug":"%e3%83%96%e3%83%a9%e3%83%83%e3%82%af%e3%83%a9%e3%82%a4%e3%83%88%e3%81%a7%e8%9b%8d%e5%85%89%e3%82%92%e7%99%ba%e3%81%99%e3%82%8b%e8%9b%8d%e5%85%89%e3%82%bf%e3%83%b3%e3%83%91%e3%82%af%e8%b3%aa%e3%83%a2","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3277","title":{"rendered":"\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\u3067\u86cd\u5149\u3092\u767a\u3059\u308b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u30e2\u30c7\u30eb\u30923D\u30d7\u30ea\u30f3\u30bf\u30fc\u3067\u4f5c\u3063\u3066\u307f\u305f"},"content":{"rendered":"<p>\u4ee5\u524d\u30013D\u30d7\u30ea\u30f3\u30bf\u30fc\u3092\u4f7f\u3063\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7acb\u4f53\u69cb\u9020\u30e2\u30c7\u30eb\u3092\u30d7\u30ea\u30f3\u30c8\u3059\u308b<a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3008\" target=\"_blank\" rel=\"noopener\">\u30a8\u30f3\u30c8\u30ea\u30fc<\/a>\u3092\u66f8\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u305d\u306e\u5f8c\u3082\u7740\u5b9f\u306b\uff08\uff1f\uff09\u8272\u3005\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6253\u3061\u51fa\u3057\u3066\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u9175\u6bcd\u306e\u89e3\u7cd6\u7cfb\u9175\u7d20\u30bf\u30f3\u30d1\u30af\u8cea<\/p>\n<figure id=\"attachment_3278\" aria-describedby=\"caption-attachment-3278\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?attachment_id=3278\" rel=\"attachment wp-att-3278\"><img fetchpriority=\"high\" decoding=\"async\" class=\"size-large wp-image-3278\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_2868-1024x477.jpg\" alt=\"\" width=\"580\" height=\"270\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_2868-1024x477.jpg 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_2868-300x140.jpg 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_2868-768x358.jpg 768w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3278\" class=\"wp-caption-text\">Yeast Glycolytic Proteins<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>GFP\u3068GFP\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea<\/p>\n<figure id=\"attachment_3280\" aria-describedby=\"caption-attachment-3280\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?attachment_id=3280\" rel=\"attachment wp-att-3280\"><img decoding=\"async\" class=\"size-large wp-image-3280\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3632-1024x689.jpg\" alt=\"\" width=\"580\" height=\"390\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3632-1024x689.jpg 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3632-300x202.jpg 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3632-768x517.jpg 768w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3280\" class=\"wp-caption-text\">GFP &amp; GFP Binding Protein<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u7dd1\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08EGFP\uff09\u3068\u4e73\u6e05\u30bf\u30f3\u30d1\u30af\u8cea\uff08\u30a2\u30eb\u30d6\u30df\u30f3\u3068\u30b0\u30ed\u30d6\u30ea\u30f3\uff09\u2015\u30d0\u30ea\u4ed8\u304d<\/p>\n<figure id=\"attachment_3279\" aria-describedby=\"caption-attachment-3279\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?attachment_id=3279\" rel=\"attachment wp-att-3279\"><img decoding=\"async\" class=\"size-large wp-image-3279\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3290-934x1024.jpg\" alt=\"\" width=\"580\" height=\"636\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3290-934x1024.jpg 934w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3290-274x300.jpg 274w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3290-768x842.jpg 768w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3279\" class=\"wp-caption-text\">GFP &amp; Whey proteins<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u305d\u306e\u4ed6\u3082\u308d\u3082\u308d\u3002\u30c7\u30b9\u30af\u6a2a\u306e\u68da\u306e\u4e0a\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30e2\u30c7\u30eb\u304c\u30b4\u30ed\u30b4\u30ed\u3068\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u4eca\u307e\u3067\u6253\u3061\u51fa\u3057\u305f\u4e2d\u3067\u4e00\u756a\u304b\u3063\u3053\u3088\u304b\u3063\u305f\u306e\u306f\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u53d7\u5bb9\u4f53\u3067\u3059\u3002<\/p>\n<figure id=\"attachment_3281\" aria-describedby=\"caption-attachment-3281\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?attachment_id=3281\" rel=\"attachment wp-att-3281\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-3281\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3493-1024x683.jpg\" alt=\"\" width=\"580\" height=\"387\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3493-1024x683.jpg 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3493-300x200.jpg 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3493-768x512.jpg 768w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3281\" class=\"wp-caption-text\">Glutamate