{"id":3784,"date":"2018-09-21T03:08:35","date_gmt":"2018-09-20T18:08:35","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3784"},"modified":"2018-09-22T00:49:38","modified_gmt":"2018-09-21T15:49:38","slug":"%e9%85%b5%e6%af%8d%e3%81%ae%e9%81%ba%e4%bc%9d%e5%ad%90%e3%83%a9%e3%82%a4%e3%83%96%e3%83%a9%e3%83%aa%e3%83%bc%e3%81%82%e3%82%8c%e3%81%93%e3%82%8c-2","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3784","title":{"rendered":"\u9175\u6bcd\u306e\u907a\u4f1d\u5b50\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3042\u308c\u3053\u308c (2)"},"content":{"rendered":"

\u305a\u3044\u3076\u3093\u9593\u304c\u7a7a\u3044\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u304c\u3001\u300c\u9175\u6bcd\u306e\u907a\u4f1d\u5b50\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3042\u308c\u3053\u308c\u300d\u306e\u305d\u306e\uff12\u3067\u3059\u3002\u5b66\u4f1a\u3067\u300c\u7d9a\u304d\u3092\u66f8\u3044\u3066\u307b\u3057\u3044\u300d\u3068\u3044\u3046\u30ea\u30af\u30a8\u30b9\u30c8\u3092\u3082\u3089\u3044\u307e\u3057\u305f\u306e\u3067\u9811\u5f35\u3063\u3066\u66f8\u304d\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u305d\u306e\uff11\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u306f\u3053\u3061\u3089\u3067\u3059<\/a>\u3002<\/p>\n

\u524d\u56de\u306f\u3001\u30e9\u30f3\u30c0\u30e0\u306b\u4f5c\u3063\u305f\u30b2\u30ce\u30e0\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u914d\u5217\u3092\u4e00\u3064\u305a\u3064\u6c7a\u5b9a\u3057\u3001\u305d\u308c\u305e\u308c\u304c\u30b2\u30ce\u30e0\u9818\u57df\u306e\u3069\u3053\u306b\u3042\u308b\u304b\u3092\u6c7a\u5b9a\u3057\u3001\u305d\u306e\u60c5\u5831\u3092\u3082\u3068\u306b\u5404\u30af\u30ed\u30fc\u30f3\u3092\u3042\u308b\u7a0b\u5ea6\u306e\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u3092\u6301\u305f\u305b\u3066\u30b2\u30ce\u30e0\u306e\u5168\u9818\u57df\u3092\u30ab\u30d0\u30fc\u3059\u308b\u3088\u3046\u306b\u4e26\u3079\u305f\u3001\u300cTiling collection\u300d\u306b\u3064\u3044\u3066\u89e3\u8aac\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u3053\u3053\u304b\u3089\u306f\u3001Tilling collection\u306e\u3088\u3046\u306b\u30e9\u30f3\u30c0\u30e0\u306a\u914d\u5217\u3092\u7d44\u307f\u8fbc\u3093\u3067\u5f8c\u304b\u3089\u914d\u5217\u3092\u6c7a\u3081\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u4f5c\u308b\u524d\u304b\u3089\u7d44\u307f\u8fbc\u3080\u30a4\u30f3\u30b5\u30fc\u30c8\u304c\u540c\u5b9a\u3055\u308c\u3066\u3044\u308b\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306b\u3064\u3044\u3066\u89e3\u8aac\u3057\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u306f\u5171\u901a\u3057\u3066PCR\u3067\u5897\u5e45\u3057\u305f\u30a4\u30f3\u30b5\u30fc\u30c8\u3092\u7d44\u307f\u8fbc\u3093\u3067\u3044\u307e\u3059\u3002PCR\u3067\u306f\u5897\u5e45\u3057\u305f\u3044DNA\u65ad\u7247\u306b\u7279\u7570\u7684\u306a\u914d\u5217\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3057\u307e\u3059\u304b\u3089\u3001\u3053\u306e\u6642\u70b9\u3067\u3069\u3046\u3044\u3046\u914d\u5217\u3092\u7d44\u307f\u8fbc\u3080\u304b\u304c\u3042\u3089\u304b\u3058\u3081\u308f\u304b\u3063\u3066\u3044\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u826f\u3044\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u4f5c\u308b\u305f\u3081\u306b\u306f\u3001\u540c\u5b9a\u3055\u308c\u3066\u3044\u308b\u907a\u4f1d\u5b50\u3092\u3067\u304d\u308b\u3060\u3051\u7db2\u7f85\u3059\u308b\u3088\u3046\u306bPCR\u3067\u5897\u3084\u3057\u3066\u30d9\u30af\u30bf\u30fc\u306b\u7d44\u307f\u8fbc\u3080\u5fc5\u8981\u304c\u3042\u308a\u3001\u307e\u305f\u7d44\u307f\u8fbc\u307f\u305f\u3044\u30a4\u30f3\u30b5\u30fc\u30c8\u304c\u3061\u3083\u3093\u3068\u7d44\u307f\u8fbc\u307e\u308c\u3066\u3044\u308b\u304b\uff08\u5909\u7570\u3082\u5165\u3063\u3066\u3044\u306a\u3044\u304b\uff09\u3092\u78ba\u8a8d\u3059\u308b\u4f5c\u696d\u3082\u5fc5\u8981\u3067\u3059\u304b\u3089\u3001\u4f5c\u6210\u306b\u306f\u76f8\u5f53\u306e\u52b4\u529b\uff08\u3068\u304a\u91d1\uff09\u304c\u304b\u304b\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u4f7f\u7528\u76ee\u7684\u306b\u5fdc\u3058\u3066\u7d44\u307f\u8fbc\u307e\u308c\u308b\u30a4\u30f3\u30b5\u30fc\u30c8\u3084\u30d9\u30af\u30bf\u30fc\u304c\u5909\u308f\u308b\u306e\u3067\u3001\u8907\u6570\u7a2e\u985e\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u304c\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u3067\u306f\u3001\u305d\u3046\u3084\u3063\u3066\u4f5c\u3089\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u4e2d\u3067\u4ee3\u8868\u7684\u306a\u3082\u306e\u306b\u3064\u3044\u3066\u89e3\u8aac\u3057\u305f\u3044\u3068\u601d\u3044\u307e\u3059\uff08\u898b\u51fa\u3057\u306e\u756a\u53f7\u306fTiling collection\u304b\u3089\u306e\u7d9a\u304d\u306b\u306a\u3063\u3066\u3044\u307e\u3059\uff09\u3002<\/p>\n

