{"id":97,"date":"2011-04-28T12:03:45","date_gmt":"2011-04-28T03:03:45","guid":{"rendered":"http:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=97"},"modified":"2017-08-10T16:43:37","modified_gmt":"2017-08-10T07:43:37","slug":"%e5%87%ba%e8%8a%bd%e9%85%b5%e6%af%8d%e3%81%a8%e5%88%86%e8%a3%82%e9%85%b5%e6%af%8d%e3%81%ae%e5%9f%ba%e6%9c%ac%e7%9f%a5%e8%ad%98","status":"publish","type":"post","link":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/?p=97","title":{"rendered":"\u51fa\u82bd\u9175\u6bcd\u3068\u5206\u88c2\u9175\u6bcd\u306e\u57fa\u672c\u77e5\u8b58"},"content":{"rendered":"<p>\u51fa\u82bd\u9175\u6bcd\u00a0<em>Saccharomyces cerevisiae<\/em> \u3068\u5206\u88c2\u9175\u6bcd\u00a0<em>Schizosaccharomyces pombe<\/em> \u306f2\u3064\u306e\u5927\u5909\u7570\u306a\u3063\u305f\u30bf\u30a4\u30d7\u306e\u9175\u6bcd\u3067\u3001\u771f\u6838\u7d30\u80de\u306e\u30e2\u30c7\u30eb\u7d30\u80de\u3068\u3057\u3066\u5e83\u304f\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u3053\u308c\u3089\u306e\u9175\u6bcd\u306e\u57fa\u790e\u77e5\u8b58\u306b\u3064\u3044\u3066\u307e\u3068\u3081\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<p><!--more-->\u5206\u5b50\u7cfb\u7d71\u5b66\u7684\u306a\u6839\u62e0\u304b\u3089\u3053\u308c\u3089\u306f10\u5104\u5e74\u4ee5\u4e0a\u6614\u306b\u5206\u5c90\u3057\u305f\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002<em>S. cerevisiae<\/em> \u306f\u305d\u306e\u5206\u5c90\u306e\u5f8c\u3001<em>S. pombe<\/em> \u3088\u308a\u3082\u65e9\u304f\u300c\u9032\u5316\u3057\u305f\u300d\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002<\/p>\n<p><em>S. pombe<\/em>\u306f\u3001\u5206\u88c2\u69d8\u5f0f\u3001\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u3001\u30a4\u30f3\u30c8\u30ed\u30f3\u3001\u30d8\u30c6\u30ed\u30af\u30ed\u30de\u30c1\u30f3\u306a\u3069\u304c\u3001<em>S. cerevisiae<\/em> \u3088\u308a\u3082\u52d5\u7269\u7d30\u80de\u306b\u4f3c\u3066\u3044\u308b\u3002\u4e00\u65b9\u3001G1\u671f\u3068G2\u671f\u306e\u9577\u3055\u3084\u30b7\u30ca\u30d7\u30c8\u30cd\u30de\u69cb\u9020\u306a\u3069\u306f<em> S. cerevisiae<\/em> \u306e\u65b9\u304c\u3088\u308a\u52d5\u7269\u7d30\u80de\u306b\u8fd1\u3044\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002<\/p>\n<div><span style=\"color: #444444; font-size: 20px; line-height: 30px;\">\u9175\u6bcd\u304c\u771f\u6838\u7d30\u80de\u306e\u512a\u308c\u305f\u30e2\u30c7\u30eb\u751f\u7269\u3067\u3042\u308b\u7406\u7531<\/span><\/div>\n<ul>\n<li>\u5b9f\u9a13\u5ba4\u3067\u7c21\u5358\u306b\u64cd\u4f5c\u3067\u304d\u308b\u3002<\/li>\n<li>\u5897\u6b96\u304c\u65e9\u3044\uff081.5\u304b\u30892.5\u6642\u9593\uff09\u3002<\/li>\n<li>\u75c5\u539f\u6027\u304c\u306a\u3044\u3002<\/li>\n<li>2\u500d\u4f53\uff08cerevisiae\uff09\u30