{"id":4081,"date":"2020-06-12T10:56:03","date_gmt":"2020-06-12T01:56:03","guid":{"rendered":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=4081"},"modified":"2020-08-04T14:24:51","modified_gmt":"2020-08-04T05:24:51","slug":"%e3%82%b7%e3%83%aa%e3%83%bc%e3%82%ba%e9%81%8e%e5%89%b0%e7%99%ba%e7%8f%be%e3%83%bb%e7%ac%ac8%e5%9b%9e%e3%80%8c%e9%81%ba%e4%bc%9d%e5%ad%90%e3%83%bborf%e3%81%af%e3%81%a9%e3%81%ae%e3%82%88%e3%81%86","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=4081","title":{"rendered":"\u30b7\u30ea\u30fc\u30ba\u904e\u5270\u767a\u73fe\u30fb\u7b2c8\u56de\u300c\u907a\u4f1d\u5b50\u30fbORF\u306f\u3069\u306e\u3088\u3046\u306b\u6c7a\u3081\u3089\u308c\u308b\u304b\uff1f\u300d"},"content":{"rendered":"\n<p>\u524d\u56de\u307e\u3067\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u3067\u3001\u30dd\u30b9\u30c8\u30b2\u30ce\u30e0\u306e\u30b7\u30b9\u30c6\u30de\u30c6\u30a3\u30c3\u30af\u306a\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u89e3\u6790\u3001\u7279\u306b\u907a\u4f1d\u5b50\u7834\u58ca\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3084\u904e\u5270\u767a\u73fe\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u306f\u3001\u30b2\u30ce\u30e0\u306e\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3092\u30d9\u30fc\u30b9\u306b\u907a\u4f1d\u5b50\u3092\u8abf\u3079\u3066\u3044\u304f\u3068\u89e3\u8aac\u3057\u307e\u3057\u305f\u3002\u672c\u30a8\u30f3\u30c8\u30ea\u30fc\u3067\u306f\u3001\u305d\u306e\u300c\u907a\u4f1d\u5b50\uff08\u3042\u308b\u3044\u306fORF\uff09\u300d\u3068\u3044\u3046\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u306f\u3069\u306e\u3088\u3046\u306b\u6c7a\u3081\u3089\u308c\u3066\u304d\u305f\u306e\u304b\u3092\u8003\u3048\u3066\u307f\u305f\u3044\u3068\u601d\u3044\u307e\u3059<\/p>\n\n\n\n<p>\u30b2\u30ce\u30e0\u306e\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3068\u306f\u3001\u672c\u30a8\u30f3\u30c8\u30ea\u30fc\u5f8c\u534a\u3067\u7d39\u4ecb\u3059\u308b\u3088\u3046\u306a\u69d8\u3005\u306a\u60c5\u5831\u3092\u3082\u3068\u306b\u3001\u300c\u30b2\u30ce\u30e0DNA\u4e0a\u306e\u3069\u306e\u4f4d\u7f6e\u306b\u3069\u306e\u3088\u3046\u306a\u56e0\u5b50\u304c\u5b58\u5728\u3059\u308b\u304b\u300d\u3001\u3068\u3044\u3046\u6ce8\u91c8\u306e\u3053\u3068\u3092\u6307\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001\u56f38-1\u3067\u306f\u3001<em>S. cerevisiae<\/em>\u30b2\u30ce\u30e0\u306e\u7279\u5b9a\u306e\u9818\u57df\uff08\u7b2cVI\u67d3\u8272\u306e<em>ACT1<\/em>\u304c\u5b58\u5728\u3059\u308b\u9818\u57df\uff09\u306b\u304a\u3044\u3066\u306e\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3067\u306f\u907a\u4f1d\u5b50\u306e\u60c5\u5831\u306e\u307f\u3001\u305d\u306e\u9818\u57df\u3067\u3069\u3061\u3089\u5411\u304d\u306b\u3069\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u304c\u5b58\u5728\u3057\u3066\u3044\u308b\u304b\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-style-default\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"229\" src=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/1845697f8620707763b98fe208b7d0b6-1024x229.png\" alt=\"\" class=\"wp-image-4085\" srcset=\"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/1845697f8620707763b98fe208b7d0b6-1024x229.png 1024w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/1845697f8620707763b98fe208b7d0b6-300x67.png 300w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/1845697f8620707763b98fe208b7d0b6-768x172.png 768w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/1845697f8620707763b98fe208b7d0b6-1536x343.png 1536w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/1845697f8620707763b98fe208b7d0b6-2048x458.png 2048w, https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/wp-content\/uploads\/2020\/06\/1845697f8620707763b98fe208b7d0b6-956x214.png 