Receptor<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u6700\u8fd1\u3001\u4ee5\u524d\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u3092\u3054\u89a7\u4e0b\u3055\u3063\u305f\u696d\u8005\u306b\u96fb\u8a71\u3092\u3044\u305f\u3060\u304d\u3001\u300c\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\uff08\u7d2b\u5916\u7dda\uff09\u3067\u767a\u5149\u3059\u308b\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u304c\u3042\u308b\u306e\u3060\u3051\u308c\u3069\u4f7f\u3063\u3066\u307f\u307e\u305b\u3093\u304b\u300d\u3068\u8a00\u308f\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u8272\u306f\u3001\u7dd1\u30fb\u9752\u30fb\u8d64\u30fb\u9ec4\u8272\u304c\u3042\u308b\u3068\u306e\u3053\u3068\u3002\u300c\u3053\u308c\u3067\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u30d7\u30ea\u30f3\u30c8\u3057\u305f\u3089\u307e\u3055\u306b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3058\u3083\u3093\uff01\u300d\u3068\u601d\u3063\u305f\u79c1\u306f\u65e9\u901f\u30b5\u30f3\u30d7\u30eb\u3092\u9001\u3063\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u7dd1\u30fb\u9752\u30fb\u9ec4\u8272GFP\u30fbBFP\u30fbYFP\u306b\u5bfe\u5fdc\u3059\u308b\u3060\u308d\u3046\u3068\u3044\u3046\u3053\u3068\u3067GFP\u306e3D\u69cb\u9020\u3067\u4f5c\u308a\u307e\u3057\u305f\u3002\u8d64\u306fRFP\u306e\u4e2d\u3067\u3082\u3088\u304f\u4f7f\u308f\u308c\u308bmCherry\u306e3D\u69cb\u9020\u3067\u4f5c\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u6253\u3061\u51fa\u3057\u305f\u3089\u3053\u3093\u306a\u611f\u3058\u3002\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\u3092\u5f53\u3066\u3066\u3044\u306a\u3044\u3068\u304d\u3067\u3082GFP\u306f\u82e5\u5e72\u7dd1\u3063\u307d\u3044\u3067\u3059\u3002mCherry\u306fGFP\u3068\u5c11\u3057\u5f62\u304c\u9055\u3046\u3093\u3067\u3059\u306d\u3002<\/p>\n<figure id=\"attachment_3283\" aria-describedby=\"caption-attachment-3283\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?attachment_id=3283\" rel=\"attachment wp-att-3283\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-3283\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3693-1024x498.jpg\" alt=\"\" width=\"580\" height=\"282\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3693-1024x498.jpg 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3693-300x146.jpg 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3693-768x373.jpg 768w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3283\" class=\"wp-caption-text\">\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\u3092\u5f53\u3066\u3066\u3044\u306a\u3044BFP, GFP, YFP, mCherry<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u3055\u3066\u3001\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\u3092\u3042\u3066\u3066\u307f\u307e\u3059\u3002\u96fb\u6c17\u6cf3\u52d5\u3067\u5206\u96e2\u3057\u305fDNA\u3092\u898b\u308b\u305f\u3081\u306b\u4f7f\u3046\u88c5\u7f6e\u3067\u4e0b\u304b\u3089\u7167\u3089\u3057\u3066\u307f\u307e\u3059\u3002<\/p>\n<figure id=\"attachment_3284\" aria-describedby=\"caption-attachment-3284\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?attachment_id=3284\" rel=\"attachment wp-att-3284\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-3284\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3704-1024x502.jpg\" alt=\"\" width=\"580\" height=\"284\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3704-1024x502.jpg 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3704-300x147.jpg 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3704-768x377.jpg 768w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2016\/12\/IMG_3704.jpg 1802w\" sizes=\"(max-width: 580px) 100vw, 580px\" \/><\/a><figcaption id=\"caption-attachment-3284\" class=\"wp-caption-text\">\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\u7167\u5c04\u3067\u5149\u308bBFP, GFP, YFP, mCherry<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u3053\u308a\u3083\u7dba\u9e97\u3084\u308f\uff01<\/p>\n<p>\u3044\u3064\u3082\u306f\u79c1\u304c\u6b21\u3005\u3068\u6253\u3061\u51fa\u3059\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30e2\u30c7\u30eb\u306b\u4e00\u5207\u8208\u5473\u3092\u793a\u3055\u306a\u3044\u5b66\u751f\u3069\u3082\u304c\u308f\u3089\u308f\u3089\u3068\u96c6\u307e\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u5149\u308b\u3063\u3066\u5927\u4e8b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u4f7f\u3063\u305f\u306e\u306f<a href=\"http:\/\/www.central-techno.co.jp\/\" target=\"_blank\" rel=\"noopener\">\u30bb\u30f3\u30c8\u30e9\u30eb\u30c6\u30af\u30ce\u682a\u5f0f\u4f1a\u793e<\/a>\u306e\u30eb\u30df\u30b7\u30b9\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3067\u3059\u3002\u4e00\u822c\u7684\u306aABS\u6a39\u8102\u3068\u540c\u3058\u6761\u4ef6\u3067\u30d7\u30ea\u30f3\u30c8\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4ee5\u524d\u30013D\u30d7\u30ea\u30f3\u30bf\u30fc\u3092\u4f7f\u3063\u3066\u30bf&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3284,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[138],"tags":[],"class_list":["post-3277","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-3d"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3277","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3277"}],"version-history":[{"count":8,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3277\/revisions"}],"predecessor-version":[{"id":3424,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3277\/revisions\/3424"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3284"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3277"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3277"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3277"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}