LOCUS pRS314_addgene_ 4783 bp ds-DNA circular 28-APR-2009 DEFINITION pRS314 Sequence sequence KEYWORDS . SOURCE pRS314 Sequence sequence ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene Vector Database pRS314 Sequence sequence at www.addgene.org COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 1..4783 /organism="Addgene Vector Database pRS314 Sequence sequence" /mol_type="other DNA" /label=source:Addgene Vector Database pRS314 Sequence seq /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature complement(29..51) /label=pGEX_3_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 CDS 468..1142 /gene="TRP1" /note="Orf frame 3" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" /label=TRP1 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 gene 468..1142 /gene="TRP1" /label=TRP1(1) /ApEinfo_label=TRP1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink rep_origin complement(1371..1677) /label=f1_origin /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature complement(1710..1858) /label=lacZ_a /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature 1824..1846 /label=M13_pUC_fwd_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature 1839..1855 /label=M13_forward20_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 promoter 1862..1880 /label=T7_promoter /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature 1913..1929 /label=pBluescriptSK_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature complement(1964..1980) /label=pBluescriptKS_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 promoter complement(2008..2027) /label=T3_promoter /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature complement(2044..2062) /label=M13_reverse_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature complement(2061..2083) /label=M13_pUC_rev_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 rep_origin complement(2435..3054) /label=pBR322_origin /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 CDS complement(3209..4069) /gene="Ampicillin" /note="Orf frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" /label=Ampicillin /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 gene complement(3209..4069) /gene="Ampicillin" /label=Ampicillin(1) /ApEinfo_label=Ampicillin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter complement(4111..4139) /label=AmpR_promoter /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature 4201..4719 /label=CEN6_ARS4 /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgcctg cggtgtgaaa 1201 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt 1261 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 1321 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 1381 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 1441 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 1501 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 1561 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 1621 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 1681 gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 1741 ggtgcgggcc tcttcgctat tacgccagct ggcgaagggg ggatgtgctg caaggcgatt 1801 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 1861 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac 1921 tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga 1981 gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt 2041 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 2101 taggagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat 2161 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 2221 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 2281 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 2341 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 2401 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 2461 tcggcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 2521 caggactata aagataccag gcgttccccc ctggaagctc cctcgtgcgc tctcctgttc 2581 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2641 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2701 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2761 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2821 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2881 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2941 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3001 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3061 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 3241 cagcgatctg tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta 3301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 3361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 3421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 3481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 3541 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3601 catgatcccc catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 3661 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 3721 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 3781 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 3841 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 3901 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 3961 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 4021 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4081 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 4141 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 4201 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata 4261 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa 4321 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc 4381 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat 4441 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc 4501 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt 4561 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa 4621 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag 4681 atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg 4741 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc //