{"id":3645,"date":"2018-01-04T12:34:12","date_gmt":"2018-01-04T03:34:12","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3645"},"modified":"2018-01-04T14:37:49","modified_gmt":"2018-01-04T05:37:49","slug":"cas9%e3%81%af%e8%a9%a6%e9%a8%93%e7%ae%a1%e5%86%85%e3%81%a7%e3%82%82dna%e3%82%92%e3%82%81%e3%81%a3%e3%81%a1%e3%82%83%e5%88%87%e3%82%8b","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3645","title":{"rendered":"Cas9\u306f\u8a66\u9a13\u7ba1\u5185\u3067\u3082DNA\u3092(\u3081\u3063\u3061\u3083)\u5207\u308b!?"},"content":{"rendered":"<p>CRISPR-Cas9\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3001\u8fd1\u3044\u3046\u3061\u306b\u9593\u9055\u3044\u306a\u304f\u30ce\u30fc\u30d9\u30eb\u8cde\u3092\u3068\u308b\u9769\u65b0\u7684\u6280\u8853\u3067\u3059\u3002\u6211\u304c\u9175\u6bcd\u306b\u304a\u3044\u3066\u3082\u3001\u3053\u306e\u6280\u8853\u3092\u4f7f\u3063\u3066\u76db\u3093\u306b\u30b2\u30ce\u30e0\u306e\u5927\u898f\u6a21\u306a\u7de8\u96c6\u304c\u884c\u308f\u308c\u3001\u6bce\u65e5\u306e\u3088\u3046\u306b\u8ad6\u6587\u304c\u51fa\u3066\u3044\u307e\u3059\u3002\u79c1\u3082\u4e00\u5fdc\u3053\u306e\u5b9f\u9a13\u7cfb\u3092\u52d5\u304b\u3057\u3066\u307f\u3066\u30e1\u30ea\u30c3\u30c8\u3092\u5b9f\u611f\u3057\u307e\u3057\u305f\u3002\u30fb\u30fb\u30fb\u3067\u3059\u304c\u305d\u308c\u3060\u3051\u3002\u3082\u3068\u3082\u3068\u76f8\u540c\u7d44\u63db\u3048\u6d3b\u6027\u304c\u5f37\u304f\u3001\u3042\u308b\u7a0b\u5ea6\u601d\u3044\u901a\u308a\u306e\u30b2\u30ce\u30e0\u64cd\u4f5c\u304c\u3067\u304d\u308b\u9175\u6bcd\u3067\u306f\u3001\u4eca\u306e\u3068\u3053\u308dCRISPR-Cas9\u3092\u4f7f\u3046\u5fc5\u7136\u6027\u306f\u611f\u3058\u3066\u3044\u307e\u305b\u3093\u3002\u3053\u306e\u6280\u8853\u65e5\u9032\u6708\u6b69\u306a\u306e\u3067\u3001\u6210\u719f\u3057\u3066\u300c\u67af\u308c\u305f\u6280\u8853\u300d\u306b\u306a\u308b\u307e\u3067\u3057\u3070\u3089\u304f\u69d8\u5b50\u3092\u898b\u3088\u3046\u3068\u601d\u3063\u3066\u3044\u307e\u3057\u305f\u3002<\/p>\n<p>\u3068\u3053\u308d\u304c\u3001\u3042\u308b\u3068\u304d\u7814\u7a76\u8005\u4ef2\u9593\u306eNY\u3055\u3093\u304b\u3089\u300c<strong>Cas9\u3001in vitro\uff08\u8a66\u9a13\u7ba1\u5185\uff09\u3067\u3082DNA\u3092\u3081\u3063\u3061\u3083\u5207\u308a\u307e\u3059\u3088\uff01<\/strong>\u300d\u3068\u6559\u3048\u3066\u3044\u305f\u3060\u304d\u307e\u3057\u305f\u3002\u300c\u3081\u3063\u3061\u3083\u5207\u308b\u300d\u3068\u3044\u3046\u8868\u73fe\u306f\u3042\u3093\u307e\u308a\u79d1\u5b66\u7684\u3067\u306f\u306a\u3044\u3067\u3059\u304c\u3001\u306a\u3093\u304b\u3059\u3054\u304f\u4f7f\u3048\u308b\u9175\u7d20\u611f\u6e80\u8f09\u3067\u3059\u3002<\/p>\n<p>\u8a66\u9a13\u7ba1\u5185\u3067Cas\uff19\u3092\u4f7f\u3063\u3066\u4f55\u304c\u3067\u304d\u308b\uff08\u4f55\u304c\u3057\u305f\u3044\uff09\u306e\u304b\uff1f\u3000<strong>DNA\u4e0a\u306e\u72d9\u3063\u305f\u90e8\u4f4d\u3092\u5207\u65ad\u3067\u304d\u307e\u3059\u3002<\/strong>\u79c1\u305f\u3061\u306e\u7528\u9014\u3067\u306f\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u7279\u5b9a\u306e\u90e8\u4f4d\u3092\u5207\u65ad\u3057\u305f\u3044\u3002\u79c1\u305f\u3061\u306f <a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/~hisaom\/HMwiki\/index.php?Gap-Repair%20cloning%E3%82%92%E4%BD%BF%E3%81%8A%E3%81%86%21\" target=\"_blank\" rel=\"noopener\">Gap-Repair