{"id":3630,"date":"2017-11-28T14:39:08","date_gmt":"2017-11-28T05:39:08","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3630"},"modified":"2018-02-15T15:57:13","modified_gmt":"2018-02-15T06:57:13","slug":"%e9%85%b5%e6%af%8d%e3%81%ae%e9%81%ba%e4%bc%9d%e5%ad%90%e3%83%a9%e3%82%a4%e3%83%96%e3%83%a9%e3%83%aa%e3%83%bc%e3%81%82%e3%82%8c%e3%81%93%e3%82%8c-1","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3630","title":{"rendered":"\u9175\u6bcd\u306e\u907a\u4f1d\u5b50\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3042\u308c\u3053\u308c (1)"},"content":{"rendered":"<p>\u3053\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\uff08\u30b7\u30ea\u30fc\u30ba\uff09\u3067\u306f\u3001\u51fa\u82bd\u9175\u6bcd\uff08S. cerevisiae\uff09\u306e\u907a\u4f1d\u5b50\u3092\u5bfe\u8c61\u3068\u3057\u3066\u4f5c\u3089\u308c\u3066\u304d\u305f\uff08\u305d\u3057\u3066\u624b\u306b\u5165\u308b\uff09\u907a\u4f1d\u5b50\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306b\u3064\u3044\u3066\u307e\u3068\u3081\u3066\u3044\u304d\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u9577\u304f\u306a\u3063\u305f\u306e\u3067\u30a8\u30f3\u30c8\u30ea\u30fc\u3092\u5206\u3051\u3066\u66f8\u3044\u3066\u3044\u304d\u307e\u3059\uff08\u6700\u5f8c\u306b\u7d71\u5408\u3059\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\uff09\u3002<\/p>\n<hr 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S.cerevisiae<\/em>\uff09\u306b\u9650\u5b9a\u3059\u308b\u3068\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306f\u307b\u307c\u3059\u3079\u3066\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u4f5c\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u306f\u3001<em>S. cerevisiae<\/em>\u306b\u975e\u5e38\u306b\u4f7f\u3044\u52dd\u624b\u306e\u826f\u3044\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u3042\u308b\u304b\u3089\u3067\u3059\u3002\u305d\u3057\u3066\u3001\u53e4\u304f\u306f\u30e9\u30f3\u30c0\u30e0\u306b\u633f\u5165\u3055\u308c\u305f\u30b2\u30ce\u30e0DNA\u3084cDNA\u304c\u7528\u3044\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u30dd\u30b9\u30c8\u30b2\u30ce\u30e0\u306e\u6642\u4ee3\u304b\u3089\u306f\u540c\u5b9a\u3057\u305f\u907a\u4f1d\u5b50\u9818\u57df\u3092\u7d44\u307f\u8fbc\u3093\u3060\u300c\u4e2d\u8eab\u304c\u5206\u304b\u3063\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u300d\u304c\u4f5c\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u3066\u304d\u3066\u3044\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u3001\u7279\u306b\u305d\u308c\u3089\u306e\u300c\u4e2d\u8eab\u304c\u308f\u304b\u3063\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u300d\u306b\u3069\u306e\u3088\u3046\u306a\u3082\u306e\u304c\u3042\u308b\u304b\u3001\u305d\u306e\u7279\u5fb4\u306a\u3069\u3092\u307e\u3068\u3081\u3066\u307f\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<h5>1. Yeat Genomic Tiling