{"id":557,"date":"2013-01-29T15:33:45","date_gmt":"2013-01-29T06:33:45","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=557"},"modified":"2013-01-29T15:33:45","modified_gmt":"2013-01-29T06:33:45","slug":"rnaseq%e3%81%a8%e3%83%9e%e3%82%a4%e3%82%af%e3%83%ad%e3%82%a2%e3%83%ac%e3%82%a4%e3%81%ab%e3%82%88%e3%82%8bmrna%e3%81%ae%e5%ae%9a%e9%87%8f%e3%83%bc%e3%81%82%e3%82%8b%e8%a7%a3%e6%9e%90%e4%be%8b","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=557","title":{"rendered":"RNAseq\u3068\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u3088\u308bmRNA\u306e\u5b9a\u91cf\u30fc\u3042\u308b\u89e3\u6790\u4f8b"},"content":{"rendered":"

\u5225\u4ef6\u3067\u3001\u81ea\u524d\u3067\u3068\u3063\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u3088\u308bmRNA\u306e\u767a\u73fe\u91cf\u306e\u89e3\u6790\u30c7\u30fc\u30bf\u3068\u3001\u30d1\u30d6\u30ea\u30c3\u30b7\u30e5\u3055\u308c\u3066\u3044\u308bRNAseq\u306b\u3088\u308b\u540c\u69d8\u306a\u30c7\u30fc\u30bf\u3092\u6bd4\u8f03\u3059\u308b\u6a5f\u4f1a\u304c\u3042\u3063\u305f\u306e\u3067\u3001\u899a\u3048\u66f8\u304d\u306e\u610f\u5473\u3082\u542b\u3081\u3066\u30d6\u30ed\u30b0\u306b\u66f8\u3044\u3066\u304a\u304d\u307e\u3059\u3002<\/p>\n

\u307e\u305a\u3001\u6761\u4ef6\u3067\u3059\u304c\u3001\u79c1\u305f\u3061\u306f\u5408\u6210\u57f9\u5730\u3067\u3068\u3042\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u6301\u305f\u305b\u305f\u9175\u6bcd\u682a\u3092\u5bfe\u6570\u5897\u6b96\u671f\u307e\u3067\u5897\u6b96\u3055\u305b\u305f\u6761\u4ef6\u3067\u3001RNA\u3092\u62bd\u51fa\u3057\u3001\u30a2\u30b8\u30ec\u30f3\u30c8\u306eYeast\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a44x44k \uff081\u8272\u6cd5\uff09\u3067\u89e3\u6790\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u6bd4\u8f03\u5bfe\u8c61\u306e\uff11\u3064\u76ee\u306e\u8ad6\u6587\u3067\u306f\u3001\u5bcc\u6804\u990a\u57f9\u5730\uff08YPD\uff09\u3067\u5bfe\u6570\u5897\u6b96\u671f\u307e\u3067\u5897\u6b96\u3055\u305b\u305f\u7d30\u80de\u3092RNAseq\u3067\u89e3\u6790\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

The transcriptional landscape of the yeast genome defined by RNA sequencing.<\/a>\u00a0Nagalakshmi\u00a0U, Wang Z, Waern K, Shou C, Raha D, Gerstein M,\u00a0Snyder\u00a0M. Science. 2008 Jun 6;320(5881):1344-9. doi: 10.1126\/science.1158441. Epub 2008 May 1.\u00a0PMID:18451266<\/p>\n

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\n

\"\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8<\/a><\/p>\n

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\u3057\u304b\u3057\u6c17\u306b\u306a\u308b\u306e\u304c\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u91cf\u304c\u4f4e\u3044\u907a\u4f1d\u5b50\u3002RNAseq\u3067\u306f2\u3088\u308a\u4e0b\u3067\u306f\u3001\u30d7\u30ed\u30c3\u30c8\u304c\u6a2a\u306b\u4e26\u3093\u3067\u3057\u307e\u3063\u3066\u304a\u308a\u3001\u89e3\u50cf\u5ea6\u304c\u60aa\u304f\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u3053\u306e\u89e3\u6790\u3067\u306f\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u89e3\u6790\u3067\u691c\u51fa\u3067\u304d\u3066\u3044\u308b900\u500b\u8fd1\u3044\u907a\u4f1d\u5b50\u306e\u89e3\u6790\u304c\u3067\u304d\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n

\u6b21\u306b\u3001\u540c\u3058\u89e3\u6790\u3092\u3057\u305f\u5225\u306e\u8ad6\u6587\u306e\u30c7\u30fc\u30bf\u3092\u898b\u3066\u307f\u307e\u3059\u3002<\/p>\n

2\u3064\u76ee\u306e\u8ad6\u6587\u3067\u3082\u3001\u5bcc\u6804\u990a\u57f9\u5730\uff08YPD\uff09\u3067\u5bfe\u6570\u5897\u6b96\u671f\u307e\u3067\u5897\u6b96\u3055\u305b\u305f\u7d30\u80de\u3092RNAseq\u3067\u89e3\u6790\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

Ab initio construction of a eukaryotic transcriptome by massively parallel mRNA sequencing.<\/a>\u00a0Yassour M, Kaplan T, Fraser HB, Levin JZ, Pfiffner J, Adiconis X, Schroth G, Luo S, Khrebtukova I, Gnirke A, Nusbaum C, Thompson DA, Friedman N, Regev A. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3264-9. Doi:10.1073\/pnas.0812841106. Epub 2009 Feb 10.\u00a0PMID:19208812<\/p>\n

\"\u30b9\u30af\u30ea\u30fc\u30f3\u30b7\u30e7\u30c3\u30c8<\/a><\/p>\n

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\u5f8c\u304b\u3089\u767a\u8868\u3055\u308c\u3066\u3044\u308b\u8ad6\u6587\u3067\u3059\u304b\u3089\u3001\u30c7\u30fc\u30bf\u306e\u4e2d\u8eab\u304c\u826f\u304f\u306a\u308b\u306e\u306f\u5f53\u7136\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u30022013\u5e74\u306e\u73fe\u5728\u3067\u306f\u3082\u3063\u3068\u8cea\u306e\u9ad8\u3044\u30c7\u30fc\u30bf\u304c\u3067\u308b\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n

\u3053\u306e\u30a8\u30f3\u30c8\u30ea\u30fc\u306e\u7d50\u8ad6\u3068\u3057\u3066\u30012\u3064\u306e\u3053\u3068\u3092\u3044\u3044\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\uff11\uff09\u8ab0\u304b\u304c\u30d1\u30d6\u30ea\u30c3\u30b7\u30e5\u3057\u305f\u5927\u898f\u6a21\u30c7\u30fc\u30bf\u3092\u4f7f\u3046\u3068\u304d\u306b\u306f\u3001\u30c7\u30fc\u30bf\u306e\u8cea\u306b\u6ce8\u610f\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002Science\u306e\u30c7\u30fc\u30bf > PNAS\u306e\u30c7\u30fc\u30bf\u3067\u306f\u306a\u3044\u3002\u7686\u304c\u4f7f\u3063\u3066\u3044\u308b\u304b\u3089\u3068\u3044\u3063\u3066\u3001\u305d\u308c\u3092\u4f7f\u3063\u305f\u3089\u826f\u3044\u8a33\u3067\u306f\u306a\u3044\u3002<\/p>\n

\uff12\uff09\u73fe\u5728\u306e\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306b\u3088\u308bmRNA\u306e\u767a\u73fe\u91cf\u89e3\u6790\u306e\u89e3\u50cf\u5ea6\u306f\u304b\u306a\u308a\u9ad8\u3044\u3002<\/p>\n

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