Cloning<\/a> \u3068\u3044\u3046\u65b9\u6cd5\u3067\u3001\u9175\u6bcd\u306e\u76f8\u540c\u7d44\u63db\u3048\u6d3b\u6027\u3092\u5229\u7528\u3057\u3066\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u69cb\u7bc9\u3059\u308b\u306e\u3067\u3059\u304c\u3001\u3053\u306e\u6642\u3001\u7e4b\u3052\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u76f4\u9396\u72b6\u306b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u5207\u308a\u305f\u3044\u3068\u3053\u308d\u306b\u5236\u9650\u9175\u7d20\u306e\u5207\u65ad\u90e8\u4f4d\u304c\u3042\u308c\u3070\u305d\u308c\u3092\u4f7f\u3048\u3070\u826f\u3044\u306e\u3067\u3059\u304c\u3001\u3044\u3064\u3082\u3046\u307e\u3044\u3053\u3068\u305d\u3053\u306b\u3042\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u6b21\u306e\u624b\u6bb5\uff08\u305d\u3057\u3066\u3088\u304f\u4f7f\u3046\u624b\u6bb5\uff09\u3068\u3057\u3066\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u5168\u4f53\u3092PCR\u3067\u5897\u5e45\u3057\u76f4\u9396\u72b6\u306b\u3057\u307e\u3059\u3002\u3053\u3046\u3059\u308c\u3070\u3001\u307b\u307c\u81ea\u7531\u306b\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u69cb\u7bc9\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u306e\u3067\u3059\u304c\u3001\u9577\u3044DNA\u304cPCR\u3067\u5897\u3048\u306b\u304f\u304b\u3063\u305f\u308a\u3001PCR\u306b\u3088\u308b\u30a8\u30e9\u30fc\u304c\u751f\u3058\u305f\u308a\u3059\u308b\u61f8\u5ff5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u305d\u3053\u3067Cas9\u3067\u3059\u3002\u5207\u308a\u305f\u3044\u90e8\u4f4d\u3092\u6307\u5b9a\u3059\u308b\u30ac\u30a4\u30c9RNA\uff08gRNA\uff09\u3092\u4f5c\u3063\u3066Cas9\u3068\u6df7\u305c\u3066\u3084\u308c\u3070\u3001<strong>\u30ab\u30b9\u30bf\u30e0\u5236\u9650\u9175\u7d20\u306e\u51fa\u6765\u4e0a\u304c\u308a<\/strong>\u3067\u3059\u3002\u78ba\u304b\u306b\u8a00\u308f\u308c\u3066\u307f\u308c\u3070\u305d\u3046\u306a\u306e\u3067\u3059\u304c\u3001\u7cbe\u88fd\u3057\u305f\u9175\u7d20\u304c\u8a66\u9a13\u7ba1\u5185\u3067\u30d0\u30ea\u30d0\u30ea\u50cd\u304f\uff08\u3081\u3063\u3061\u3083\u5207\u308b\uff09\u304b\u3069\u3046\u304b\u306f\u3001\u9175\u7d20\u306e\u6d3b\u6027\u3084\u5b89\u5b9a\u6027\u3082\u95a2\u4fc2\u3059\u308b\u306e\u3067\u3001\u305d\u3046\u7c21\u5358\u306a\u8a71\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p>\u524d\u7f6e\u304d\u304c\u9577\u304f\u306a\u308a\u307e\u3057\u305f\u304c\u3001\u305d\u3046\u3044\u3046\u308f\u3051\u3067\u3001Cas9\u3092\u4f7f\u3063\u3066\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5207\u3063\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u306f\u305d\u306e\u30ec\u30dd\u30fc\u30c8\u3067\u3059\u3002\u7d50\u679c\u3068\u3057\u3066\u306f\u3001<strong>\u5272\u3068\u9069\u5f53\u306b\u3084\u308a\u307e\u3057\u305f\u304cCas9\u306f\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u3061\u3083\u3093\u3068\u5207\u3063\u3066\u304f\u308c\u307e\u3057\u305f<\/strong>\u3002<\/p>\n<hr \/>\n<p>\u3055\u3066\u3001\u307e\u305a\u306f\u6750\u6599\u3067\u3059\u3002Cas9\u3068gRNA\u306e\u4f5c\u6210\u30ad\u30c3\u30c8\u306f\u3001\u304b\u306eNEB\u304b\u3089\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u3002<a