Collection\uff08\u540c\u5b9a\u3055\u308c\u305f\u30a4\u30f3\u30b5\u30fc\u30c8\u3092\u6301\u3064\u30b2\u30ce\u30e0\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\uff09<\/h5>\n<p>\u53e4\u5178\u7684\u306b\u300c\u30b2\u30ce\u30e0\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u300d\u3068\u8a00\u3046\u306e\u306f\u3001\u901a\u5e38\u30b2\u30ce\u30e0\u3092\u7269\u7406\u7684\u3042\u308b\u3044\u306f\u751f\u5316\u5b66\u7684\u306b\u3042\u308b\u7a0b\u5ea6\u306e\u5927\u304d\u3055\u306b\u5206\u65ad\u3057\u3001\u305d\u308c\u3092\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u30e9\u30f3\u30c0\u30e0\u306b\u7d44\u307f\u8fbc\u3093\u3067\u4f5c\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3053\u306e\u30e9\u30f3\u30c0\u30e0\u306a\u30a4\u30f3\u30b5\u30fc\u30c8\u3092\u6301\u3064\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306f\u901a\u5e38\u3001\u5168\u3066\u6df7\u305c\u3089\u308c\u305f\u30d7\u30fc\u30eb\u3068\u3057\u3066\u7dad\u6301\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30d7\u30fc\u30eb\u3092\u4e00\u5ea6\u306b\u9175\u6bcd\u306b\u5f62\u8cea\u8ee2\u63db\u3057\u3066\u3084\u308b\u3053\u3068\u3067\u3001\u5f62\u8cea\u8ee2\u63db\u4f53\u306e\u305d\u308c\u305e\u308c\u306e\u30b3\u30ed\u30cb\u30fc\u306f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u4e2d\u304b\u3089\u7570\u306a\u3063\u305f\uff11\u3064\u306e\u30af\u30ed\u30fc\u30f3\u3092\u6301\u3064\u3053\u3068\u306b\u306a\u308a\u3001\u305d\u308c\u305e\u308c\u306e\u30b3\u30ed\u30cb\u30fc\u3092\u76ee\u7684\u306b\u5fdc\u3058\u3066\u30a2\u30c3\u30bb\u30a4\u3059\u308b\u3053\u3068\u3067\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u3092\u5358\u96e2\u3059\u308b\u3002\u3053\u308c\u304c\u3001\u30b2\u30ce\u30e0\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u7528\u3044\u305f\u5b9f\u9a13\u306e\u901a\u5e38\u306e\u6d41\u308c\u3067\u3059\u3002<\/p>\n<p>\u907a\u4f1d\u5b50\u9818\u57df\u3092\u542b\u3080\u3088\u3046\u306b\u9069\u5ea6\u306a\u9577\u3055\u3092\u6301\u3063\u305f\u30a4\u30f3\u30b5\u30fc\u30c8\u304c\u3001\u5168\u30b2\u30ce\u30e0\u9818\u57df\u3092\u30ab\u30d0\u30fc\u3059\u308b\u3088\u3046\u306b\u542b\u307e\u308c\u3066\u3044\u308b\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306f\u3001\u300c\u826f\u3044\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u300d\u3068\u3044\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30e9\u30f3\u30c0\u30e0\u306a\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u306f\u3001\u3069\u308c\u304f\u3089\u3044\u306e\u30af\u30ed\u30fc\u30f3\u3092\u8abf\u3079\u305f\u3089\u30b2\u30ce\u30e0\u5168\u57df\u3092\u8abf\u3079\u305f\u3053\u3068\u306b\u306a\u308b\u306e\u304b\u3001\u7406\u8ad6\u7684\u306b\u306f\u7b97\u51fa\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3082\u3068\u3082\u3068\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u826f\u3057\u60aa\u3057\u306b\u3088\u3063\u3066\u306f\u3001\u3044\u304f\u3089\u63a2\u7d22\u3057\u3066\u3082\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u304c\u5f97\u3089\u308c\u306a\u304b\u3063\u305f\u308a\u3001\u3061\u3083\u3093\u3068\u30b2\u30ce\u30e0\u5168\u57df\u3092\u30ab\u30d0\u30fc\u3067\u304d\u306a\u304b\u3063\u305f\u308a\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u3053\u3046\u3084\u3063\u3066\u30e9\u30f3\u30c0\u30e0\u306b\u4f5c\u3063\u305f\u30b2\u30ce\u30e0\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u914d\u5217\u3092\u4e00\u3064\u305a\u3064\u6c7a\u5b9a\u3057\u3001\u305d\u308c\u305e\u308c\u304c\u30b2\u30ce\u30e0\u9818\u57df\u306e\u3069\u3053\u306b\u3042\u308b\u304b\u3092\u6c7a\u5b9a\u3057\u3001\u305d\u306e\u60c5\u5831\u3092\u3082\u3068\u306b\u5404\u30af\u30ed\u30fc\u30f3\u