681\u500d\u4f53\uff08\u4e21\u8005\uff09\u72b6\u614b\u304c\u5b89\u5b9a\u3057\u3066\u7dad\u6301\u3055\u308c\u308b\u3002<\/li>\n<li>1\u500d\u4f53\u304c\u7c21\u5358\u306b\u63a5\u5408\u3057\u30662\u500d\u4f53\u306b\u306a\u308b\u3002\n<ul>\n<li>\u76f8\u88dc\u6027\u30c6\u30b9\u30c8\u304c\u5bb9\u6613\u3067\u3042\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u5916\u90e8DNA\u3092\u52b9\u7387\u3088\u304f\u5f62\u8cea\u8ee2\u63db\u3059\u308b\u3002\n<ul>\n<li>\u30a4\u30f3\u30c6\u30b0\u30ec\u30fc\u30b7\u30e7\u30f3\uff08\u30b2\u30ce\u30e0\u3078\u306e\u7d44\u307f\u8fbc\u307f\uff09<\/li>\n<li>\u81ea\u5f8b\u8907\u88fd\u30d7\u30e9\u30b9\u30df\u30c9<\/li>\n<\/ul>\n<\/li>\n<li>\u76f8\u540c\u7d44\u307f\u63db\u3048\u52b9\u7387\u304c\u9ad8\u3044\u3002\n<ul>\n<li>\u30b2\u30ce\u30e0\u3078\u306e\u306d\u3089\u3063\u305f\u3068\u304a\u308a\u306e\u5909\u7570\u306e\u5c0e\u5165\u304c\u7c21\u4fbf\u3067\u3042\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<li>\u907a\u4f1d\u7684\u9078\u629e\u304c\u7c21\u4fbf\u3067\u3042\u308b\u3002<\/li>\n<\/ul>\n<h3 id=\"content_1_2\">\u7d30\u80de\u306e\u5927\u304d\u3055\u3068\u898b\u305f\u76ee<\/h3>\n<ul>\n<li><em>S. cerevisiae<\/em>\n<ul>\n<li>\u6955\u5186\u5f62\uff1b \u76f4\u5f84\u7d045 \u00b5m<\/li>\n<li>\u51fa\u82bd\u306b\u3088\u3063\u3066\u5897\u6b96\u3059\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li><em>S. pombe<\/em>\n<ul>\n<li>\u5186\u7b52\u5f62\uff1b \u76f4\u5f843-4 \u00b5m; \u9577\u30557-15 \u00b5m<\/li>\n<li>\u4e2d\u592e\u3067\u5206\u88c2\u3057\u3066\u5897\u6b96\u3059\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3 id=\"content_1_3\">\u751f\u6d3b\u74b0<\/h3>\n<ul>\n<li><em>S. cerevisiae<\/em>\u306f1\u500d\u4f53\u30682\u500d\u4f53\u306e\u5f62\u614b\u3067\u5b58\u5728\u3059\u308b\u30021\u500d\u4f53\u306b\u306f2\u3064\u306e\u6027\uff08a and alpha\uff09\u304c\u3042\u308b\u3002<\/li>\n<li><em>S. pombe<\/em>\u306f\u57fa\u672c\u7684\u306b\u306f1\u500d\u4f53\uff08h+\u3068h-\u306e\u6027\u304c\u3042\u308b\uff09\u3067\u5b58\u5728\u3059\u308b\u304c\u3001\u63a5\u5408\u306b\u3088\u3063\u3066\u4e00\u6642\u7684\u306b2\u500d\u4f53\u3068\u306a\u308b\u3002\u901a\u5e38\u306e\u6761\u4ef6\u3067\u306f\u30012\u500d\u4f53\u7d30\u80de\u306f\u5373\u5ea7\u306b\u6e1b\u6570\u5206\u88c2\u3092\u8d77\u3053\u3057\u30661\u500d\u4f53\u306e\u80de\u5b50\u3092\u5f62\u6210\u3059\u308b\u3002<\/li>\n<li><em>S. pombe<\/em>\u306e\u5b50\u56a2\u306f\u7dda\u72b6\u306a\u306e\u306b\u5bfe\u3057\u3066\u3001<em>S. cerevisiae<\/em>\u306e\u5b50\u56a2\u306f\u30c6\u30c8\u30e9\u30dd\u30c3\u30c8\u72b6\u3067\u3042\u308b\u3002<em>S. cerevisiae<\/em>\u306e\u30c6\u30c8\u30e9\u30dd\u30c3\u30c8\u72b6\u306e\u5b50\u56a2\u306f\u6e1b\u6570\u5206\u88c2\u306e\u9806\u756a\u306e\u60c5\u5831