\u3056\u3063\u304f\u308a\u3068\u5206\u985e\u3059\u308b\u3068\u3001\u6a19\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\uff08\u305d\u306e\u307e\u307e\uff09\u904e\u5270\u767a\u73fe\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u305f\u3082\u306e\u3068\u3001\u6a19\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7cbe\u88fd\u3057\u3066\u751f\u5316\u5b66\u89e3\u6790\u306b\u6301\u3061\u8fbc\u3080\u305f\u3081\u306e\u7cbe\u88fd\u30bf\u30b0\u3092\u3064\u3051\u305f\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u3002\u904e\u5270\u767a\u73fe\u3057\u305f\u3068\u304d\u306b\u8d77\u304d\u308b\u7d30\u80de\u306e\u5909\u5316\u3092\u6307\u6a19\u306b\u7279\u5b9a\u306e\u6a5f\u80fd\u3092\u3082\u3064\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u63a2\u308b\u306e\u304c\uff11\u306e\u4e3b\u306a\u7528\u9014\u3067\u3001\u7279\u5b9a\u306e\u751f\u5316\u5b66\u7684\u6d3b\u6027\u3092\u6301\u3064\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u63a2\u308b\u306e\u304c\uff12\u306e\u4e3b\u306a\u7528\u9014\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u3055\u3089\u306b\u3001\u6a19\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30cd\u30a4\u30c6\u30a3\u30d6\u306a\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304b\u3089\u767a\u73fe\u3055\u305b\u308b\u3082\u306e\u3068\u3001\u8a98\u5c0e\u53ef\u80fd\u306a\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u5236\u5fa1\u4e0b\u304b\u3089\u767a\u73fe\u3055\u305b\u308b\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u3002\u51fa\u82bd\u9175\u6bcd\u306e\u5834\u5408\u3001\u8a98\u5c0e\u53ef\u80fd\u306a\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3057\u3066\u306f\u3001\u30b0\u30eb\u30b3\u30fc\u30b9\u57f9\u5730\u3067\u306f\u6291\u5236\u3055\u308c\u3066\u3044\u3066\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u57f9\u5730\u3067\u8a98\u5c0e\u304c\u304b\u304b\u308bGAL1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u307b\u3068\u3093\u3069\u306e\u30b1\u30fc\u30b9\u3067\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

2. gTOW6000 collection (native promoter, \u30bf\u30b0\u7121\u3057, URA3, leu2d\uff09<\/h5>\n

5751\u7a2e\u985e\u306e\u907a\u4f1d\u5b50\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u30b3\u30fc\u30c9\u9818\u57df\uff08ORF\uff09\uff0b\u30cd\u30a4\u30c6\u30a3\u30d6\u306a\u5236\u5fa1\u9818\u57df\uff09\u304c\u30012\u00b5m plasmid\u3068\u3044\u3046\u591a\u30b3\u30d4\u30fc\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u7d44\u307f\u8fbc\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306bleu2\u3068\u3044\u3046\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50\u304c\u3042\u308b\u305f\u3081\u3001\u30ed\u30a4\u30b7\u30f3\u306e\u306a\u3044\u57f9\u5730\u3067\u3053\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306f100\u30b3\u30d4\u30fc\u4ee5\u4e0a\u306b\u306a\u308a\u307e\u3059\u3002\u30cd\u30a4\u30c6\u30a3\u30d6\u306a\u5236\u5fa1\u9818\u57df\u3092\u6301\u3063\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u30b3\u30d4\u30fc\u6570\u5206\u3060\u3051\u30cd\u30a4\u30c6\u30a3\u30d6\u306a\u767a\u73fe\u91cf\u304b\u3089\u306e\u904e\u5270\u767a\u73fe\u3092\u8d77\u3053\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u524d\u30a8\u30f3\u30c8\u30ea\u30fc\u306eTiling collection\u306b\u8fd1\u3044\u7279\u5fb4\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u304c\u3001\u907a\u4f1d\u5b50\u304c\u500b\u5225\u306b\u30af\u30ed\u30fc\u30f3\u3055\u308c\u3066\u3044\u308b\u3068\u3053\u308d\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u30d6\u30ed\u30b0\u306e\u7b46\u8005\u3089\u304c\u4f5c\u3063\u305f\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u3067\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u5168\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306fNBRP-yeast\u304b\u3089\u5165\u624b\u53ef\u80fd\u3067\u3059<\/a>\u3002<\/p>\n