956w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>\u56f38-1. S. cerevisiae\u30b2\u30ce\u30e0\u3001ACT1\u9818\u57df\u306e\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\uff08ORF\u60c5\u5831\u306e\u307f\uff09\u3002Saccharomyces Genome Database\u3088\u308a<\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">\u907a\u4f1d\u5b50\u306e\u5b9a\u7fa9\u3001ORF\u306e\u5b9a\u7fa9<\/h3>\n\n\n\n<p>\u5c11\u3057\u3053\u3053\u3067\u6a2a\u9053\u306b\u305d\u308c\u3066\u3001\u300c\u907a\u4f1d\u5b50\u300d\u3068\u3044\u3046\u3082\u306e\u306e\u5b9a\u7fa9\u306b\u3064\u3044\u3066\u8003\u3048\u3066\u307f\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u53e4\u5178\u7684\u306a\u907a\u4f1d\u5b66\u306b\u304a\u3044\u3066\u3001\u907a\u4f1d\u5b50\u3068\u306f\u3001\u300c\u5b50\u5b6b\u306b\u53d7\u3051\u7d99\u304c\u308c\u308b\u5f62\u8cea\u306e\u60c5\u5831\u3092\u542b\u3093\u3060\u56e0\u5b50\u300d\u306e\u3053\u3068\u3092\u610f\u5473\u3057\u3066\u3044\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u907a\u4f1d\u5b50\u306e\u5b9f\u614b\u304c\u3042\u304d\u3089\u304b\u306b\u306a\u308b\u4ee5\u524d\u306e\u3053\u3068\u3067\u3059\u3002\u73fe\u5728\u3001\u907a\u4f1d\u5b50\u306e\u5b9f\u614b\u306fDNA\u306e\u7279\u5b9a\u306e\u9818\u57df\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u305d\u308c\u3067\u306f\u3001\u907a\u4f1d\u5b50\u3068\u306f\u5177\u4f53\u7684\u306bDNA\u4e0a\u306e\u3069\u3053\u304b\u3089\u3069\u3053\u307e\u3067\u3092\u6307\u3059\u306e\u3067\u3057\u3087\u3046\u304b\uff1f \u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30b3\u30fc\u30c9\u3059\u308b\u9818\u57df\uff1f mRNA\u3068\u3057\u3066\u8ee2\u5199\u3055\u308c\u308b\u9818\u57df\uff1f \u3042\u308b\u3044\u306f\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u8a98\u5c0e\u3059\u308b\u9818\u57df\u307e\u3067\u542b\u3080\uff1f\u3053\u3046\u3044\u3046\u5b9a\u7fa9\u306f\u3001\u5404\u7814\u7a76\u8005\u304c\u30d0\u30e9\u30d0\u30e9\u306b\u81ea\u5206\u306e\u597d\u304d\u306a\u907a\u4f1d\u5b50\u3092\u7814\u7a76\u3057\u3066\u3044\u305f\u9803\u306b\u306f\u66d6\u6627\u3067\u3082\u3088\u304b\u3063\u305f\u3067\u3057\u3087\u3046\u3002\u79c1\u306e\u624b\u5143\u306b\u3042\u308b\u6559\u79d1\u66f8\u3067\u306f\u3001\u300cmRNA\u3068\u3057\u3066\u8ee2\u5199\u3055\u308c\u308b\u9818\u57df\u3092\u907a\u4f1d\u5b50\u3068\u547c\u3076\u3053\u3068\u306b\u3059\u308b\u300d\u3068\u3042\u308a\u307e\u3059\u3002\u305f\u3060\u3001\u73fe\u5728\u306e\u7814\u7a76\u304b\u3089mRNA\u3068\u3057\u3066\u8ee2\u5199\u3055\u308c\u308b\u9818\u57df\u3082\u304d\u3061\u3093\u3068\u6c7a\u307e\u3063\u3066\u3044\u306a\u304f\u3066\u3001\u3042\u308b\u7a0b\u5ea6\u306e\u63fa\u3089\u304e\u304c\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u30b2\u30ce\u30e0\u3068\u3044\u3046\u7d71\u4e00\u7684\u306a\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u60c5\u5831\u3092\u6271\u3046\u306b\u306f\u3001\u304d\u3061\u3093\u3068\u3057\u305f\u5b9a\u7fa9\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u3067\u306a\u3044\u3068\u3001\u300c\u30b2\u30ce\u30e0\u4e0a\u306e\u3053\u306e\u5869\u57fa\u304b\u3089\u3053\u306e\u5869\u57fa\u307e\u3067\u304cxxx\u3068\u3044\u3046\u907a\u4f1d\u5b50\u3067\u3059\u300d\u3068\u3044\u3046\u6ce8\u91c8\u304c\u6210\u308a\u7acb\u305f\u306a\u3044\u304b\u3089\u3067\u3059\u3002\u3068\u3044\u3046\u308f\u3051\u3067\u3001\u5b9a\u7fa9\u304c\u66d6\u6627\u306a\u300c\u907a\u4f1d\u5b50\u300d\u3068\u3044\u3046\u8a00\u8449\u306f\u3001\u30b2\u30ce\u30e0\u306e\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3067\u306f\u4f7f\u3044\u307e\u305b\u3093\u3002\u305d\u306e\u304b\u308f\u308aATG\u304b\u3089STOP\u30b3\u30c9\u30f3\u306b\u306f\u631f\u307e\u308c\u305fDNA\u9818\u57df\u3092\u300cOpen Reading Frame 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