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class=\"title\">\u79c1\u304c\u5148\u8f29\u304b\u3089\u805e\u3044\u305f\u795e\u8a71\u306b\u306f\u3001\u300c\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30fc\u30ec\u30fc\u30b7\u30e7\u30f3\u306f\u3001\u9ad8\u96fb\u5727\u3092\u304b\u3051\u3066\u7d30\u80de\u306b\u7a74\u3092\u958b\u3051\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u7d30\u80de\u5185\u306b\u5165\u308c\u308b\u3002\u7d30\u80de\u306f\u30dc\u30ed\u30dc\u30ed\u306b\u306a\u308b\u306e\u3067\u3001<strong>\u5f8c\u57f9\u990a\u306f\u7d76\u5bfe\u306b\u5fc5\u8981\u3060<\/strong>\u300d\u3068\u3044\u3046\u306e\u3082\u3042\u308a\u307e\u3057\u305f\u3002\u7814\u7a76\u5ba4\u3067\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30fc\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u591a\u7528\u3059\u308b\u3088\u3046\u306b\u306a\u3063\u3066\u304b\u3089\u6570\u5e74\u3001\u305a\u3063\u3068\u3053\u308c\u3092\u4fe1\u3058\u3066\u3084\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u65b0\u3057\u304f\u6765\u305f\u30e9\u30dc\u30e1\u30f3\u30d0\u30fc\u306b\u306f\u305d\u3046\u4f1d\u3048\u3066\u5b9f\u9a13\u3092\u3084\u3063\u3066\u3082\u3089\u3063\u3066\u3044\u307e\u3057\u305f\u3002<\/p>\n<p>\u3068\u3053\u308d\u304c\u3042\u308b\u65e5\u3001\u30e9\u30dc\u3067\u306e\u30ad\u30e3\u30ea\u30a2\u306e\u9577\u3044\u535a\u58eb\u8ab2\u7a0b\u306e\u5b66\u751f\u306b\u805e\u3044\u3066\u307f\u308b\u3068\u3001\u300c<strong>\u3048\uff1f\uff01 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href=\"http:\/\/www.funakoshi.co.jp\/contents\/469\">\u300c\u30d2\u30fc\u30c8\u30b7\u30e7\u30c3\u30af\u4e0d\u8981\u306a\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u300d\u3068\u3044\u3046\u3082\u306e\u304c\u58f2\u308a\u51fa\u3055\u308c\u3066\u3044\u307e\u3059<\/a>\u3002\u3044\u3084\u305d\u3082\u305d\u3082\u30d2\u30fc\u30c8\u30b7\u30e7\u30c3\u30af\u5fc5\u8981\u306a\u3044\u3093\u3067\u3059\u3051\u3069\u30fb\u30fb\u30fb\u3002<\/p>\n<p>\u88dc\u8db3\uff13\uff1a\u6628\u65e5\u3084\u3063\u3066\u307f\u305f\u5b9f\u9a13\u306e\u7d50\u679c\u3092\u3064\u3051\u3066\u304a\u304d\u307e\u3059\u3002pUC18\u3067TOP10\u682a\u3092\u5f62\u8cea\u8ee2\u63db\u3001LBamp\u30d7\u30ec\u30fc\u30c8\u306b\u6492\u3044\u3066\u3044\u307e\u3059\u3002\u5de6\u304c\uff11\u5206\u30d7\u30ed\u30c8\u30b3\u30eb\u3001\u53f3\u304c\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\uff08\uff11\uff0e\uff15\uff48\uff52\uff09\u3067\u3059\u3002<\/p>\n<figure id=\"attachment_3603\" aria-describedby=\"caption-attachment-3603\" style=\"width: 628px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/E_coli_plate.jpg\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-3603 size-large\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/E_coli_plate-1024x501.jpg\" alt=\"\" width=\"628\" height=\"307\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/E_coli_plate-1024x501.jpg 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/E_coli_plate-300x147.jpg 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/E_coli_plate-768x375.jpg 768w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/E_coli_plate-956x467.jpg 956w\" sizes=\"(max-width: 628px) 100vw, 628px\" \/><\/a><figcaption id=\"caption-attachment-3603\" class=\"wp-caption-text\">\u5f62\u8cea\u8ee2\u63db\u4f53\u306e\u30b3\u30ed\u30cb\u30fc\u3002\u5de6\u304c\uff11\u5206\u3001\u53f3\u304c\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\u3002\u30b3\u30ed\u30cb\u30fc\u304c\u898b\u3084\u3059\u304f\u306a\u308b\u3088\u3046\u753b\u50cf\u88dc\u6b63\u3057\u3066\u3042\u308a\u307e\u3059\u3002<\/figcaption><\/figure>\n<p>\u88dc\u8db3\uff14\uff1a<a href=\"https:\/\/lifescience.toyobo.co.jp\/detail\/detail.php?product_detail_id=147\" target=\"_blank\" rel=\"noopener noreferrer\">\u6771\u6d0b\u7d21\u306e\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\uff08Competent Quick DH5\u03b1\uff09<\/a>\u3067\u306f\u3001\u6c37\u4e0a\uff15\u5206\u3001\u30d2\u30fc\u30c8\u30b7\u30e7\u30c3\u30af\uff14\uff12\u2103\u30fb\uff13\uff10\u79d2\u3067\u30d7\u30ec\u30fc\u30c8\u306b\u6492\u304f\u3068\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u6297\u751f\u7269\u8cea\u306e\u9055\u3044\u3068SOC\u306b\u3088\u308b\u5f8c\u57f9\u990a\u306e\u52b9\u679c\u304c\u8abf\u3079\u3089\u308c\u3066\u3044\u307e\u3059\u3002Kan\u3067\u306f\u57f9\u5730\u306e\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u306e\u6fc3\u5ea6\u304c\u9ad8\u3059\u304e\u3066\u306f\u3060\u3081\u3001Tet\u3084Cm\u306a\u3069\u3092\u7528\u3044\u308b\u969b\u306b\u306f\u5f8c\u57f9\u990a\u304c\u5fc5\u9808\u306e\u3088\u3046\u3067\u3059\u3002\u2192 \u5f53\u521d\u30ea\u30f3\u30af\u306ePDF\u306f\u306a\u304f\u306a\u308a<a href=\"https:\/\/lifescience.toyobo.co.jp\/faq\/category_list.php?faq_category_id=63\">FAQ<\/a><a href=\"https:\/\/lifescience.toyobo.co.jp\/faq\/category_list.php?faq_category_id=63\">\u306e\u30da\u30fc\u30b8<\/a>\u304c\u3067\u304d\u3066\u3044\u308b\u3088\u3046\u3067\u3059\u3002<\/p>\n<p><strong>\u3059\u3079\u3066\u306e\u6297\u751f\u7269\u8cea\u306b\u5bfe\u5fdc\u3067\u304d\u308b\u306e\u304c\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u826f\u3055\u3068\u3044\u3046\u3053\u3068\u306e\u3088\u3046\u3067\u3059\u3002<\/strong><\/p>\n<p>20190518 \u8ffd\u8a18<\/p>\n<p>\u300c\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30fc\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u969b\u3001\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3084\u30ad\u30e5\u30d9\u30c3\u30c8\u306f\u6c37\u4e0a\u3067\u51b7\u3084\u3057\u3066\u304a\u304f\u3053\u3068\u300d\u3002\u3053\u308c\u3082\u5148\u8f29\u304b\u3089\u3059\u308a\u8fbc\u307e\u308c\u308b<strong>\u5fc5\u8981\u4e0d\u53ef\u6b20\u306a\u30ce\u30a6\u30cf\u30a6<\/strong>\u3067\u3059\u3002\u3068\u3053\u308d\u304c\u3001\u3053\u308c\u304c\u3044\u3089\u306a\u3044\u3069\u3053\u308d\u304b\u3080\u3057\u308d\u5ba4\u6e29\u3067\u3084\u3063\u305f\u65b9\u304c\u52b9\u7387\u304c\u826f\u3044\u3068\u3044\u3046\u8ad6\u6587\u304c\u3067\u307e\u3057\u305f\uff08\u6587\u732e4\u3002E\u541b\u60c5\u5831\u3042\u308a\u304c\u3068\u3046\u3054\u3056\u3044\u307e\u3059\uff09\u3002<\/p>\n<p>\u6700\u8fd1\u3001\u79c1\u305f\u3061\u306e\u7814\u7a76\u5ba4\u3067\u306f\u9762\u5012\u304f\u3055\u3044\u306e\u3067\u6c37\u3067\u51b7\u3084\u3059\u306e\u3092\u3084\u3081\u3066\u3044\u305f\u306e\u3067\u3059\u304c\u3001\u306a\u3093\u3068\u305d\u306e\u65b9\u304c\u52b9\u7387\u304c\u826f\u304b\u3063\u305f\u3068\u306f\u30fb\u30fb\u30fb\u3002\u3046\u308c\u3057\u3044\u3088\u3046\u306a\u60b2\u3057\u3044\u3088\u3046\u306a\u3002<\/p>\n<hr \/>\n<p>\u6587\u732e<\/p>\n<ol>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16876281\">Role of membrane potential on artificial transformation of E. coli with plasmid DNA.\u00a0<\/a>Panja S, Saha S, Jana B, Basu T.\u00a0<span class=\"jrnl\" title=\"Journal of biotechnology\">J Biotechnol<\/span>. 2006 Dec 15;127(1):14-20. Epub 2006 Jun 23.\u00a0PMID:16876281<\/li>\n<li style=\"text-align: left;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/8602370\">High efficiency 5 min transformation of Escherichia coli.\u00a0<\/a>Pope B, Kent HM.\u00a0<span class=\"jrnl\" title=\"Nucleic acids research\">Nucleic Acids Res<\/span>. 1996 Feb 1;24(3):536-7. No abstract available.\u00a0PMID: 8602370<\/li>\n<li style=\"text-align: left;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28261629\">Impact of heat shock step on bacterial transformation efficiency.\u00a0<\/a>Rahimzadeh M, Sadeghizadeh M, Najafi F, Arab S, Mobasheri H.\u00a0<span class=\"jrnl\" title=\"Molecular biology research communications\">Mol Biol Res Commun<\/span>. 2016 Dec;5(4):257-261.\u00a0PMID:28261629<\/li>\n<li>\n<p class=\"title\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27095488\">Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency. <\/a><span style=\"font-size: inherit;\">Tu Q, Yin J, Fu J, Herrmann J, Li Y, Yin Y, Stewart AF, M\u00fcller R, Zhang Y. <\/span><span class=\"jrnl\" style=\"font-size: inherit;\" title=\"Scientific reports\">Sci Rep<\/span><span style=\"font-size: inherit;\">. 2016 Apr 20;6:24648. doi: 10.1038\/srep24648. PMID:27095488<\/span><\/p>\n<div class=\"aux\">\n<div class=\"resc\"><\/div>\n<\/div>\n<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u30a8\u30f3\u30c8\u30ea\u30fc\u540d\u3001\u5c11\u3057\u5909\u3048\u307e\u3057\u305f\uff08&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3592,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[19,25],"tags":[],"class_list":["post-3572","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-19","category-25"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3572","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3572"}],"version-history":[{"count":34,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3572\/revisions"}],"predecessor-version":[{"id":4319,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3572\/revisions\/4319"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3592"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3572"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3572"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3572"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}