3. GST collection\uff08GAL1 promoter, N\u672b\u7aefGST\u30bf\u30b0, 2\u00b5m plasmid, URA3, leu2d\uff09<\/h5>\n

5280\u7a2e\u985e\u306eORF\u304c\u3001glutathione-S-transfrase (GST) \u3068\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u3001GAL1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304b\u3089\u767a\u73fe\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u30b3\u30f3\u30b9\u30c8\u30e9\u30af\u30c8\u304c\u591a\u30b3\u30d4\u30fc\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u30af\u30ed\u30fc\u30f3\u3055\u308c\u3066\u304a\u308a\u3001\u3055\u3089\u306bleu2d\u3092\u3082\u3064\u306e\u3067\u30ed\u30a4\u30b7\u30f3\u306e\u306a\u3044\u57f9\u5730\u3067\u975e\u5e38\u306b\u9ad8\u30ec\u30d9\u30eb\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3092\u8d77\u3053\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306fDharmacon\u304b\u3089\u8cfc\u5165\u3067\u304d\u307e\u3059\uff08\u65e5\u672c\u306e\u4ee3\u7406\u5e97\u306f\u30d5\u30ca\u30b3\u30b7\uff09<\/a>\u3002<\/p>\n

4. Movable ORF collection (GAL1 promoter, C\u672b\u7aefHis6-HA-ProteinA\u30bf\u30b0,\u00a02\u00b5m plasmid, URA3, Gateway\uff09<\/h5>\n

5854\u7a2e\u985e\u306eORF\u304c\u3001His6-HA-Protein A\u3068\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u3001GAL1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304b\u3089\u767a\u73fe\u3055\u308c\u307e\u3059\u3002\u591a\u30b3\u30d4\u30fc\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u30af\u30ed\u30fc\u30f3\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306e\u7279\u5fb4\u306f\u3001Gateway\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u3046\u3053\u3068\u3067\u5404ORF\u3092\u5225\u306e\u30d9\u30af\u30bf\u30fc\u306b\u4e00\u62ec\u3057\u3066\u79fb\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u3068\u3053\u308d\u306b\u3042\u308a\u307e\u3059\uff08\u305d\u308c\u3067movable\u3068\u547c\u3070\u308c\u3066\u3044\u308b\uff09\u3002\u3053\u308c\u3082Dharmacon\u304b\u3089\u767a\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u65e5\u672c\u306e\u4ee3\u7406\u5e97\u306f\u30d5\u30ca\u30b3\u30b7\uff09<\/a>\u3002<\/p>\n

\u4ee5\u4e0a\u3001\u9175\u6bcd\uff08Saccharomyces cerevisiae\uff09\u306e\u4ee3\u8868\u7684\u306a\u30d7\u30e9\u30b9\u30df\u30c9\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306e\u4f5c\u6210\u306b\u7528\u3044\u3089\u308c\u3066\u3044\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u69cb\u6210\u306a\u3069\u306b\u3064\u3044\u3066\u306f\u3001\u79c1\u306e\uff08\u6614\u306e\uff1f\uff09\u30a6\u30a7\u30d6\u30da\u30fc\u30b8\u306b\u89e3\u8aac\u3057\u3066\u3042\u308a\u307e\u3059\uff08\u51fa\u82bd\u9175\u6bcd\u306egTOW\u7528\u30d7\u30e9\u30b9\u30df\u30c9<\/a>)\u3002\u307e\u305f\u3001\u4e0a\u8a18gTOW6000\u30b3\u30ec\u30af\u30b7\u30e7\u30f3\u306e\u958b\u767a\u79d8\u8a71\uff08\uff1f\uff09\u3082\u540c\u30da\u30fc\u30b8\u306b\u9577\u6587\u3067\u89e3\u8aac\u3057\u3066\u3044\u307e\u3059\uff08gTOW6000\u8ad6\u6587<\/a>\uff09\u3002\u8208\u5473\u304c\u3042\u308b\u65b9\u306f\u8aad\u3093\u3067\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u305d\u306e\uff13\u306b\u7d9a\u304f\uff08\u304b\u3082\u3057\u308c\u306a\u3044\uff09\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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