\u3092\u4fdd\u6301\u3057\u3066\u3044\u306a\u3044\u3002\u3057\u304b\u3057<em>S.pombe<\/em>\u306e\u76f4\u7dda\u306b\u4e26\u3093\u3060\u5b50\u56a2\u306f\u3053\u306e\u60c5\u5831\u3092\u4fdd\u6301\u3057\u3066\u3044\u308b\u3002<\/li>\n<li>\u6027\u306e\u5909\u63db\uff1a\u30db\u30e2\u30bf\u30ea\u30ba\u30e0\u3068\u30d8\u30c6\u30ed\u30bf\u30ea\u30ba\u30e0\n<ul>\n<li>\u4e21\u9175\u6bcd\u3067\u306e\u6027\u8ee2\u63db\u306e\u6a5f\u69cb\u306f\u975e\u5e38\u306b\u9762\u767d\u3044\u7814\u7a76\u6210\u679c\u3092\u3042\u3052\u3066\u3044\u308b\u3002<\/li>\n<li>\u91ce\u751f\u682a\u3067\u898b\u3089\u308c\u308b\u6027\u8ee2\u63db<\/li>\n<li>\u30d8\u30c6\u30ed\u30af\u30ed\u30de\u30c1\u30f3\u5316<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<div><span style=\"color: #444444; font-size: 20px; line-height: 30px;\">\u7d30\u80de\u5468\u671f<\/span><\/div>\n<ul>\n<li><em>S. cerevisiae<\/em>\n<ul>\n<li>G1\u671f\u5f8c\u671f\u306b\u30b9\u30d4\u30f3\u30c9\u30eb\u6975\u4f53\u306e\u8907\u88fd\u304c\u304a\u304d\u3001S\u671f\u4e2d\u306b\u30b9\u30d4\u30f3\u30c9\u30eb\u306e\u4f38\u9577\u304c\u304a\u304d\u308b\u3002\u5f93\u3063\u3066<em>S. cerevisiae<\/em>\u306eM\u671f\u306fG1\u3068S\u3068\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u3057\u3066\u304a\u308a\u3001\u306f\u3063\u304d\u308a\u3068\u3057\u305fG2\u671f\u304c\u898b\u3089\u308c\u306a\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<li><em>S. pombe<\/em>\n<ul>\n<li>\u30b9\u30d4\u30f3\u30c9\u30eb\u6975\u4f53\u306f\u307b\u3068\u3093\u3069\u306e\u4ed6\u306e\u771f\u6838\u751f\u7269\u306e\u3088\u3046\u306b\u3001\u6838\u5206\u88c2\u306e\u76f4\u524d\u306b\u304a\u304d\u308b\u3002\u3057\u304b\u3057\u6025\u901f\u306b\u5897\u6b96\u3057\u3066\u3044\u308b<em>S. pombe<\/em>\u3067\u306f\u7d30\u80de\u8cea\u5206\u88c2\u304c\uff08\u6b21\u306e\uff09S\u671f\u307e\u3067\u304a\u304d\u306a\u3044\u3002\u5f93\u3063\u3066\u6025\u901f\u306b\u5897\u6b96\u3057\u3066\u3044\u308b\u72b6\u614b\u3067\u306e\u4e00\u756a\u5c0f\u3055\u306a<em>S. pombe<\/em>\u306e\u7d30\u80de\u306f\u521d\u671fG2\u306b\u3042\u308b\u3002\u307b\u3068\u3093\u3069\u306e<em>S.pombe<\/em>\u7d30\u80de\u306e\u5897\u6b96\u306f\u3001<em>S.pombe<\/em>\u306e\u7d30\u80de\u5468\u671f\u306e\u307b\u3068\u3093\u3069\u3092\u3057\u3081\u308bG2\u671f\u306b\u304a\u304d\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<div><span style=\"color: #444444; font-size: 20px; line-height: 