{"id":4038,"date":"2020-06-08T14:04:35","date_gmt":"2020-06-08T05:04:35","guid":{"rendered":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=4038"},"modified":"2020-07-13T13:03:05","modified_gmt":"2020-07-13T04:03:05","slug":"%e3%82%b7%e3%83%aa%e3%83%bc%e3%82%ba%e9%81%8e%e5%89%b0%e7%99%ba%e7%8f%be%e3%83%bb%e7%ac%ac%ef%bc%95%e5%9b%9e%e3%80%8c%e9%81%8e%e5%89%b0%e7%99%ba%e7%8f%be%e3%81%ab%e7%94%a8%e3%81%84%e3%82%89%e3%82%8c","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=4038","title":{"rendered":"\u30b7\u30ea\u30fc\u30ba\u904e\u5270\u767a\u73fe\u30fb\u7b2c5\u56de\u300c\u904e\u5270\u767a\u73fe\u306b\u7528\u3044\u3089\u308c\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u300d"},"content":{"rendered":"\n<p>\u672c\u30a8\u30f3\u30c8\u30ea\u30fc\u3067\u306f\u3001\u51fa\u82bd\u9175\u6bcd\u306e\u904e\u5270\u767a\u73fe\u3067\u7528\u3044\u3089\u308c\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u306b\u3064\u3044\u3066\u3059\u3053\u3057\u8a73\u3057\u304f\u89e3\u8aac\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3082\u3044\u304f\u3064\u304b\u306b\u5206\u985e\u3067\u304d\u307e\u3059\u3002\u6052\u5e38\u7684\uff08\u69cb\u6210\u7684\uff09\u306b\u5f37\u304f\u767a\u73fe\u3059\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3001\u3053\u308c\u306f\u89e3\u7cd6\u7cfb\u9175\u7d20<em>TDH3\uff0f<\/em>\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3084\u30a2\u30af\u30c1\u30f3<em>ACT1<\/em>\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002<em>TDH3<\/em>\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08\u6b74\u53f2\u7684\u306b<em>GPD<\/em>\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3082\u547c\u3070\u308c\u308b\uff09\u306f\u9175\u6bcd\u306e\u4e2d\u3067\u306f\u6700\u5f37\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3067\u3059\u3002\u51fa\u82bd\u9175\u6bcd\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u5f37\u5ea6\u306b\u3064\u3044\u3066\u306f\u4ee5\u524d\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u306b\u66f8\u304d\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<figure class=\"wp-block-embed-wordpress wp-block-embed is-type-wp-embed is-provider-\u9175\u6bcd\u3068\u30b7\u30b9\u30c6\u30e0\u30d0\u30a4\u30aa\u30ed\u30b8\u30fc\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"kPeRIJk2Qj\"><a href=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=321\">\u51fa\u82bd\u9175\u6bcd\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u4f7f\u3048\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u7279\u6027\u8a55\u4fa1<\/a><\/blockquote><iframe class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; clip: rect(1px, 1px, 1px, 1px);\" title=\"&#8220;\u51fa\u82bd\u9175\u6bcd\u306e\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u4f7f\u3048\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u7279\u6027\u8a55\u4fa1&#8221; &#8212; \u9175\u6bcd\u3068\u30b7\u30b9\u30c6\u30e0\u30d0\u30a4\u30aa\u30ed\u30b8\u30fc\" src=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=321&#038;embed=true#?secret=oKVesRUUCT#?secret=kPeRIJk2Qj\" data-secret=\"kPeRIJk2Qj\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<p>\u6b21\u306b\u6761\u4ef6\u4f9d\u5b58\u7684\u306b\u5f37\u767a\u73fe\u304c\u8d77\u304d\u308b\u8a98\u5c0e\u6027\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3002\u51fa\u82bd\u9175\u6bcd\u3067\u306f\u5f8c\u306b\u8a73\u3057\u304f\u89e3\u8aac\u3059\u308bGAL\