LOCUS pRS423_addgene_ 5797 bp ds-DNA circular 28-APR-2009 DEFINITION pRS423 Sequence sequence KEYWORDS . SOURCE pRS423 Sequence sequence ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene Vector Database pRS423 Sequence sequence at www.addgene.org COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 1..5797 /organism="Addgene Vector Database pRS423 Sequence sequence" /mol_type="other DNA" /label=source:Addgene Vector Database pRS423 Sequence seq /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature complement(29..51) /label=pGEX_3_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 gene 504..1160 /gene="HIS3" /label=HIS3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink CDS 504..1163 /gene="HIS3" /note="Orf frame 3" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVA EQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQ AFKEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIP HFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVL M*" /label=HIS3(1) /ApEinfo_label=HIS3 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 rep_origin complement(1555..1861) /label=f1_origin /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature complement(1894..2037) /label=lacZ_a /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature 2008..2030 /label=M13_pUC_fwd_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature 2023..2039 /label=M13_forward20_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 promoter 2049..2067 /label=T7_promoter /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature 2092..2108 /label=pBluescriptKS_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature complement(2143..2159) /label=pBluescriptSK_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 promoter complement(2195..2214) /label=T3_promoter /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature complement(2231..2249) /label=M13_reverse_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 misc_feature complement(2248..2270) /label=M13_pUC_rev_primer /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 rep_origin complement(2622..3241) /label=pBR322_origin /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 CDS complement(3396..4256) /gene="Ampicillin" /note="Orf frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" /label=Ampicillin /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 gene complement(3396..4256) /gene="Ampicillin" /label=Ampicillin(1) /ApEinfo_label=Ampicillin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink promoter complement(4298..4326) /label=AmpR_promoter /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 rep_origin complement(4388..5359) /label=2micron2_origin /ApEinfo_fwdcolor=#996633 /ApEinfo_revcolor=#996633 misc_feature complement(5351..5398) /label=FRT /ApEinfo_fwdcolor=#ff0081 /ApEinfo_revcolor=#ff0081 ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg 2101 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag 2161 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc 2221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 2281 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa 2341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 2401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 2461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 2521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 2581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 2641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 2701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 2761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 2821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 2881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 2941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 3001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 3061 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 3121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 3181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 3241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 3301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 3361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 3421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 3481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 3541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 3601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 3661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 3721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 3781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 3841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 3901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 3961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 4021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 4081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 4141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 4201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 4261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 4321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 4381 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct 4441 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc 4501 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga 4561 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 4621 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc 4681 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc 4741 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct 4801 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg 4861 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta 4921 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat 4981 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat 5041 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt 5101 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag 5161 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc 5221 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt 5281 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca 5341 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa 5401 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac 5461 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg 5521 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag 5581 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc 5641 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt 5701 caatgctatc atttcctttg atattggatc atctaagaaa ccattattat catgacatta 5761 acctataaaa ataggcgtat cacgaggccc tttcgtc //