{"id":3621,"date":"2017-11-15T09:22:37","date_gmt":"2017-11-15T00:22:37","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3621"},"modified":"2017-11-15T13:02:08","modified_gmt":"2017-11-15T04:02:08","slug":"%e5%a4%a7%e8%85%b8%e8%8f%8c%e3%81%a7%e6%80%9d%e3%81%84%e3%81%a3%e3%81%8d%e3%82%8agfp%e3%82%92%e7%99%ba%e7%8f%be%e3%81%97%e3%81%a6%e3%81%bf%e3%81%9f","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3621","title":{"rendered":"\u5927\u8178\u83cc\u3067\u601d\u3044\u3063\u304d\u308aGFP\u3092\u767a\u73fe\u3057\u3066\u307f\u305f"},"content":{"rendered":"<p>\u4ee5\u524d\u3001<a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=373\">\u9175\u6bcd\u3067\u6b7b\u306c\u307b\u3069GFP\u3092\u767a\u73fe\u3057\u3066\u307f\u305f\u3089\u9175\u6bcd\u306e\u30b3\u30ed\u30cb\u30fc\u304c\u5e7b\u60f3\u7684\u306a\u7dd1\u8272\u306b\u306a\u308b<\/a>\u3053\u3068\u3092\u3053\u306e\u30d6\u30ed\u30b0\u3067\u66f8\u304d\u307e\u3057\u305f\u3002\u4eca\u56de\u3001\u305f\u307e\u305f\u307e\u5927\u8178\u83cc\u3067\u601d\u3044\u3063\u304d\u308aGFP\u3092\u767a\u73fe\u3059\u308b\u6a5f\u4f1a\u304c\u3042\u308a\u307e\u3057\u305f\u306e\u3067\u3001\u305d\u306e\u89b3\u5bdf\u7d50\u679c\u3092\u3002\u6761\u4ef6\u306f\u3001pGLO in TOP10\u682a\u3001LBamp + 40mM arabinose\u306e\u6761\u4ef6\u3067\u3059\u3002<\/p>\n<figure id=\"attachment_3624\" aria-describedby=\"caption-attachment-3624\" style=\"width: 628px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/ecca51316ba983d9f09e0a53b0967832.png\"><img fetchpriority=\"high\" decoding=\"async\" class=\"size-large wp-image-3624\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/ecca51316ba983d9f09e0a53b0967832-1024x463.png\" alt=\"\" width=\"628\" height=\"284\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/ecca51316ba983d9f09e0a53b0967832-1024x463.png 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/ecca51316ba983d9f09e0a53b0967832-300x136.png 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/ecca51316ba983d9f09e0a53b0967832-768x347.png 768w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/ecca51316ba983d9f09e0a53b0967832-956x432.png 956w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2017\/11\/ecca51316ba983d9f09e0a53b0967832.png 1567w\" sizes=\"(max-width: 628px) 100vw, 628px\" \/><\/a><figcaption id=\"caption-attachment-3624\" class=\"wp-caption-text\">GFP\u3092\u5927\u91cf\u767a\u73fe\u3057\u3066\u3044\u308b\u5927\u8178\u83cc\u306e\u30b3\u30ed\u30cb\u30fc\u3002\u5de6\uff1a\u305d\u306e\u307e\u307e\u898b\u305f\u3082\u306e\u3001\u53f3\u304c\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\u3092\u3042\u3066\u305f\u3082\u306e\u3002<\/figcaption><\/figure>\n<p>\u30a2\u30e9\u30d3\u30ce\u30fc\u30b9\u306b\u3088\u308aGFP\u3092\u8a98\u5c0e\u3057\u3066\u3044\u307e\u3059\u3002\u30a2\u30e9\u30d3\u30ce\u30fc\u30b9\u306a\u3057\u306e\u30b3\u30ed\u30cb\u30fc\u3068\u6bd4\u3079\u308b\u3068\u5897\u6b96\u304c\u9045\u304f\u306a\u3063\u3066\u3044\u308b\u306e\u3067\u3001\u300c\u6b7b\u306c\u307b\u3069\u300d\u3068\u306f\u3044\u3044\u307e\u305b\u3093\u304c\u3001\u5897\u6b96\u963b\u5bb3\u304c\u8d77\u304d\u308b\u7a0b\u5ea6\u306b\u306fGFP\u304c\u5927\u91cf\u767a\u73fe\u3057\u3066\u3044\u307e\u3059\u3002\u30d6\u30e9\u30c3\u30af\u30e9\u30a4\u30c8\u3092\u3042\u3066\u308b\u3068\u304d\u308c\u3044\u306b\u5149\u308a\u307e\u3059\uff08\u53f3\uff09\u304c\u3001\u9175\u6bcd\u306e\u6642\u306e\u3088\u3046\u306b\u3001\u8089\u773c\u3067\u898b\u3048\u308b\u9ec4\u7dd1\u8272\u306e\u304d\u308c\u3044\u306a\u30b3\u30ed\u30cb\u30fc\u306f\u4f5c\u308a\u307e\u305b\u3093\uff08\u5de6\uff1a\u82e5\u5e72\u8584\u7dd1\u306b\u898b\u3048\u308b\u7a0b\u5ea6\u3067\u3059\uff09\u3002\u79c1\u306f\u3053\u308c\u3092\u898b\u3066\u9006\u306b\u3001\u300c\u9175\u6bcd\u3059\u3052\u3048\u3058\u3083\u3093\uff01\u300d\u3068\u601d\u3063\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u3002<\/p>\n<p>\u305f\u3060\u3001\u3053\u308c\u306fGFPuv\u3068\u3044\u3046\u30d0\u30ea\u30a2\u30f3\u30c8\u3092\u4f7f\u3063\u3066\u3044\u308b\u306e\u3067\u3001\u305d\u306e\u305b\u3044\u3067\u53ef\u8996\u5149\u306e\u3082\u3068\u3067\u306f\u3042\u307e\u308a\u7dd1\u306b\u898b\u3048\u306a\u3044\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u3082\u3063\u3068\u300c\u7dd1\u300d\u306a\u5927\u8178\u83cc\u306e\u30b3\u30ed\u30cb\u30fc\u3092\u77e5\u3063\u3066\u3044\u308b\u65b9\u306f\u9023\u7d61\u9802\u3051\u308b\u3068\u3046\u308c\u3057\u3044\u3067\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4ee5\u524d\u3001\u9175\u6bcd\u3067\u6b7b\u306c\u307b\u3069GFP\u3092\u767a&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3624,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[25,133,137],"tags":[],"class_list":["post-3621","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-25","category-133","category-137"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3621","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3621"}],"version-history":[{"count":4,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3621\/revisions"}],"predecessor-version":[{"id":3628,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3621\/revisions\/3628"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3624"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3621"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3621"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3621"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}