30px;\">\u4ee3\u8b1d<\/span><\/div>\n<ul>\n<li>\u4e21\u751f\u7269\u306f\u30b0\u30eb\u30b3\u30fc\u30b9\u3067\u6700\u3082\u65e9\u304f\u5897\u6b96\u3059\u308b\u3002\u30b0\u30eb\u30b3\u30fc\u30b9\u306f\u767a\u9175\u306b\u3088\u3063\u3066\u30a8\u30bf\u30ce\u30fc\u30eb\u306b\u306a\u308b\u304b\u9178\u5316\u3092\u3046\u3051\u3066CO2\u3068H2O\u306b\u306a\u308b\u3002<\/li>\n<li><em>S. cerevisiae<\/em>\u306f\u767a\u9175\u3092\u597d\u307f\u3001\u9178\u7d20\u304c\u5b58\u5728\u3057\u3066\u3044\u3088\u3046\u3068\u3082\u30b0\u30eb\u30b3\u30fc\u30b9\u304c\u3042\u308b\u9650\u308a\u767a\u9175\u3092\u7d9a\u3051\u308b\u3002<\/li>\n<li><em>S. pombe<\/em>\u306f\u9178\u7d20\u304c\u306a\u3044\u3068\u304d\u306e\u307f\u7d14\u7c8b\u306a\u767a\u9175\u3092\u884c\u3046\u3002<\/li>\n<li><em>S. cerevisiae<\/em>\u306f\u30b0\u30eb\u30b3\u30fc\u30b9\u4f9b\u7d66\u304c\u306a\u304f\u306a\u3063\u305f\u3068\u304dEtOH\u3092\u7528\u3044\u305f\u597d\u6c17\u5897\u6b96\u306b\u30b7\u30d5\u30c8\u3059\u308b\uff08diauxic shift\u3068\u547c\u3070\u308c\u308b\uff09\u3002<\/li>\n<\/ul>\n<div><span style=\"color: #444444; font-size: 20px; line-height: 30px;\">\u30b2\u30ce\u30df\u30af\u30b9<\/span><\/div>\n<ul>\n<li>\u4e21\u9175\u6bcd\n<ul>\n<li>DNA ~60% A+T<\/li>\n<li>\u901a\u5e38\u306e\u30b3\u30a2\u30d2\u30b9\u30c8\u30f3; histone H1\u306b\u975e\u5e38\u306b\u8fd1\u3044\u86cb\u767d\u8cea\u306f\u5b58\u5728\u3057\u306a\u3044\u3002<\/li>\n<li>\u6027\u6c7a\u5b9a\u9818\u57df\u3001\u30c6\u30ed\u30e1\u30a2\u3001rDNA\u9818\u57df\u3067\u8ee2\u5199\u306e\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u304c\u898b\u3089\u308c\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li><em>S. cerevisiae<\/em>\n<ul>\n<li>\u67d3\u8272\u4f53\u6570\u300016\u672c<\/li>\n<li>\u5168\u30b2\u30ce\u30e0\u5869\u57fa\u914d\u5217\u89e3\u8aad\u6e08\u307f (12 Mb; \u7d046,000\u907a\u4f1d\u5b50)<\/li>\n<li>\u5c0f\u3055\u306a\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2 (~120 bp)\u3001\u30e6\u30cb\u30fc\u30af\uff1b \u86cb\u767d\u8cea\u3068\u7d50\u5408\u3059\u308b3\u3064\u306e\u9818\u57df\u304c\u3042\u308a\u3001\u305d\u308c\u304c\u5206\u914d\u306e\u305f\u3081\u306b\u5358\u4e00\u306e\u5fae\u5c11\u7ba1\u3068\u7d50\u5408\u3059\u308b\u3002\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u9818\u57df\u3067\u306e\u8ee2\u5199\u306e\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u306f\u306a\u3044\u3002<\/li>\n<li>\u30c6\u30ed\u30e1\u30a2\u306e\u7e70\u308a\u8fd4\u3057\u914d\u5217\u306f (TG1-3)n [\u30d2\u30c8\u306e\u30c6\u30ed\u30e1\u30a2\u914d\u5217\u306f (TTAGGG)n]<\/li>\n<li>\u8907\u88fd\u8d77\u70b9 (ARS\u56e0\u5b50) \u306f\u3001100-150 bp\u3002<\/li>\n<li>\u30a4\u30f3\u30c8\u30ed\u30f3\u306e\u6570\u306f\u3001300\u7a0b\u5ea6\u3002\u7d044.5\uff05\u306e\u907a\u4f1d\u5b50\u304c\u30a4\u30f3\u30c8\u30ed\u30f3\u3092\u6301\u3063\u3066\u3044\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li><em>S. pombe<\/em>\n<ul>\n<li>3\u672c\u306e\u5de8\u5927\u306a\u67d3\u8272\u4f53<\/li>\n<li>\u5168\u30b2\u30ce\u30e0\u5869\u57fa\u914d\u5217\u89e3\u8aad\u6e08\u307f (14 Mb; \u7d045,000\u907a\u4f1d\u5b50)<\/li>\n<li>\u5927\u304d\u306a\u30bb\u30f3\u30c8\u30ed\u30e1\u30a2 (40-100 kb)\u3001\u307b\u3068\u3093\u3069\u306f\u7e70\u308a\u8fd4\u3057\u914d\u5217\uff1b \u4e00\u672c\u4ee5\u4e0a\u306e\u5fae\u5c11\u7ba1\u3068\u7d50\u5408\uff1b \u30bb\u30f3\u30c8\u30ed\u30e1\u30a2\u9818\u57df\u306b\u3042\u308b\u907a\u4f1d\u5b50\u306f\u901a\u5e38\u30b5\u30a4\u30ec\u30f3\u30b7\u30f3\u30b0\u3092\u53d7\u3051\u3066\u3044\u308b\u3002<\/li>\n<li>\u30c6\u30ed\u30e1\u30a2\u306f\u4e00\u898b\u8907\u96d1 (T1-3AC0-1A0-2C0-1G1-8)n\u3000\u3060\u304c\u3001\u4e00\u822c\u7684\u306a\u7e70\u308a\u8fd4\u3057\u914d\u5217\u306f TTACAGG [\u30d2\u30c8\u306e\u30c6\u30ed\u30e1\u30a2\u914d\u5217\u306f (TTAGGG)n]<\/li>\n<li>\u8907\u88fd\u8d77\u70b9 (ARS\u56e0\u5b50) \u306f\u3001500-1500 bp\u3002<\/li>\n<li>\u30a4\u30f3\u30c8\u30ed\u30f3\u306e\u6570\u306f\u30015000\u7a0b\u5ea6\u3002\u7d0440\uff05\u306e\u907a\u4f1d\u5b50\u304c\u30a4\u30f3\u30c8\u30ed\u30f3\u3092\u6301\u3063\u3066\u3044\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3 id=\"content_1_7\">\u67d3\u8272\u4f53\u5916\u56e0\u5b50<\/h3>\n<h4 id=\"content_1_8\">\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2<\/h4>\n<ul>\n<li>\u51fa\u82bd\u9175\u6bcd\u304c\u597d\u6c17\u3068\u5acc\u6c17\u6761\u4ef6\u3067\u5897\u6b96\u3067\u304d\u308b\u3068\u3044\u3046\u3053\u3068\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u547c\u5438\u6a5f\u80fd\u306f\u5fc5\u9808\u3067\u306a\u3044\u3053\u3068\u3092\u610f\u5473\u3057\u3066\u3044\u308b\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u306f\u671b\u307f\u306e\u901a\u308a\u306b\u5909\u7570\u3092\u5c0e\u5165\u3067\u304d\u308b\u305f\u3081\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u907a\u4f1d\u5b66\u304c\u5bb9\u6613\u306b\u7814\u7a76\u53ef\u80fd\u3067\u3042\u308b\u3002<\/li>\n<li>\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u30b2\u30ce\u30e0\u3092\u5168\u3066\u6b20\u5931\u3055\u3048\u308b\u3053\u3068\u306f\u53ef\u80fd\u3067\u3042\u308b\u304c\u3001\u6838\u306b\u30b3\u30fc\u30c9\u3055\u308c\u305f\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u819c\u306f\u5168\u3066\u306e\u6761\u4ef6\u3067\u751f\u5b58\u306b\u5fc5\u9808\u3067\u3042\u308a\u6b20\u5931\u3055\u305b\u308b\u3053\u3068\u306f\u51fa\u6765\u306a\u3044\u3002<\/li>\n<li><em>S.cerevisiae<\/em>\u306e\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u5909\u7570\u4f53\u306f\u3001\u5897\u6b96\u9045\u5ef6\u3092\u793a\u3059\u3082\u306e\u306e\uff08\u767a\u9175\u304c\u53ef\u80fd\u306a\uff09\u30b0\u30eb\u30b3\u30fc\u30b9\u3067\u306f\u5897\u6b96\u3067\u304d\u308b\u304c\u3001\uff08\u9178\u7d20\u547c\u5438\u3067\u3057\u304b\u4ee3\u8b1d\u3067\u304d\u306a\u3044\uff09\