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u3002\u305d\u306e\u4ed6\u3001\u4eba\u5de5\u7684\u306b\u4f5c\u3089\u308c\u305fTet\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3084\u03b2\u30a8\u30b9\u30c8\u30e9\u30b8\u30aa\u30fc\u30eb\u8a98\u5c0e\u6027\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u51fa\u82bd\u9175\u6bcd\u3067\u306e\u30b7\u30b9\u30c6\u30de\u30c6\u30a3\u30c3\u30af\u306a\u904e\u5270\u767a\u73fe\u3067\u7528\u3044\u3089\u308c\u3066\u304d\u305f\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u3001\u307b\u307c <em>GAL<\/em>\u30d7\u30ed\u30e2\u30fc\u30bf\u306e\u4e00\u629e\u3067\u3059\u3002\u300c\u30ae\u30e3\u30eb\u300d\u3068\u8aad\u307f\u307e\u3059\u3002<em>GAL<\/em>\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u3001\u51fa\u82bd\u9175\u6bcd\u306e <em>GAL1<\/em>\u3068<em>GAL10<\/em>\u306e\u9593\u306b\u4f4d\u7f6e\u3057\u3066\u3044\u307e\u3059\u3002<em>GAL1<\/em>\u3068<em>GAL10<\/em>\u306f\u53cc\u65b9\u304c\u53cd\u5bfe\u5411\u304d\u306b\u306a\u308b\u3088\u3046\u306bORF\u304c\u914d\u7f6e\u3055\u308c\u3066\u304a\u308a\uff08\u56f35-1\uff09\u3001\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u306e\u4e2d\u9593\u9818\u57df\u3092\u7528\u3044\u308b\u3068\u3001\u53cc\u65b9\u5411\u306b\uff12\u3064\u540c\u6642\u306b\u907a\u4f1d\u5b50\u8ee2\u5199\u3092\u8a98\u5c0e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<em>GAL<\/em>\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306f\u3001\u57f9\u5730\u4e2d\u306e\u7cd6\u6e90\u3092\u30b0\u30eb\u30b3\u30fc\u30b9\u304b\u3089\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u306b\u5909\u3048\u308b\u3053\u3068\u3067\u8a98\u5c0e\u304c\u53ef\u80fd\u3067\u3059\u3002\u3053\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u306b\u3088\u308a\u99c6\u52d5\u3055\u308c\u308b\u767a\u73fe\u306f\u3001\u30b0\u30eb\u30b3\u30fc\u30b9\u57f9\u5730\u3067\u306f\u30b0\u30eb\u30b3\u30fc\u30b9\u6291\u5236\u3068\u3044\u3046\u73fe\u8c61\u306b\u3088\u308a\u6291\u3048\u3089\u308c\u3066\u304a\u308a\u3001\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u57f9\u5730\u3067\u5f37\u304f\u8a98\u5c0e\u3055\u308c\u307e\u3059\u3002\u3053\u306eON\uff0fOFF\u306e\u30b7\u30e3\u30fc\u30d7\u3055\u304c\u3053\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u597d\u307e\u308c\u308b\u7406\u7531\u3067\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-style-default\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"411\" src=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/dcfb43398d4a5ae7d38057832c10ea92-1024x411.png\" alt=\"\" class=\"wp-image-4034\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/dcfb43398d4a5ae7d38057832c10ea92-1024x411.png 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/dcfb43398d4a5ae7d38057832c10ea92-300x120.png 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/dcfb43398d4a5ae7d38057832c10ea92-768x308.png 768w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/dcfb43398d4a5ae7d38057832c10ea92-1536x617.png 1536w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/dcfb43398d4a5ae7d38057832c10ea92-956x384.png 956w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/dcfb43398d4a5ae7d38057832c10ea92.png 1898w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>\u56f35-1. S. cerevisiae\u306eGAL1 &#8211; GAL10\u907a\u4f1d\u5b50\u9818\u57df\u3002\u3053\u308c\u3089\u306e\u9593\u306b\u53cc\u65b9\u5411\u30d7\u30ed\u30e2\u30fc\u30bf\u306b\u30d7\u30ed\u30e2\u30fc\u30bf\u304c\u3042\u308b\u3002<\/figcaption><\/figure>\n\n\n\n<p>\u307e\u305f\u3001GAL1\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u767a\u73fe\u5f37\u5ea6\u3092\u5909\u3048\u305f\uff08\u4e0b\u3052\u305f\uff09\u6539\u5909\u4f53\u3082\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\uff08<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/7838736\/\">Mumberg et ., 1994<\/a>\uff09\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u904e\u5270\u767a\u73fe\u306e\u5ea6\u5408\u3044\u3092\u5909\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u305f\u3060\u3001\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u3078\u306e\u57f9\u5730\u5909\u63db\u3092\u884c\u3046\u5fc5\u8981\u304c\u3042\u308b\u3053\u3068\u3001\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u306e\u8a98\u5c0e\u306b\u3088\u308a\u6a19\u7684\u907a\u4f1d\u5b50\u4ee5\u5916\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3082\u8a98\u5c0e\u3055\u308c\u3001\u305d\u308c\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u7cfb\u306b\u4f59\u8a08\u306a\u8ca0\u8377\u3092\u304b\u3051\u308b\u3053\u3068\u306a\u3069\u304c\u61f8\u5ff5\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u305d\u308c\u3067\u3053\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u4eba\u5de5\u8ee2\u5199\u56e0\u5b50\u3067\u8a8d\u8b58\u3067\u304d\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08\u03b2\u30a8\u30b9\u30c8\u30e9\u30b8\u30aa\u30fc\u30eb\u8a98\u5c0e\u6027\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff09\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306b\u3064\u3044\u3066\u306f\u3001\u7b46\u8005\u306e\u4ee5\u524d\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u3067\u8aac\u660e\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-embed-wordpress wp-block-embed is-type-wp-embed is-provider-\u9175\u6bcd\u3068\u30b7\u30b9\u30c6\u30e0\u30d0\u30a4\u30aa\u30ed\u30b8\u30fc\"><div class=\"wp-block-embed__wrapper\">\n<blockquote class=\"wp-embedded-content\" data-secret=\"OHo3uCBxMH\"><a href=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=3104\">\u30db\u30eb\u30e2\u30f3\u3092\u5229\u7528\u3057\u305f\u65b0\u3057\u3044\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u30b7\u30b9\u30c6\u30e0<\/a><\/blockquote><iframe class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; 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clip: rect(1px, 1px, 1px, 1px);\" title=\"&#8220;\u5206\u88c2\u9175\u6bcd\u3067\u201c\u4f7f\u3048\u308b\u201d\u8a98\u5c0e\u578b\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304c\u898b\u3064\u304b\u308b\u306e\u306f\u3044\u3064\u306e\u65e5\u304b\uff1f&#8221; &#8212; \u9175\u6bcd\u3068\u30b7\u30b9\u30c6\u30e0\u30d0\u30a4\u30aa\u30ed\u30b8\u30fc\" src=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=528&#038;embed=true#?secret=d5qgOKruOl#?secret=Co2E75qFMU\" data-secret=\"Co2E75qFMU\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<p>\u306a\u304a\u3001\u5c11\u3005\u53e4\u3044\u672c\u306b\u306a\u308a\u307e\u3059\u304c\u3001\u9175\u6bcd\u3067\u7570\u7a2e\u767a\u73fe\u306b\u4f7f\u3048\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306b\u95a2\u3057\u3066\u306f\u3001Guide to Yeast Genetics and Molecular and Cell Biology (Part B\uff09\u306e [14] &#8220;Vector Systems for Heterologous Expression of Proteins in<em> Saccharomyces cerevisiae<\/em>&#8221; 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