href=\"https:\/\/www.nebj.jp\/products\/detail\/1923\" target=\"_blank\" rel=\"noopener\">Cas9\u306f\u5c0f\u30ed\u30c3\u30c8\u306720\u56de\u52069,500\u5186<\/a>\u3002<a href=\"https:\/\/www.nebj.jp\/products\/detail\/1923\" target=\"_blank\" rel=\"noopener\">gRNA\u4f5c\u6210\u30ad\u30c3\u30c820\u56de\u520672,000\u5186\u3002<\/a>\u3053\u308c\u306b\u52a0\u3048\u3066\u3001gRNA\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u306a\u308b55-mer\u306e\u30ab\u30b9\u30bf\u30e0\u30aa\u30ea\u30b4DNA\u3092\u5408\u6210\u3057\u307e\u3059\u3002<a href=\"http:\/\/nebiocalculator.neb.com\/#!\/sgrna\" target=\"_blank\" rel=\"noopener\">gRNA\u306e\u30c7\u30b6\u30a4\u30f3\u306fNEB\u306e\u30a6\u30a7\u30d6\u30b5\u30a4\u30c8\u3067\u7c21\u5358\u306b\u3067\u304d\u308b<\/a>\u3088\u3046\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u4eca\u56de\u306f\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5225\u3005\u306e\u5834\u6240\u3067\u5207\u65ad\u3059\u308b2\u3064\u306egRNA\u7528\u30aa\u30ea\u30b4\u3092\u4f5c\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u30ad\u30c3\u30c8\u5316\u3055\u308c\u3066\u3044\u3066\u3001\u3084\u308b\u3053\u3068\u306f\u305d\u3046\u8907\u96d1\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002(\uff11) gRNA\u4f5c\u6210\u30ad\u30c3\u30c8\u306b\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u3092\u6df7\u305c\u3066\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3057gRNA\u3092\u4f5c\u6210\u3001(\uff12) \uff11\u306egRNA\u3092Cas9\u3068\u6df7\u305c\u3066\u3057\u3070\u3089\u304f\u304a\u3044\u3066\u3001\u5207\u65ad\u3057\u305f\u3044\u30d7\u30e9\u30b9\u30df\u30c9\u3068\u6df7\u305c\u3066\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3002\u5b9f\u969b\u306b\u5fc5\u8981\u306a\u306e\u306f\u3053\u308c\u3060\u3051\u3067\u3059\u3002<\/p>\n<p>RNA\u3092\u6271\u3046\u306e\u3067RNase\u5bfe\u7b56\u304c\u5fc5\u8981\u3067\u3059\uff08\u6c17\u3092\u3064\u304b\u3046\u306e\u3067\u5acc\u3044\u306a\u5b9f\u9a13\u3067\u3059\uff09\u3002\u3061\u3083\u3093\u3068\u3084\u308b\u306a\u3089\uff11\u306e\u5f8c\u306b\u3001gRNA\u304c\u3061\u3083\u3093\u3068\u5408\u6210\u3067\u304d\u3066\u3044\u308b\u304b\u96fb\u6c17\u6cf3\u52d5\u3057\u3066\u78ba\u8a8d\u3001gRNA\u306e\u7cbe\u88fd\u3001gRNA\u306e\u5b9a\u91cf\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306eDNase\u306b\u3088\u308b\u6d88\u5316\u3001DNase\u306e\u4e0d\u6d3b\u5316\u3001\u304c\u5fc5\u8981\u3002\uff12\u306e\u5f8c\u306b\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306b\u3088\u308bCas9\u306e\u6d88\u5316\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u308c\u3089\u306b\u5fc5\u8981\u306a\u8a66\u85ac\u306f\u30ad\u30c3\u30c8\u306b\u306f\u5165\u3063\u3066\u3044\u307e\u305b\u3093\u3002\u4eca\u56de\u306f\u624b\u629c\u304d\u3057\u3066\u3053\u308c\u3089\u3092\u3059\u3079\u3066\u3059\u3063\u98db\u3070\u3057\u307e\u3057\u305f\u3002gRNA\u306f\u30ad\u30c3\u30c8\u306b\u66f8\u304b\u308c\u3066\u3044\u308b\u304a\u304a\u3088\u305d\u306e\u4f5c\u6210\u91cf\u3092\u53c2\u8003\u306b\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u304a\u5f85\u3061\u304b\u306d\u306e\u7d50\u679c\u3067\u3059\u3002\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u3067\u96fb\u6c17\u6cf3\u52d5\u3057\u3066\u30c1\u30a7\u30c3\u30af\u3057\u307e\u3057\u305f\u3002\u5de6\u304b\u3089\u3001Cas9\u306b2\u3064\u306egRNA\uff082144, 