{"id":3790,"date":"2018-09-21T04:19:00","date_gmt":"2018-09-20T19:19:00","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3790"},"modified":"2018-09-21T04:22:17","modified_gmt":"2018-09-20T19:22:17","slug":"%ef%bc%88%e7%8f%be%e5%9c%a8%e6%9c%80%e5%bc%b7%e3%81%ae%ef%bc%89%e3%82%bf%e3%83%b3%e3%83%91%e3%82%af%e8%b3%aa%e5%b1%80%e5%9c%a8%e3%83%87%e3%83%bc%e3%82%bf%e3%83%99%e3%83%bc%e3%82%b9","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3790","title":{"rendered":"\uff08\u73fe\u5728\u6700\u5f37\u306e\uff09\u30bf\u30f3\u30d1\u30af\u8cea\u5c40\u5728\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9"},"content":{"rendered":"<p>\u51fa\u82bd\u9175\u6bcd\uff08<em>S.cerevisiae<\/em>\uff09\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c40\u5728\u89e3\u6790\u306b\u3064\u3044\u3066\u306f\u3001USCF\uff08\u5f53\u6642\uff09\u306eErin O&#8217;Shea\u3089\u306e\u91d1\u5b57\u5854\u7684\u7814\u7a76\u304c\u6709\u540d\u3067\u3059\uff08\u6587\u732e\uff11\uff09\u3002\u51fa\u82bd\u9175\u6bcd\u306e\u307b\u307c\u3059\u3079\u3066\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092GFP\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u767a\u73fe\u3057\u3001\u305d\u308c\u3089\u306e\u5c40\u5728\u3092\u9855\u5fae\u93e1\u89b3\u5bdf\u3057\u305f\uff08\u305d\u308c\u3068\u540c\u6642\u306b\u767a\u73fe\u91cf\u3082\u6e2c\u3063\u305f\uff09\u3068\u3044\u3046\u3082\u306e\u3067\u3059\u3002\u3053\u306e\u30c7\u30fc\u30bf\u306f\u3001<a href=\"https:\/\/yeastgfp.yeastgenome.org\/index.php\" target=\"_blank\" rel=\"noopener\">YeastGFP<\/a>\u3068\u3044\u3046\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3067\u516c\u958b\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3053\u306e\u7814\u7a76\u306f\u3001\u5c40\u5728\u5316\u3068\u767a\u73fe\u91cf\u3092\u3088\u308a\u8a73\u7d30\u306b\u5b9a\u7fa9\u30fb\u5b9a\u91cf\u3057\u305f\u30c8\u30ed\u30f3\u30c8\u5927\u5b66\u306eBrenda Andrews\u306e\u30b0\u30eb\u30fc\u30d7\u306e\u7814\u7a76\u306b\u5f15\u304d\u7d99\u304c\u308c\u3001\u305d\u306e\u89e3\u6790\u7d50\u679c\u306f<a href=\"http:\/\/cyclops.ccbr.utoronto.ca\/\">CYCLoPs<\/a>\u3068\u3044\u3046\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3067\u516c\u958b\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u6587\u732e2\uff09\u3002\u4e0a\u8a18\u306e\u7814\u7a76\u306f\u3001\u57fa\u672c\u7684\u306b\u306fC\u672b\u7aef\u306bGFP\u3092\u4ed8\u52a0\u3057\u305f\u540c\u3058\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u7528\u3044\u3066\u884c\u308f\u308c\u305f\u7814\u7a76\u3067\u3059\u3002<\/p>\n<p>\u6700\u8fd1\u3001\u30cf\u30a4\u30c7\u30eb\u30d9\u30eb\u30af\u5927\u5b66\u306eMichael Knop\u3084\u30ef\u30a4\u30c4\u30de\u30f3\u7814\u7a76\u6240\u306eMaya Schuldiner\u3089\u304c\u958b\u767a\u3057\u305fSWAp-Tag strategy\uff08\u6587\u732e\uff13\uff09\u306b\u3088\u3063\u3066\u3001N\u672b\u7aef\u3068C\u672b\u7aef\u306b\u305d\u308c\u305e\u308c\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u4ed8\u52a0\u3055\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\uff08SWAT\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\uff09\u304c\u69cb\u7bc9\u3055\u308c\u3001\u3053\u308c\u3092\u7528\u3044\u305f\u5c40\u5728\u89b3\u5bdf\u306e\u7d50\u679c\u304c\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3068\u3057\u3066\u516c\u958b\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u306e<a href=\"http:\/\/shmoo.weizmann.ac.il\/elevy\/YeastRGB\/HTML\/YeastRGB.html\" target=\"_blank\" rel=\"noopener\">Yeast RGB<\/a>\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306f\u3001\u7d30\u80de\u5185\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c40\u5728\u304c\u3068\u3066\u3082\u898b\u3084\u3059\u304f\u3001\u73fe\u6642\u70b9\u3067\u306f\u6700\u5f37\u306e\u51fa\u82bd\u9175\u6bcd\u30bf\u30f3\u30d1\u30af\u8cea\u5c40\u5728\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3060\u308d\u3046\u3068\u601d\u3044\u307e\u3059\u3002\u4e0a\u8a18\u306e\u30aa\u30ea\u30b8\u30ca\u30ebC\u672b\u7aefGFP\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u52a0\u3048\u3001SWAT\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306eN\u672b\u7aef\u30fbC\u672b\u7aef\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c40\u5728\u3082\u5927\u304d\u306a\u7d30\u80de\u753b\u50cf\u3068\u3068\u3082\u306b\u793a\u3055\u308c\u3066\u3044\u3066\u3001\u3055\u3089\u306b\u5c40\u5728\u30de\u30fc\u30ab\u30fc\u3068\u306e\u91cd\u306d\u5408\u308f\u305b\u753b\u50cf\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u3061\u306a\u307f\u306b\u3001\u4e88\u60f3\u3055\u308c\u308b\u3053\u3068\u3067\u306f\u3042\u308a\u307e\u3059\u304c\u3001N\u672b\u7aef\u3068C\u672b\u7aef\u306b\u86cd\u5149\u30bf\u30b0\u3092\u7d50\u5408\u3057\u305f\u5834\u5408\u306b\u5c40\u5728\u304c\u7570\u306a\u3063\u3066\u898b\u3048\u308b\u4f8b\u3082\u591a\u6570\u89b3\u5bdf\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u7279\u306bN\u672b\u7aef\u3084C\u672b\u7aef\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c40\u5728\u5316\u30b7\u30b0\u30ca\u30eb\u304c\u3042\u308b\u3088\u3046\u306a\u8f38\u9001\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u306f\u9855\u8457\u306e\u3088\u3046\u3067\u3059\uff08\u6587\u732e\uff14\uff09\u3002<\/p>\n<p>\u30fc\u30fc<\/p>\n<p>\u6587\u732e<\/p>\n<ol>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/14562095\">Global analysis of protein localization in budding yeast.\u00a0<\/a>Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O&#8217;Shea EK.\u00a0<span class=\"jrnl\" title=\"Nature\">Nature<\/span>. 2003 Oct 16;425(6959):686-91.\u00a0PMID:14562095<\/li>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26048563\">CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae.\u00a0<\/a>Koh JL, Chong YT, Friesen H, Moses A, Boone C, Andrews BJ, Moffat J.\u00a0<span class=\"jrnl\" title=\"G3 (Bethesda, Md.)\">G3 (Bethesda)<\/span>. 2015 Apr 15;5(6):1223-32. doi: 10.1534\/g3.115.017830.\u00a0PMID:26048563<\/li>\n<li class=\"title\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26928762\">One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.\u00a0<\/a>Yofe I, Weill U, Meurer M, Chuartzman S, Zalckvar E, Goldman O, Ben-Dor S, Sch\u00fctze C, Wiedemann N, Knop M, Khmelinskii A, Schuldiner M.\u00a0<span class=\"jrnl\" title=\"Nature methods\">Nat Methods<\/span>. 2016 Apr;13(4):371-378. doi: 10.1038\/nmeth.3795. Epub 2016 Feb 29.\u00a0PMID:26928762<\/li>\n<li><a href=\"https:\/\/www.biorxiv.org\/content\/early\/2018\/07\/03\/360636\" target=\"_blank\" rel=\"noopener\">Systematic assessment of GFP tag position on protein localization and growth fitness in yeast\u00a0<\/a>Dan\u00a0Davidi,\u00a0Uri\u00a0Weill,\u00a0Gat\u00a0Krieger,\u00a0Zohar\u00a0Avihou, Ron\u00a0Milo, Maya\u00a0Schuldiner,\u00a0doi:\u00a0https:\/\/doi.org\/10.1101\/360636<\/li>\n<\/ol>\n<div class=\"aux\">\n<div class=\"resc\"><\/div>\n<\/div>\n<div class=\"aux\">\n<div class=\"resc\"><\/div>\n<\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u51fa\u82bd\u9175\u6bcd\uff08S.cerevisi&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3796,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[13,10,25,139,9],"tags":[],"class_list":["post-3790","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-13","category-10","category-25","category-139","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3790","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3790"}],"version-history":[{"count":6,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3790\/revisions"}],"predecessor-version":[{"id":3797,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/3790\/revisions\/3797"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3796"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3790"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3790"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3790"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}