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\u3067\u306f\u5897\u6b96\u3067\u304d\u306a\u3044\u3002<\/li>\n<li>\u5206\u88c2\u9175\u6bcd\u306e\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u306f\u91ce\u751f\u578b\u7d30\u80de\u3067\u306f\u5fc5\u9808\u3067\u3042\u308b\u304c\u3001\u3042\u308b\u7a2e\u306e\u6838\u306b\u30b3\u30fc\u30c9\u3055\u308c\u305f\u907a\u4f1d\u5b50\u306e\u5909\u7570\u306b\u3088\u3063\u3066\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u306e\u6b20\u5931\u3057\u305f\u5909\u7570\u682a\u3092\u53d6\u5f97\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308b\u3002<\/li>\n<\/ul>\n<h4 id=\"content_1_9\">\u30d7\u30e9\u30b9\u30df\u30c9<\/h4>\n<ul>\n<li><em>S. cerevisiae<\/em>\n<ul>\n<li>2 \u00b5m circle\n<ul>\n<li>6.3 kbp<\/li>\n<li>60-100 \u30b3\u30d4\u30fc\uff0f\u7d30\u80de<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li><em>S. pombe<\/em>\n<ul>\n<li>\u5929\u7136\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u77e5\u3089\u308c\u3066\u3044\u306a\u3044\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h4 id=\"content_1_10\"><em>S. cerevisiae<\/em>\u306e\u30e1\u30f3\u30c7\u30eb\u5247\u306b\u5f93\u308f\u306a\u3044\u56e0\u5b50<\/h4>\n<ul>\n<li>\u30d7\u30ea\u30aa\u30f3\u69d8\u86cb\u767d\u8cea\n<ul>\n<li>\u512a\u6027<\/li>\n<li>\u6838\u5916<\/li>\n<li>\u30ad\u30e9\u30fc\u7c92\u5b50\u3084\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3068\u306f\u7121\u95a2\u4fc2\u3002<\/li>\n<li>\u81ea\u7136\u64ad\u7a2e<\/li>\n<li>\u4f8b:\n<ul>\n<li>[PSI+]\/[psi-]<\/li>\n<li>\u30d5\u30ec\u30fc\u30e0\u30b7\u30d5\u30c8\u306e\u983b\u5ea6\u304c\u4e0a\u304c\u308a\u30b5\u30d7\u30ec\u30c3\u30b7\u30e7\u30f3\u304c\u304a\u304d\u308b\u3002<\/li>\n<li>\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u4e56\u96e2\u56e0\u5b50Sup35p\u306e\u69cb\u9020\u5909\u7570\u4f53\uff08\u51dd\u96c6\u4f53\uff09<\/li>\n<li>[URE3]\/[ure3-]<\/li>\n<li>\u7a92\u7d20\u4ee3\u8b1d\u306b\u5f71\u97ff<\/li>\n<li>\u7a92\u7d20\u4ee3\u8b1d\u5236\u5fa1\u56e0\u5b50Ure2p\u306e\u69cb\u9020\u5909\u7570\u4f53\uff08\u51dd\u96c6\u4f53\uff09<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<div><span style=\"color: #444444; font-size: 17px; line-height: 25px;\">\u30ad\u30e9\u30fc\u7c92\u5b50<\/span><\/div>\n<ul>\n<li>\u4e0d\u5b8c\u5168\u306adsRNA\u30a6\u30a4\u30eb\u30b9\u306b\u30b3\u30fc\u30c9\u3055\u308c\u305f\u81f4\u6b7b\u6027\u6bd2\u7d20<\/li>\n<li>Jeremy Bruenn\u306b\u3088\u3063\u3066\u8abf\u3079\u3089\u308c\u305f\u3002<\/li>\n<\/ul>\n<h3 