2145\uff09\u3092\u6df7\u305c\u305f\u3082\u306e\u30012144\u3060\u3051\u30012145\u3060\u3051\u3092\u6df7\u305c\u305f\u3082\u306e\u3002<\/p>\n<figure id=\"attachment_3646\" aria-describedby=\"caption-attachment-3646\" style=\"width: 102px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2018\/01\/Cas9_digest1.jpg\"><img decoding=\"async\" class=\"size-full wp-image-3646\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2018\/01\/Cas9_digest1.jpg\" alt=\"\" width=\"102\" height=\"271\" \/><\/a><figcaption id=\"caption-attachment-3646\" class=\"wp-caption-text\">Cas9\u306b\u3088\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u5207\u65ad\uff11<\/figcaption><\/figure>\n<p>\u3053\u306e\u5199\u771f\u3001\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc\u7b49\u304c\u306a\u304f\u3066\u6050\u7e2e\u3067\u3059\u304c\u3001\u4e0b\u306e\u65b9\u306b\u30e2\u30ef\u30c3\u3068\u898b\u3048\u308b\u306e\u306f\u5206\u5b50\u91cf\u304c\u5c0f\u3055\u3044\u306e\u3067\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u3060\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u3067\u3001\u809d\u5fc3\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3059\u304c\u3001\u306a\u3093\u304b\u306f\u3063\u304d\u308a\u3057\u306a\u3044\u3002\u3061\u3083\u3093\u3068\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u3042\u308b\u306f\u305a\u306a\u306e\u306b\u8584\u3044\u3002\u5206\u5b50\u91cf\u304c\u5927\u304d\u3044\u3068\u3053\u308d\u3067\u30b9\u30e1\u30a2\u30fc\u306b\u306a\u3063\u3066\u3044\u308b\u3088\u3046\u306b\u3082\u898b\u3048\u308b\u3002<\/p>\n<p>Cas9\u3084gRNA\u4f5c\u6210\u30ad\u30c3\u30c8\u306b\u5165\u3063\u3066\u3044\u308b\u9175\u7d20\u304c\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u304f\u3063\u3064\u3044\u3066\u3046\u307e\u304f\u6cf3\u52d5\u3067\u304d\u3066\u3044\u306a\u3044\u306e\u304b\u3082\u3057\u308c\u306a\u3044\u3002\u305d\u3053\u3067\u3001\u71b1\u51e6\u7406\uff0865\u210310\u5206\uff09\u3092\u3057\u3066\u305d\u308c\u3089\u306e\u9175\u7d20\u3092\u5931\u6d3b\u3055\u305b\u3066\u307f\u308b\u3053\u3068\u306b\u3057\u307e\u3057\u305f\u3002<\/p>\n<figure id=\"attachment_3647\" aria-describedby=\"caption-attachment-3647\" style=\"width: 123px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2018\/01\/Cas9-2.jpg\"><img decoding=\"async\" class=\"size-full wp-image-3647\" src=\"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2018\/01\/Cas9-2.jpg\" alt=\"\" width=\"123\" height=\"194\" \/><\/a><figcaption id=\"caption-attachment-3647\" class=\"wp-caption-text\">Cas9\u306b\u3088\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u5207\u65ad\uff12<\/figcaption><\/figure>\n<p>\u304a\u304a\u3001\u3061\u3083\u3093\u3068\u898b\u3048\u305f\uff01\u5207\u308c\u3066\u308b\uff01 \uff12\u30ab\u6240\u5207\u65ad\u306e\u7d50\u679c\u304b\u3089\u307f\u3066\u3001\u4e21\u65b9\u306e\u90e8\u4f4d\u3067\u3061\u3083\u3093\u3068\u5207\u308c\u3066\u3044\u308b\u3088\u3046\u3067\u3059\u3002\u300c\u72d9\u3063\u305f\u90e8\u4f4d\u3067DNA\u3092\u5207\u308b\u300d\u3068\u3044\u3046\u306e\u304c\u3053\u3093\u306a\u306b\u7c21\u5358\u306b\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u3093\u3067\u3059\u306d\u3002\u30b9\u30b4\u3044\uff01<\/p>\n<p>\u3053\u308c\u304c\u4f7f\u3048\u308b\u7cfb\u3068\u3057\u3066\u30eb\u30fc\u30c1\u30f3\u306b\u306a\u308b\u304b\u3069\u3046\u304b\u306f\u3001\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u6700\u9069\u5316\u3092\u3082\u3046\u3059\u3053\u3057\u3084\u3063\u3066\u3001\u3069\u308c\u304f\u3089\u3044\u306e\u624b\u9593\u3067\u3069\u308c\u304f\u3089\u3044\u306e\u30b3\u30b9\u30c8\u3067\u5b9f\u884c\u53ef\u80fd\u304b\u3001\u3069\u308c\u304f\u3089\u3044\u5b89\u5b9a\u304b\u3092\u8abf\u3079\u3066\u307f\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002PCR\u306b\u52dd\u3066\u308b\u304b\u3069\u3046\u304b\u304c\uff11\u3064\u306e\u6307\u6a19\u3067\u3059\u306d\u3002<\/p>\n<p>\u6700\u5f8c\u306b\u3001Cas9\u306e\u60c5\u5831\u3092\u4e0b\u3055\u3063\u305fNY\u3055\u3093\u3001\u5b9f\u9a13\u3092\u624b\u4f1d\u3063\u3066\u304f\u308c\u305fHaku\u3055\u3093\u306b\u611f\u8b1d\u3057\u307e\u3059\u3002<\/p>\n<hr \/>\n<p>\u3044\u304f\u3064\u304b\u88dc\u8db3\u3067\u3059\u3002<\/p>\n<ul>\n<li>\u3053\u306e\u5b9f\u9a13\u3067\u4e00\u756a\u30b3\u30b9\u30c8\u304c\u304b\u304b\u308b\u306e\u306fgRNA\u5408\u6210\u3067\u3001\u5358\u7d14\u8a08\u7b97\u3067\u306f\u4e00\u56de3,600\u5186\u3002\u305f\u3060\u3001gRNA\u306f\u4e00\u5ea6\u5408\u6210\u3059\u308b\u3068100\u56de\u5206\u304f\u3089\u3044\u306f\u4f7f\u3048\u308b\u3002\u4eca\u56de\u306f\u6fc3\u5ea6\u8a08\u7b97\u3092\u8aa4\u3063\u3066100\u500d\u4f4d\u5165\u308c\u3066\u3057\u307e\u3063\u305f\u3002<\/li>\n<li>\u5207\u65ad\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u6eb6\u6db2\u306b\u306fRNaseA\u304c\u5165\u3063\u3066\u3044\u308b\u3002\u305d\u308c\u3067\u3082\u30d7\u30e9\u30b9\u30df\u30c9\u5207\u65ad\u304c\u3067\u304d\u305f\u3002Cas9-gRNA\u304c\u4e00\u65e6\u3067\u304d\u308b\u3068gRNA\u306fRNaseA\u306b\u3088\u308b\u6d88\u5316\u304b\u3089\u5b88\u3089\u308c\u308b\u306e\u304b\u3082\u3057\u308c\u306a\u3044\u3002gRNA\u3092\u9593\u9055\u3063\u3066\u5927\u91cf\u306b\u5165\u308c\u305f\u304b\u3089\u5927\u4e08\u592b\u3060\u3063\u305f\u306e\u304b\u3082\u3057\u308c\u306a\u3044\u3002<\/li>\n<li>\u9175\u7d20\u91cf\u3092\u3069\u308c\u3060\u3051\u6e1b\u3089\u305b\u308b\u304b\u3001\u3069\u308c\u304f\u3089\u3044\u306e\u91cf\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f55\u5206\u3067\u6d88\u5316\u3059\u308b\u304b\u304c\u691c\u8a0e\u8ab2\u984c\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>CRISPR-Cas9\u306b\u3088\u308b\u30b2&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3661,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[25,133],"tags":[],"class_list":["post-3645","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-25","category-133"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3645","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3645"}],"version-history":[{"count":13,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3645\/revisions"}],"predecessor-version":[{"id":3660,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3645\/revisions\/3660"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3661"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3645"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3645"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3645"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}