3092\u3042\u308b\u7a0b\u5ea6\u306e\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u3092\u6301\u305f\u305b\u3066\u30b2\u30ce\u30e0\u306e\u5168\u9818\u57df\u3092\u30ab\u30d0\u30fc\u3059\u308b\u3088\u3046\u306b\u4e26\u3079\u305f\uff08tiling\u3057\u305f\uff09\u306e\u304c\u3001\u3053\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u3059\u3002\u306f\u3058\u3081\u306f\u30de\u30eb\u30c1\u30b3\u30d4\u30fc\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u304c\u4f5c\u3089\u308c\u307e\u3057\u305f\uff08\u6587\u732e\uff11 \u904e\u5270\u767a\u73fe\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306b\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff09\u3002\u3055\u3089\u306b\u540c\u3058\u30c1\u30fc\u30e0\u304b\u3089\u3001\u30b7\u30f3\u30b0\u30eb\u30b3\u30d4\u30fc\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3082\u4f5c\u3089\u308c\u307e\u3057\u305f\uff08\u6587\u732e\uff12\uff09\u3002<\/p>\n<p>\u3053\u308c\u3089\u306f\u30b2\u30ce\u30e0\u4e0a\u306e98.5%\u3092\u30ab\u30d0\u30fc\u3059\u308b1,750\u7a0b\u5ea6\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u304b\u3089\u306a\u308b\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u3001\u3053\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u4f7f\u3048\u3070\u6bd4\u8f03\u7684\u5c11\u306a\u3044\u6570\u3092\u63a2\u7d22\u3059\u308b\u3053\u3068\u3067\u30b2\u30ce\u30e0\u4e0a\u306e\u3059\u3079\u3066\u306e\u907a\u4f1d\u5b50\u3092\u8abf\u3079\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u306f\u3001\u633f\u5165\u3055\u308c\u305f\u907a\u4f1d\u5b50\uff08\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u306f\u3001\u30b2\u30ce\u30e0\u4e0a\u3068\u540c\u3058\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304b\u3089\u767a\u73fe\u3055\u308c\u3001\u6a19\u8b58\u30fb\u7cbe\u88fd\u7528\u306e\u30bf\u30b0\u306f\u3064\u3044\u3066\u3044\u307e\u305b\u3093\u3002\u591a\u30b3\u30d4\u30fc\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306f\u3001<a href=\"http:\/\/dharmacon.gelifesciences.com\/cdnas-and-orfs\/non-mammalian-cdnas-and-orfs\/yeast\/yeast-genomic-tiling-collection\/?rdr=true&amp;LangType=1041&amp;id=17179927359\" target=\"_blank\" rel=\"noopener\">Yeast Genomic Tiling Collection\u3068\u3057\u3066Dharmacon\u304b\u3089\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059<\/a>\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>2018.2.15 \u8ffd\u8a18<\/p>\n<p>\u3053\u306e\u3088\u3046\u306aTiling Collection\u4f5c\u6210\u306e\u8a66\u307f\u306f\u3001\u5b9f\u306f\u30b2\u30ce\u30e0\u30b7\u30fc\u30b1\u30f3\u30b9\u304c\u5b8c\u4e86\u3059\u308b\u524d\u304b\u3089\u306a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u306f\u3001\u30e9\u30f3\u30c0\u30e0\u306b\u7d44\u307f\u8fbc\u3093\u3060\u30b2\u30ce\u30e0DNA\u65ad\u7247\u306e\u5236\u9650\u9175\u7d20\u306e\u5207\u65ad\u30d1\u30bf\u30fc\u30f3\u3092\u5229\u7528\u3057\u3066\u9806\u756a\u3092\u306a\u3089\u3079\u308b\u3068\u3044\u3046\u65b9\u6cd5\u304c\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u30af\u30ed\u30fc\u30f3\uff08\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\uff09\u306f\u3001Ordered Clone\u3068\u547c\u3070\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u5b9f\u306f\u51fa\u82bd\u9175\u6bcd\u306eOrdered