id=\"content_1_12\">\u907a\u4f1d\u5b50\u8868\u8a18\u6cd5<\/h3>\n<ul>\n<li><em>S. cerevisiae<\/em>\n<ul>\n<li>\u907a\u4f1d\u5b50\u540d\u306f3\u3064\u306e\u6587\u5b57\u3068\u6570\u5b57\u3067\u8868\u3059\u3002<\/li>\n<li>\u512a\u6027\u306e\u30a2\u30ea\u30eb\u306f\u5927\u6587\u5b57\u3067\u3001\u52a3\u6027\u306e\u30a2\u30ea\u30eb\u306f\u5c0f\u6587\u5b57\u3067\u8868\u3059\u3002\u901a\u5e38\u91ce\u751f\u578b\u306f\u512a\u6027\u3067\u3042\u308b\uff08\u4f8b\u5916\u3082\u5b58\u5728\u3059\u308b\uff09\u3002<\/li>\n<li>\u907a\u4f1d\u5b50\u540d\u306f\u659c\u4f53\u3067\u8868\u3059\uff08<em>YFG1<\/em>\uff09\u3002<\/li>\n<li>\u91ce\u751f\u578b\u306f\u4efb\u610f\u3067\uff0b\u8a18\u53f7\u3092\u3064\u3051\u308b\u3053\u3068\u304c\u51fa\u6765\u308b\uff08<em>YFG1<\/em>+\uff09\u3002<\/li>\n<li>\u5909\u7570\u30a2\u30ea\u30eb\u306f\u30a2\u30ea\u30eb\u7279\u7570\u7684\u306a\u6570\u5b57\u3067\u793a\u3055\u308c\u308b\uff08<em>yfg1-1<\/em>\uff09\u3002<\/li>\n<li>\u907a\u4f1d\u5b50\u7834\u58ca\u306f\u3001<em>yfg1<\/em>::<em>URA3<\/em> \u3042\u308b\u3044\u306f\u00a0<em>yfg1<\/em>\u0394 \u3068\u8868\u3055\u308c\u308b\u3002<\/li>\n<li>\u86cb\u767d\u8cea\u306f\u4e00\u90e8\u3092\u5c0f\u6587\u5b57\u3067\u3001\u659c\u4f53\u306b\u305b\u305a\u8868\u3059\uff08Yfg1 or Yfg1p\uff09\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li><em>S. pombe<\/em>\n<ul>\n<li>\u907a\u4f1d\u5b50\u540d\u306f3\u3064\u306e\u5c0f\u6587\u5b57\u3068\u6570\u5b57\u3067\u8868\u3059\u3002<\/li>\n<li>\u91ce\u751f\u578b\u306f\uff0b\u8a18\u53f7\u3067\u8868\u3059\uff08<em>yfg1<\/em>+\uff09\u3002<\/li>\n<li>\u5909\u7570\u30a2\u30ea\u30eb\u306f\u30a2\u30ea\u30eb\u7279\u7570\u7684\u306a\u6570\u5b57\u3067\u793a\u3055\u308c\u308b\uff08<em>yfg1-1<\/em>\uff09\u3002<\/li>\n<li>\u907a\u4f1d\u5b50\u7834\u58ca\u306f\u3001<em>yfg1<\/em>::<em>ura4<\/em> \u3042\u308b\u3044\u306f\u00a0<em>yfg1<\/em>\u0394 \u3068\u8868\u3055\u308c\u308b\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u51fa\u82bd\u9175\u6bcd\u00a0Saccharomy&#8230;<\/p>\n","protected":false},"author":3,"featured_media":3479,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[9],"tags":[],"class_list":["post-97","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-9"],"_links":{"self":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/97","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=97"}],"version-history":[{"count":6,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/97\/revisions"}],"predecessor-version":[{"id":3480,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/posts\/97\/revisions\/3480"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=\/wp\/v2\/media\/3479"}],"wp:attachment":[{"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=97"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=97"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/tenure5.vbl.okayama-u.ac.jp\/HM_blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=97"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}