Clone\u306e\u4e00\u90e8\u306f\u3001\u79c1\u304c\u5927\u5b66\u9662\u6642\u4ee3\u306b\u6240\u5c5e\u3057\u3066\u3044\u305f\u7814\u7a76\u5ba4\u3067\u4f5c\u3089\u308c\u3066\u3044\u305f\u306e\u3067\u3059\uff08\u4f8b\u3068\u3057\u3066\u6587\u732e\uff13\uff09\u3002<\/p>\n<p>\u5f53\u6642\u3001\u03bb\u30d5\u30a1\u30fc\u30b8\u3092\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u4f5c\u6210\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u03bb\u30d5\u30a1\u30fc\u30b8\u30d9\u30af\u30bf\u30fc\u306b\u306f\u5927\u304d\u306aDNA\u65ad\u7247\u304c\u633f\u5165\u3067\u304d\u308b\u3068\u3044\u3046\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308b\u3082\u306e\u306e\u3001\u9175\u6bcd\u3067\u306e\u6a5f\u80fd\u89e3\u6790\u306b\u4f7f\u304a\u3046\u3068\u3059\u308b\u3068\u4e00\u624b\u9593\u5fc5\u8981\u306b\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u3053\u308c\u304c\u3053\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u304c\u73fe\u5728\u307e\u3067\u6b8b\u3089\u306a\u304b\u3063\u305f\u7406\u7531\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u6587\u732e<\/p>\n<ol>\n<li class=\"title\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18246075\">A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae.\u00a0<\/a>Jones GM, Stalker J, Humphray S, West A, Cox T, Rogers J, Dunham I, Prelich G.\u00a0<span class=\"jrnl\" title=\"Nature methods\">Nat Methods<\/span>. 2008 Mar;5(3):239-41. doi: 10.1038\/nmeth.1181. Epub 2008 Feb 3.\u00a0PMID:18246075<\/li>\n<li class=\"title\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20641023\">A systematic CEN library of the Saccharomyces cerevisiae genome.\u00a0<\/a>Hvorecny KL, Prelich G.\u00a0<span class=\"jrnl\" title=\"Yeast (Chichester, England)\">Yeast<\/span>. 2010 Oct;27(10):861-5. doi: 10.1002\/yea.1783.\u00a0PMID:20641023<\/li>\n<li>\n<p class=\"title\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/1522073\">An ordered clone bank for chromosome I of Saccharomyces cerevisiae.\u00a0<\/a>Tanaka S, Yoshikawa A, Isono K.\u00a0<span class=\"jrnl\" title=\"Journal of bacteriology\">J Bacteriol<\/span>. 1992 Sep;174(18):5985-7.\u00a0PMID:1522073<\/p>\n<\/li>\n<\/ol>\n<p>\u305d\u306e\uff12\u306b\u7d9a\u304f<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3053\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\uff08\u30b7\u30ea\u30fc\u30ba\uff09\u3067\u306f&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3639,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[25,139,8,137,9],"tags":[],"class_list":["post-3630","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-25","category-139","category-8","category-137","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3630","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3630"}],"version-history":[{"count":10,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3630\/revisions"}],"predecessor-version":[{"id":3669,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3630\/revisions\/3669"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3639"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3630"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3630"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3630"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}